TMEM195 (AGMO) (NM_001004320) Human Tagged ORF Clone

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SKU
RC211070
AGMO (Myc-DDK-tagged)-Human alkylglycerol monooxygenase (AGMO)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM195
Synonyms TMEM195
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211070 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAACCCAGAAGCCCAGCAGGATGTTTCAGTTTCCCAGGGATTTCGCATGTTGTTTTACACGATGA
AACCCAGTGAAACTTCATTCCAAACATTAGAAGAGGTGCCTGATTATGTAAAAAAGGCAACTCCATTTTT
CATTTCTTTGATGCTGCTTGAACTTGTTGTCAGCTGGATTCTCAAAGGAAAGCCACCAGGTCGCCTGGAT
GATGCTTTAACGTCAATCTCAGCTGGTGTTCTGTCTCGACTTCCAAGTCTATTTTTCAGGAGCATTGAAC
TGACCAGTTATATTTATATCTGGGAGAACTACAGGCTGTTCAATTTGCCTTGGGATTCTCCATGGACTTG
GTATTCAGCCTTCTTAGGAGTTGACTTTGGCTACTACTGGTTCCATCGTATGGCTCATGAAGTTAATATT
ATGTGGGCCGGACACCAAACACATCATAGTTCTGAAGACTATAACTTATCCACAGCACTGAGACAGTCTG
TCCTCCAGATATATACTTCCTGGATTTTCTACTCTCCCCTGGCCCTCTTCATACCCCCTTCAGTATATGC
TGTTCATCTTCAATTCAATCTTCTTTACCAATTTTGGATCCATACAGAGGTCATCAATAACCTTGGTCCT
TTGGAACTGATTCTTAATACTCCTAGCCATCATAGGGTTCATCATGGCAGAAATCGTTATTGCATAGACA
AAAATTATGCTGGTGTTCTTATTATTTGGGATAAAATTTTTGGGACATTTGAAGCAGAAAATGAAAAAGT
TGTATATGGCTTAACACATCCCATTAATACATTTGAACCAATCAAAGTGCAGTTCCATCACTTATTTTCC
ATATGGACTACATTCTGGGCCACACCTGGATTCTTCAATAAGTTTTCTGTCATATTTAAGGGACCGGGAT
GGGGTCCAGGTAAACCAAGACTTGGTCTCAGTGAAGAAATTCCAGAGGTCACCGGCAAAGAAGTTCCCTT
CTCATCATCTTCATCTCAGCTATTAAAGATATATACAGTTGTACAGTTTGCTCTGATGTTGGCATTTTAT
GAAGAGACCTTTGCAGATACAGCTGCACTGTCGCAAGTTACTCTCCTTCTGAGGGTTTGCTTCATTATCC
TGACCTTGACTTCCATTGGATTTCTTCTGGATCAAAGACCCAAGGCAGCTATTATGGAAACTCTCCGTTG
CTTGATGTTCTTAATGCTGTACCGATTTGGTCACCTGAAGCCTCTTGTCCCTTCATTGTCATCTGCTTTT
GAGATTGTTTTTTCCATTTGCATTGCTTTCTGGGGAGTTAGAAGCATGAAACAACTCACCTCTCACCCTT
GGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211070 protein sequence
Red=Cloning site Green=Tags(s)

MKNPEAQQDVSVSQGFRMLFYTMKPSETSFQTLEEVPDYVKKATPFFISLMLLELVVSWILKGKPPGRLD
DALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLPWDSPWTWYSAFLGVDFGYYWFHRMAHEVNI
MWAGHQTHHSSEDYNLSTALRQSVLQIYTSWIFYSPLALFIPPSVYAVHLQFNLLYQFWIHTEVINNLGP
LELILNTPSHHRVHHGRNRYCIDKNYAGVLIIWDKIFGTFEAENEKVVYGLTHPINTFEPIKVQFHHLFS
IWTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVVQFALMLAFY
EETFADTAALSQVTLLLRVCFIILTLTSIGFLLDQRPKAAIMETLRCLMFLMLYRFGHLKPLVPSLSSAF
EIVFSICIAFWGVRSMKQLTSHPWK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004320
ORF Size 1335 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004320.2
RefSeq Size 2475 bp
RefSeq ORF 1338 bp
Locus ID 392636
UniProt ID Q6ZNB7, X5D773
Cytogenetics 7p21.2
Protein Families Transmembrane
MW 51.5 kDa
Summary The protein encoded by this gene is a tetrahydrobiopterin- and iron-dependent enzyme that cleaves the ether bond of alkylglycerols. Sequence comparisons distinguish this protein as forming a third, distinct class of tetrahydrobiopterin-dependent enzymes. Variations in this gene have been associated with decreased glucose-stimulated insulin response, type 2 diabetes, and susceptibility to intracranial aneurysms. provided by RefSeq, Aug 2012
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Documents
Resources
"NM_001004320" in other vectors (6)
SKU Description Size Price
RC211070L1 Lenti ORF clone of Human alkylglycerol monooxygenase (AGMO), Myc-DDK-tagged 10 ug
$986.00
RC211070L2 Lenti ORF clone of Human alkylglycerol monooxygenase (AGMO), mGFP tagged 10 ug
$986.00
RC211070L3 Lenti ORF clone of Human alkylglycerol monooxygenase (AGMO), Myc-DDK-tagged 10 ug
$986.00
RC211070L4 Lenti ORF clone of Human alkylglycerol monooxygenase (AGMO), mGFP tagged 10 ug
$986.00
RG211070 AGMO (tGFP-tagged) - Human alkylglycerol monooxygenase (AGMO) 10 ug
$886.00
SC300639 AGMO (untagged)-Human alkylglycerol monooxygenase (AGMO) 10 ug
$732.00

Plasmid Map for RC211070

Download sequence for

Cloning scheme for RC211070

Circular map for RC211070

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