GAS8 (NM_001481) Human Tagged ORF Clone

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SKU
RC211150
GAS8 (Myc-DDK-tagged)-Human growth arrest-specific 8 (GAS8), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GAS8
Synonyms CILD33; DRC4; GAS11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211150 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACCGAAAAAGAAAGGGAAGAAAGGCAAAGCCAAAGGCACCCCGATTGTCGATGGGCTCGCTCCAG
AGGACATGAGCAAGGAGCAGGTGGAGGAGCATGTCAGCCGCATCCGGGAGGAGCTGGACCGCGAGCGGGA
GGAACGAAACTACTTCCAGCTGGAGCGGGACAAGATCCACACCTTCTGGGAGATCACACGGAGGCAGCTG
GAGGAGAAGAAGGCTGAGCTGCGGAACAAAGACCGGGAGATGGAAGAAGCCGAGGAGAGGCACCAGGTGG
AGATCAAGGTGTACAAGCAGAAAGTGAAGCACCTGCTATATGAGCACCAGAACAACCTGACAGAGATGAA
GGCTGAGGGCACTGTAGTCATGAAGCTGGCACAGAAAGAGCACCGCATACAGGAGAGTGTGCTGCGCAAG
GACATGCGGGCACTGAAGGTGGAGCTCAAGGAGCAGGAGCTGGCCAGTGAGGTGGTGGTGAAGAACCTGC
GGCTGAAACACACCGAGGAGATCACCAGGATGCGGAATGATTTTGAGAGGCAAGTTCGAGAAATTGAGGC
CAAGTATGATAAGAAGATGAAGATGCTGAGGGACGAACTCGACTTGCGGAGAAAGACTGAGCTCCACGAA
GTGGAGGAGAGGAAGAATGGCCAGATCCACACGCTGATGCAGCGCCACGAGGAGGCCTTCACCGACATTA
AGAACTACTACAACGACATCACCCTCAACAACCTGGCCCTCATCAACTCCCTCAAGGAGCAGATGGAGGA
CATGCGGAAGAAGGAGGACCACCTGGAGAGGGAGATGGCAGAGGTGTCTGGGCAGAACAAGCGCCTGGCA
GACCCTCTCCAGAAGGCTCGGGAGGAGATGAGCGAGATGCAGAAACAGCTCGCAAACTACGAGAGGGACA
AGCAGATCCTGCTTTGCACAAAAGCCCGTTTGAAAGTCAGGGAGAAAGAGCTGAAAGACCTGCAGTGGGA
GCATGAAGTGTTAGAGCAGCGATTCACCAAGGTGCAGCAGGAGCGGGACGAGCTCTATCGGAAGTTCACC
GCAGCCATCCAGGAGGTGCAGCAGAAGACAGGGTTCAAGAACCTCGTGCTAGAACGCAAGCTGCAGGCTC
TGAGCGCCGCTGTGGAGAAGAAGGAGGTGCAGTTCAACGAGGTCCTGGCTGCCTCTAACCTGGACCCTGC
AGCCCTGACGCTGGTGTCCCGCAAGCTGGAGGATGTTCTTGAGTCGAAGAACAGCACCATCAAGGACCTG
CAGTATGAGCTGGCCCAGGTCTGTAAGGCCCATAACGACCTGCTGCGCACGTATGAGGCAAAGCTGCTGG
CCTTCGGGATCCCTCTGGACAACGTGGGCTTCAAGCCCTTGGAAACAGCTGTGATCGGACAGACACTGGG
CCAGGGCCCCGCGGGACTGGTGGGCACCCCGACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211150 protein sequence
Red=Cloning site Green=Tags(s)

MAPKKKGKKGKAKGTPIVDGLAPEDMSKEQVEEHVSRIREELDREREERNYFQLERDKIHTFWEITRRQL
EEKKAELRNKDREMEEAEERHQVEIKVYKQKVKHLLYEHQNNLTEMKAEGTVVMKLAQKEHRIQESVLRK
DMRALKVELKEQELASEVVVKNLRLKHTEEITRMRNDFERQVREIEAKYDKKMKMLRDELDLRRKTELHE
VEERKNGQIHTLMQRHEEAFTDIKNYYNDITLNNLALINSLKEQMEDMRKKEDHLEREMAEVSGQNKRLA
DPLQKAREEMSEMQKQLANYERDKQILLCTKARLKVREKELKDLQWEHEVLEQRFTKVQQERDELYRKFT
AAIQEVQQKTGFKNLVLERKLQALSAAVEKKEVQFNEVLAASNLDPAALTLVSRKLEDVLESKNSTIKDL
QYELAQVCKAHNDLLRTYEAKLLAFGIPLDNVGFKPLETAVIGQTLGQGPAGLVGTPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001481
ORF Size 1434 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001481.3
RefSeq Size 3185 bp
RefSeq ORF 1437 bp
Locus ID 2622
UniProt ID O95995, A0A384MR00
Cytogenetics 16q24.3
MW 56.4 kDa
Summary This gene includes 11 exons spanning 25 kb and maps to a region of chromosome 16 that is sometimes deleted in breast and prostrate cancer. The second intron contains an apparently intronless gene, C16orf3, that is transcribed in the opposite orientation. This gene is a putative tumor suppressor gene. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2013
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Documents
Resources
"NM_001481" in other vectors (4)
SKU Description Size Price
RC211150L3 Lenti ORF clone of Human growth arrest-specific 8 (GAS8), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211150L4 Lenti ORF clone of Human growth arrest-specific 8 (GAS8), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211150 GAS8 (tGFP-tagged) - Human growth arrest-specific 8 (GAS8), transcript variant 1 10 ug
$489.00 $657.00
SC303030 GAS8 (untagged)-Human growth arrest-specific 8 (GAS8), transcript variant 1 10 ug
$503.00

Plasmid Map for RC211150

Download sequence for

Cloning scheme for RC211150

Circular map for RC211150

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