OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

HSPA12B (NM_052970) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC211102
HSPA12B (Myc-DDK-tagged)-Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $628.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "HSPA12B or NM_052970"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSPA12B
Synonyms C20orf60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGCTGTCCCGGAGATGGGCCTGCAGGGGCTGTACATCGGCTCCAGCCCGGAGCGGTCCCCAGTGC
CTAGCCCACCCGGCTCCCCGAGGACCCAGGAAAGCTGCGGCATTGCCCCCCTCACACCCTCGCAGTCTCC
AAAACCCGAGGTCCGAGCCCCCCAGCAGGCCTCCTTCTCTGTGGTGGTGGCCATTGACTTCGGCACCACG
TCTAGTGGCTATGCTTTCAGCTTTGCCAGTGACCCTGAGGCCATCCACATGATGAGGAAATGGGAGGGCG
GAGACCCGGGCGTGGCCCACCAGAAGACCCCGACCTGCCTGCTGCTGACTCCGGAGGGCGCCTTCCACAG
CTTTGGCTACACCGCCCGCGATTACTACCATGACCTGGACCCCGAAGAGGCGCGGGACTGGCTCTACTTC
GAGAAGTTCAAGATGAAGATCCACAGCGCCACGGATCTCACCTTGAAGACCCAGCTAGAGGCAGTAAATG
GAAAGACGATGCCCGCCCTGGAGGTGTTCGCCCATGCCCTGCGCTTCTTCAGGGAGCACGCCCTTCAGGA
GCTGAGGGAGCAGAGCCCATCGCTGCCAGAGAAGGACACTGTGCGCTGGGTGTTGACGGTGCCTGCCATC
TGGAAACAGCCAGCCAAGCAGTTCATGCGGGAGGCTGCCTACCTGGCTGGACTAGTGTCCCGAGAGAATG
CAGAGCAGCTACTCATCGCCCTGGAGCCCGAGGCCGCCTCGGTATACTGCCGCAAGCTGCGCCTGCACCA
GCTCCTGGACCTGAGTGGCCGGGCCCCAGGTGGTGGGCGCCTGGGTGAGCGCCGCTCCATCGACTCCAGC
TTCCGTCAGGCTCGGGAGCAGCTGCGAAGGTCCCGCCACAGCCGCACGTTCCTGGTGGAGTCAGGCGTAG
GAGAGCTGTGGGCAGAGATGCAAGCAGGAGACCGCTACGTGGTGGCCGACTGCGGCGGAGGCACCGTGGA
CCTGACGGTGCACCAGCTGGAGCAGCCCCATGGCACCCTCAAGGAGCTCTACAAGGCATCTGGGGGCCCT
TATGGCGCGGTGGGCGTGGACCTGGCCTTCGAGCAGCTGCTGTGCCGCATCTTCGGCGAGGACTTCATCG
CCACCTTCAAAAGGCAACGGCCGGCAGCCTGGGTAGATCTGACCATCGCCTTCGAGGCTCGCAAGCGCAC
TGCTGGCCCACACCGTGCAGGGGCGCTCAACATCTCGCTGCCCTTCTCCTTCATTGACTTCTACCGCAAG
CAGCGGGGCCACAACGTGGAGACCGCTCTGCGCAGGAGCAGCGTGAACTTCGTGAAGTGGTCCTCACAGG
GGATGCTCCGAATGTCTTGTGAAGCCATGAACGAGCTCTTTCAGCCCACCGTCAGCGGGATCATCCAGCA
CATAGAGGCCCTGCTGGCACGGCCGGAGGTGCAGGGTGTGAAGCTGCTGTTCCTAGTGGGCGGCTTCGCC
GAGTCAGCGGTGCTGCAGCACGCGGTGCAGGCGGCGCTGGGCGCCCGCGGTCTGCGTGTCGTGGTCCCGC
ACGACGTGGGCCTCACCATCCTCAAAGGCGCGGTGCTGTTCGGCCAGGCGCCGGGCGTGGTGCGGGTCCG
CCGCTCGCCGCTCACCTATGGCGTGGGCGTGCTCAACCGCTTTGTGCCTGGGCGCCACCCGCCCGAAAAG
CTGCTGGTTCGCGACGGCCGCCGCTGGTGCACCGACGTCTTCGAGCGCTTCGTGGCCGCCGAGCAGTCGG
TGGCCCTGGGCGAGGAGGTGCGGCGCAGCTACTGCCCGGCGCGTCCCGGCCAGCGGCGCGTACTCATCAA
CCTGTACTGCTGCGCGGCAGAGGATGCGCGCTTCATCACCGACCCCGGCGTGCGCAAATGTGGCGCGCTC
AGCCTCGAGCTTGAGCCCGCCGACTGCGGCCAGGACACCGCCGGCGCGCCTCCCGGCCGCCGCGAGATCC
GCGCCGCCATGCAGTTTGGCGACACCGAAATTAAGGTCACCGCCGTCGACGTCAGCACCAATCGCTCCGT
GCGCGCGTCCATCGACTTTCTTTCCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211102 protein sequence
Red=Cloning site Green=Tags(s)

MLAVPEMGLQGLYIGSSPERSPVPSPPGSPRTQESCGIAPLTPSQSPKPEVRAPQQASFSVVVAIDFGTT
SSGYAFSFASDPEAIHMMRKWEGGDPGVAHQKTPTCLLLTPEGAFHSFGYTARDYYHDLDPEEARDWLYF
EKFKMKIHSATDLTLKTQLEAVNGKTMPALEVFAHALRFFREHALQELREQSPSLPEKDTVRWVLTVPAI
WKQPAKQFMREAAYLAGLVSRENAEQLLIALEPEAASVYCRKLRLHQLLDLSGRAPGGGRLGERRSIDSS
FRQAREQLRRSRHSRTFLVESGVGELWAEMQAGDRYVVADCGGGTVDLTVHQLEQPHGTLKELYKASGGP
YGAVGVDLAFEQLLCRIFGEDFIATFKRQRPAAWVDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRK
QRGHNVETALRRSSVNFVKWSSQGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQGVKLLFLVGGFA
ESAVLQHAVQAALGARGLRVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGRHPPEK
LLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDARFITDPGVRKCGAL
SLELEPADCGQDTAGAPPGRREIRAAMQFGDTEIKVTAVDVSTNRSVRASIDFLSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052970
ORF Size 2058 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052970.5
RefSeq Size 3178 bp
RefSeq ORF 2061 bp
Locus ID 116835
UniProt ID Q96MM6
Cytogenetics 20p13
MW 75.7 kDa
Summary The protein encoded by this gene contains an atypical heat shock protein 70 (Hsp70) ATPase domain and is therefore a distant member of the mammalian Hsp70 family. This gene may be involved in susceptibility to atherosclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_052970" in other vectors (7)
SKU Description Size Price
RC211102L1 Lenti ORF clone of Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1, Myc-DDK-tagged 10 ug
$928.00
RC211102L2 Lenti ORF clone of Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1, mGFP tagged 10 ug
$928.00
RC211102L3 Lenti ORF clone of Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1, Myc-DDK-tagged 10 ug
$928.00
RC211102L4 Lenti ORF clone of Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1, mGFP tagged 10 ug
$928.00
RG211102 HSPA12B (tGFP-tagged) - Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1 10 ug
$789.00 $828.00
SC120181 HSPA12B (untagged)-Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1 10 ug
$629.00
SC324349 HSPA12B (untagged)-Human heat shock 70kD protein 12B (HSPA12B), transcript variant 1 10 ug
$629.00

Plasmid Map for RC211102

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211102
Circular map for RC211102

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products