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PRDM10 (NM_199437) Human Tagged ORF Clone

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SKU
RC211078
PRDM10 (Myc-DDK-tagged)-Human PR domain containing 10 (PRDM10), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,006.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRDM10
Synonyms PFM7; TRIS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211078 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTCCAAAGATGAAAGCTCGCATGTGTGGCCGACATCTGCAGAGCATGAACAGAATGCCGCACAGG
TGCACTTTGTTCCGGACACAGGAACAGTGGCTCAGATTGTCTATACCGATGACCAGGTTCGCCCCTCACA
GCAGGTGGTGTACACGGCAGATGGTGCCTCCTACACATCAGTGGATGGTCCAGAGCACACGCTGGTGTAC
ATCCACCCGGTGGAAGCTGCACAGACTCTGTTTACAGACCCCGGCCAGGTAGCTTATGTCCAACAGGATG
CTACAGCTCAGCAGGCCTCATTGCCAGTTCATAACCAGGTGCTGCCTTCCATCGAGAGTGTAGATGGGTC
CGACCCTTTGGCAACTCTGCAGACCCCTCTAGGCAGACTGGAGGCCAAAGAGGAAGAGGATGAGGATGAG
GACGAGGACACTGAGGAAGATGAGGAAGAAGACGGTGAGGACACGGATCTGGATGACTGGGAGCCAGACC
CGCCCCGGCCCTTCGACCCACACGACTTGTGGTGTGAGGAGTGCAATAACGCGCATGCTTCAGTGTGTCC
GAAGCACGGCCCCTTGCACCCGATCCCCAACCGGCCGGTGCTCACCCGGGCCAGGGCGAGCCTCCCCCTG
GTGCTCTACATAGACAGGTTTCTGGGCGGGGTGTTCTCCAAGCGGCGCATCCCCAAGCGCACCCAGTTTG
GCCCCGTGGAGGGGCCTCTCGTCAGGGGCTCGGAGCTGAAAGACTGTTACATTCACCTCAAGGTTTCTCT
TGATAAAGGGGACAGGAAAGAAAGGGATTTACATGAAGACCTATGGTTTGAGTTGTCTGATGAGACGCTT
TGTAACTGGATGATGTTTGTACGGCCAGCCCAGAATCACCTGGAGCAGAACCTGGTGGCTTACCAGTATG
GCCACCATGTGTATTATACAACCATAAAAAATGTGGAGCCCAAGCAGGAACTGAAGGTGTGGTATGCCGC
ATCCTATGCTGAGTTCGTGAACCAGAAAATTCATGACATTTCTGAGGAAGAAAGGAAAGTTCTTCGAGAG
CAAGAGAAGAATTGGCCCTGCTATGAATGTAACCGCCGATTTATAAGCTCGGAGCAGTTGCAACAGCATC
TCAATTCTCATGATGAGAAACTAGATGTGTTTAGCAGAACAAGAGGCAGAGGAAGGGGACGAGGCAAGAG
GCGATTCGGTCCAGGTCGACGGCCGGGGCGTCCTCCAAAATTTATCCGCCTGGAAATCACCAGCGAAAAT
GGGGAAAAGAGTGACGATGGGACACAGGACTTGCTACATTTTCCCACAAAGGAGCAATTTGATGAGGCTG
AACCAGCCACTCTGAATGGGCTGGATCAACCAGAACAGACCACTATCCCAATCCCTCAGCTGCCACAGGA
AACCCAGTCTTCCCTGGAACATGAACCAGAAACTCACACCCTGCACCTGCAGCCGCAGCATGAAGAGAGC
GTGGTGCCCACCCAGAGCACGCTGACAGCCGACGACATGCGCAGAGCCAAGCGCATCCGAAATGCAGCTC
TTCAGCATCTGTTTATTCGGAAGTCCTTCCGGCCTTTTAAATGCTTGCAGTGTGGGAAGGCCTTCCGGGA
AAAGGACAAACTGGACCAGCACTTACGCTTCCATGGGCGGGAGGGGAACTGCCCACTGACCTGTGATCTC
TGTAACAAGGGCTTCATCAGCAGCACATCCTTGGAGAGCCACATGAAGCTCCACTCAGACCAGAAGACTT
ACTCTTGCATTTTTTGCCCAGAATCCTTTGACCGCCTTGATTTGTTGAAAGATCATGTGGCCATTCATAT
CAATGATGGCTACTTCACCTGCCCAACTTGTAAGAAACGGTTCCCAGATTTTATCCAGGTGAAAAAACAC
GTGCGCAGCTTCCACTCAGAAAAGATCTACCAGTGCACAGAGTGTGACAAGGCCTTCTGTCGCCCCGATA
AACTGCGACTCCACATGCTCCGGCATTCGGACCGCAAAGACTTCCTGTGTTCCACCTGTGGGAAGCAATT
TAAGCGAAAAGACAAACTACGGGAACACATGCAGAGGATGCATAATCCTGAGAGGGAGGCCAAGAAAGCC
GACCGCATCAGCCGCTCCAAGACGTTCAAGCCCCGCATCACGTCCACAGACTACGACAGCTTCACGTTCA
AGTGCCGCCTGTGCATGATGGGCTTCCGGCGGCGCGGCATGCTGGTAAATCACTTATCGAAGAGACACCC
AGACATGAAGATAGAAGAGGTGCCAGAGTTAACTCTACCCATCATAAAACCCAATCGTGATTACTTTTGT
CAGTATTGCGATAAGGTTTATAAAAGTGCCAGCAAGCGCAAAGCCCACATTCTGAAGAACCACCCAGGAG
CAGAGCTCCCACCGAGCATTCGGAAGCTCCGACCCGCTGGTCCTGGAGAGCCAGACCCCATGCTGAGCAC
ACACACCCAGCTGACGGGCACCATCGCCACCCCTCCCGTCTGCTGTCCCCACTGCTCCAAGCAGTACAGC
AGCAAGACCAAGATGGTCCAGCACATTCGAAAGAAGCATCCAGAGTTCGCCCAGCTCTCCAACACCATAC
ACACACCACTGACGACAGCTGTGATCAGTGCCACCCCAGCGGTTTTGACTACAGACAGCGCCACTGGAGA
GACTGTGGTGACGACGGACCTGCTCACCCAAGCAATGACAGAACTGTCCCAGACCTTAACGACAGACTAC
CGAACGCCACAAGGGGATTACCAGAGAATTCAGTACATCCCTGTGTCGCAGTCGGCGTCTGGCCTCCAGC
AGCCTCAGCACATACAGCTGCAAGTGGTTCAAGTGGCCTCGGCCACTTCCCCTCACCAGTCACAGCAGTC
CACTGTGGATGTTGGCCAGCTCCATGATCCTCAGCCCTACCCCCAGCACGCCATCCAGGTGCAGCACATC
CAGGTCAGCGAGCCTACCGCCTCGGCCCCGTCCTCCGCCCAGGTATCTGGGCAGCCGTTGAGTCCCTCAG
CCCAGCAGGCTCAGCAGGGGCTCAGCCCCTCCCACATCCAGGGCAGTTCTTCCACACAGGGGCAGGCTCT
GCAGCAGCAGCAGCAGCAGCAGCAGAATTCCTCTGTGCAGCACACGTACCTGCCCAGTGCTTGGAATTCC
TTCCGTGGCTATTCATCTGAGATTCAAATGATGACGCTTCCTCCGGGTCAGTTTGTGATTACAGACAGTG
GTGTGGCAACTCCAGTTACTACTGGCCAGGTGAAGGCGGTTACTTCGGGTCATTATGTGTTATCAGAAAG
TCAATCAGAATTGGAAGAAAAGCAAACTTCTGCCCTCTCTGGTGGAGTCCAGGTCGAGCCACCTGCACAC
AGTGACTCCCTGGACCCCCAGACCAACAGCCAACAGCAGACCACACAGTACATCATCACCACCACCACCA
ACGGGAACGGAAGCAGCGAAGTGCATATCACCAAACCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211078 protein sequence
Red=Cloning site Green=Tags(s)

MDSKDESSHVWPTSAEHEQNAAQVHFVPDTGTVAQIVYTDDQVRPSQQVVYTADGASYTSVDGPEHTLVY
IHPVEAAQTLFTDPGQVAYVQQDATAQQASLPVHNQVLPSIESVDGSDPLATLQTPLGRLEAKEEEDEDE
DEDTEEDEEEDGEDTDLDDWEPDPPRPFDPHDLWCEECNNAHASVCPKHGPLHPIPNRPVLTRARASLPL
VLYIDRFLGGVFSKRRIPKRTQFGPVEGPLVRGSELKDCYIHLKVSLDKGDRKERDLHEDLWFELSDETL
CNWMMFVRPAQNHLEQNLVAYQYGHHVYYTTIKNVEPKQELKVWYAASYAEFVNQKIHDISEEERKVLRE
QEKNWPCYECNRRFISSEQLQQHLNSHDEKLDVFSRTRGRGRGRGKRRFGPGRRPGRPPKFIRLEITSEN
GEKSDDGTQDLLHFPTKEQFDEAEPATLNGLDQPEQTTIPIPQLPQETQSSLEHEPETHTLHLQPQHEES
VVPTQSTLTADDMRRAKRIRNAALQHLFIRKSFRPFKCLQCGKAFREKDKLDQHLRFHGREGNCPLTCDL
CNKGFISSTSLESHMKLHSDQKTYSCIFCPESFDRLDLLKDHVAIHINDGYFTCPTCKKRFPDFIQVKKH
VRSFHSEKIYQCTECDKAFCRPDKLRLHMLRHSDRKDFLCSTCGKQFKRKDKLREHMQRMHNPEREAKKA
DRISRSKTFKPRITSTDYDSFTFKCRLCMMGFRRRGMLVNHLSKRHPDMKIEEVPELTLPIIKPNRDYFC
QYCDKVYKSASKRKAHILKNHPGAELPPSIRKLRPAGPGEPDPMLSTHTQLTGTIATPPVCCPHCSKQYS
SKTKMVQHIRKKHPEFAQLSNTIHTPLTTAVISATPAVLTTDSATGETVVTTDLLTQAMTELSQTLTTDY
RTPQGDYQRIQYIPVSQSASGLQQPQHIQLQVVQVASATSPHQSQQSTVDVGQLHDPQPYPQHAIQVQHI
QVSEPTASAPSSAQVSGQPLSPSAQQAQQGLSPSHIQGSSSTQGQALQQQQQQQQNSSVQHTYLPSAWNS
FRGYSSEIQMMTLPPGQFVITDSGVATPVTTGQVKAVTSGHYVLSESQSELEEKQTSALSGGVQVEPPAH
SDSLDPQTNSQQQTTQYIITTTTNGNGSSEVHITKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199437
ORF Size 3468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199437.1, NP_955469.1
RefSeq Size 6318 bp
RefSeq ORF 3471 bp
Locus ID 56980
UniProt ID Q9NQV6
Cytogenetics 11q24.3
Protein Families Transcription Factors
MW 130.9 kDa
Summary The protein encoded by this gene is a transcription factor that contains C2H2-type zinc-fingers. It also contains a positive regulatory domain, which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_199437" in other vectors (6)
SKU Description Size Price
RC211078L1 Lenti ORF clone of Human PR domain containing 10 (PRDM10), transcript variant 2, Myc-DDK-tagged 10 ug
$1,306.00
RC211078L2 Lenti ORF clone of Human PR domain containing 10 (PRDM10), transcript variant 2, mGFP tagged 10 ug
$1,306.00
RC211078L3 Lenti ORF clone of Human PR domain containing 10 (PRDM10), transcript variant 2, Myc-DDK-tagged 10 ug
$1,306.00
RC211078L4 Lenti ORF clone of Human PR domain containing 10 (PRDM10), transcript variant 2, mGFP tagged 10 ug
$1,306.00
RG211078 PRDM10 (tGFP-tagged) - Human PR domain containing 10 (PRDM10), transcript variant 2 10 ug
$789.00 $1,206.00
SC307973 PRDM10 (untagged)-Human PR domain containing 10 (PRDM10), transcript variant 2 10 ug
$1,108.00

Plasmid Map for RC211078

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211078
Circular map for RC211078

file-alt Citations

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