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SLC13A5 (NM_177550) Human Tagged ORF Clone

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SKU
RC211155
SLC13A5 (Myc-DDK-tagged)-Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $529.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC13A5
Synonyms DEE25; EIEE25; INDY; mIndy; NACT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211155 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGGCGCTGAGCTATGTCTCCAAGTTCAAGTCCTTCGTGATCTTGTTCGTCACCCCGCTCCTGC
TGCTGCCACTCGTCATTCTGATGCCCGCCAAGTTTGTCAGGTGTGCCTACGTCATCATCCTCATGGCCAT
TTACTGGTGCACAGAAGTCATCCCTCTGGCTGTCACCTCTCTCATGCCTGTCTTGCTTTTCCCACTCTTC
CAGATTCTGGACTCCAGGCAGGTGTGTGTCCAGTACATGAAGGACACCAACATGCTGTTCCTGGGCGGCC
TCATCGTGGCCGTGGCTGTGGAGCGCTGGAACCTGCACAAGAGGATCGCCCTGCGCACGCTCCTCTGGGT
GGGGGCCAAGCCTGCACGGCTGATGCTGGGCTTCATGGGCGTCACAGCCCTCCTGTCCATGTGGATCAGT
AACACGGCAACCACGGCCATGATGGTGCCCATCGTGGAGGCCATATTGCAGCAGATGGAAGCCACAAGCG
CAGCCACCGAGGCCGGCCTGGAGCTGGTGGACAAGGGCAAGGCCAAGGAGCTGCCAGGGAGTCAAGTGAT
TTTTGAAGGCCCCACTCTGGGGCAGCAGGAAGACCAAGAGCGGAAGAGGTTGTGTAAGGCCATGACCCTG
TGCATCTGCTACGCGGCCAGCATCGGGGGCACCGCCACCCTGACCGGGACGGGACCCAACGTGGTGCTCC
TGGGCCAGATGAACGAGTTGTTTCCTGACAGCAAGGACCTCGTGAACTTTGCTTCCTGGTTTGCATTTGC
CTTTCCCAACATGCTGGTGATGCTGCTGTTCGCCTGGCTGTGGCTCCAGTTTGTTTACATGAGATTCAAT
TTTAAAAAGTCCTGGGGCTGCGGGCTAGAGAGCAAGAAAAACGAGAAGGCTGCCCTCAAGGTGCTGCAGG
AGGAGTACCGGAAGCTGGGGCCCTTGTCCTTCGCGGAGATCAACGTGCTGATCTGCTTCTTCCTGCTGGT
CATCCTGTGGTTCTCCCGAGACCCCGGCTTCATGCCCGGCTGGCTGACTGTTGCCTGGGTGGAGGGTGAG
ACAAAGTATGTCTCCGATGCCACTGTGGCCATCTTTGTGGCCACCCTGCTATTCATTGTGCCTTCACAGA
AGCCCAAGTTTAACTTCCGCAGCCAGACTGAGGAAGAAAGGAAAACTCCATTTTATCCCCCTCCCCTGCT
GGATTGGAAGGTAACCCAGGAGAAAGTGCCCTGGGGCATCGTGCTGCTACTAGGGGGCGGATTTGCTCTG
GCTAAAGGATCCGAGGCCTCGGGGCTGTCCGTGTGGATGGGGAAGCAGATGGAGCCCTTGCACGCAGTGC
CCCCGGCAGCCATCACCTTGATCTTGTCCTTGCTCGTTGCCGTGTTCACTGAGTGCACAAGCAACGTGGC
CACCACCACCTTGTTCCTGCCCATCTTTGCCTCCATGTCTCGCTCCATCGGCCTCAATCCGCTGTACATC
ATGCTGCCCTGTACCCTGAGTGCCTCCTTTGCCTTCATGTTGCCTGTGGCCACCCCTCCAAATGCCATCG
TGTTCACCTATGGGCACCTCAAGGTTGCTGACATGGTGAAAACAGGAGTCATAATGAACATAATTGGAGT
CTTCTGTGTGTTTTTGGCTGTCAACACCTGGGGACGGGCCATATTTGACTTGGATCATTTCCCTGACTGG
GCTAATGTGACACATATTGAGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211155 protein sequence
Red=Cloning site Green=Tags(s)

MASALSYVSKFKSFVILFVTPLLLLPLVILMPAKFVRCAYVIILMAIYWCTEVIPLAVTSLMPVLLFPLF
QILDSRQVCVQYMKDTNMLFLGGLIVAVAVERWNLHKRIALRTLLWVGAKPARLMLGFMGVTALLSMWIS
NTATTAMMVPIVEAILQQMEATSAATEAGLELVDKGKAKELPGSQVIFEGPTLGQQEDQERKRLCKAMTL
CICYAASIGGTATLTGTGPNVVLLGQMNELFPDSKDLVNFASWFAFAFPNMLVMLLFAWLWLQFVYMRFN
FKKSWGCGLESKKNEKAALKVLQEEYRKLGPLSFAEINVLICFFLLVILWFSRDPGFMPGWLTVAWVEGE
TKYVSDATVAIFVATLLFIVPSQKPKFNFRSQTEEERKTPFYPPPLLDWKVTQEKVPWGIVLLLGGGFAL
AKGSEASGLSVWMGKQMEPLHAVPPAAITLILSLLVAVFTECTSNVATTTLFLPIFASMSRSIGLNPLYI
MLPCTLSASFAFMLPVATPPNAIVFTYGHLKVADMVKTGVIMNIIGVFCVFLAVNTWGRAIFDLDHFPDW
ANVTHIET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177550
ORF Size 1704 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177550.5
RefSeq Size 3316 bp
RefSeq ORF 1707 bp
Locus ID 284111
UniProt ID Q86YT5
Cytogenetics 17p13.1
Protein Families Transmembrane
MW 63.1 kDa
Summary This gene encodes a protein belonging to the solute carrier family 13 group of proteins. This family member is a sodium-dependent citrate cotransporter that may regulate metabolic processes. Mutations in this gene cause early infantile epileptic encephalopathy 25. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2014
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Documents
Resources
"NM_177550" in other vectors (6)
SKU Description Size Price
RC211155L1 Lenti ORF clone of Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1, Myc-DDK-tagged 10 ug
$829.00
RC211155L2 Lenti ORF clone of Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1, mGFP tagged 10 ug
$829.00
RC211155L3 Lenti ORF clone of Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1, Myc-DDK-tagged 10 ug
$829.00
RC211155L4 Lenti ORF clone of Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1, mGFP tagged 10 ug
$829.00
RG211155 SLC13A5 (tGFP-tagged) - Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1 10 ug
$489.00 $729.00
SC309250 SLC13A5 (untagged)-Human solute carrier family 13 (sodium-dependent citrate transporter), member 5 (SLC13A5), transcript variant 1 10 ug
$530.00

Plasmid Map for RC211155

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211155
Circular map for RC211155

file-alt Citations

SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype
Rigby, Orefice, Lawton et al
Brain Commun (2022) 4 (1), fcac002 DOI: 10.1093/braincomms/fcac002
Cited by 22
AI Summary: In this study, OriGene's SLC13A5 Myc-DDK-tagged cDNA (Cat# RC208383) and SLC13A5 shRNA plasmid (Cat# TL516969) were used to manipulate SLC13A5 expression in neuronal cells, enabling the investigation of how altered SLC13A5 levels contribute to disrupted white matter integrity and autistic-like behaviors in mice.
Figure
SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype
Rigby, Orefice, Lawton et al
Brain Commun (2022) 4 (1), fcac002 DOI: 10.1093/braincomms/fcac002
Cited by 22
AI Summary: OriGene's SLC13A5-pCMV6 plasmid (RC211155) provided the human SLC13A5 cDNA used to generate constructs for neuron-specific overexpression studies in this paper.
Snippet: Briefly, human cDNA was isolated by PCR from SLC13A5-pCMV6 plasmid (Origene;RC211155) and subcloned into pTRE-Tight plasmid (Takara Bio, Inc.)
Showing 1-2 of 2 papers.
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