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VMAC (NM_001017921) Human Tagged ORF Clone

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SKU
RC211046
VMAC (Myc-DDK-tagged)-Human vimentin-type intermediate filament associated coiled-coil protein (VMAC)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VMAC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCGCCGCCGGCCCTGCAGATCCGGGAGGCAAACGCACACCTGGCAGCCGTGCACCGGCGCGCAG
CGGAGCTGGAGGCGCGGCTGGACGCGGCGGAGCGCACGGTGCACGCCCAAGCCGAGCGCCTGGCCCTCCA
CGACCAGCAGCTGCGCGCCGCCCTAGACGAACTGGGTCGCGCCAAGGACCGTGAGATTGCCACACTCCAG
GAGCAGCTGATGACCTCAGAAGCCACTGTCCACAGCCTGCAGGCCACCGTGCACCAGAGGGACGAGCTCA
TTAGGCAGTTGCAGCCCCGGGCTGAGCTGCTGCAGGACATCTGCCGCCGCCGGCCACCCCTGGCTGGGCT
GCTGGATGCCCTGGCTGAGGCTGAGCGCCTGGGGCCCCTGCCGGCCAGTGACCCCGGCCACCCACCCCCC
GGTGGGCCTGGTCCACCCCTTGACAACAGCACTGGGGAAGAGGCGGACAGGGACCACCTCCAGCCTGCAG
TGTTTGGGACCACAGTG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211046 protein sequence
Red=Cloning site Green=Tags(s)

MSAPPALQIREANAHLAAVHRRAAELEARLDAAERTVHAQAERLALHDQQLRAALDELGRAKDREIATLQ
EQLMTSEATVHSLQATVHQRDELIRQLQPRAELLQDICRRRPPLAGLLDALAEAERLGPLPASDPGHPPP
GGPGPPLDNSTGEEADRDHLQPAVFGTTV

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001017921
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001017921.4
RefSeq Size 1708 bp
RefSeq ORF 510 bp
Locus ID 400673
UniProt ID Q2NL98
Cytogenetics 19p13.3
MW 18.3 kDa
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Documents
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"NM_001017921" in other vectors (4)
SKU Description Size Price
RC211046L3 Lenti ORF clone of Human vimentin-type intermediate filament associated coiled-coil protein (VMAC), Myc-DDK-tagged 10 ug
$600.00
RC211046L4 Lenti ORF clone of Human vimentin-type intermediate filament associated coiled-coil protein (VMAC), mGFP tagged 10 ug
$600.00
RG211046 VMAC (tGFP-tagged) - Human vimentin-type intermediate filament associated coiled-coil protein (VMAC) 10 ug
$500.00
SC322072 VMAC (untagged)-Human vimentin-type intermediate filament associated coiled-coil protein (VMAC) 10 ug
$300.00

Plasmid Map for RC211046

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211046
Circular map for RC211046

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