TREML4 (NM_198153) Human Tagged ORF Clone

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SKU
RC211020
TREML4 (Myc-DDK-tagged)-Human triggering receptor expressed on myeloid cells-like 4 (TREML4)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TREML4
Synonyms TLT-4; TLT4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211020 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGGGGTGGGGTCCACACCTGCTGCTTCCACCTGTGCTGCTGCTGCTCCTGGCCTCAGGGTGCTG
TGCCTGAAGAACTTCACAAACACCCAGGACAGACCCTCCTCCTGCAATGCCAGTACTCACCCAAGAGAGG
GCCCTATCAGCCCAAATCCTGGTGTCAGCAGACATCTCCAAGTCGGTGTACCTTACTTGTCACCAGCTCC
AAGCCCTGGACAGCAGTTCAGAAGTCTCATTACACAATCTGGGACAAGCCCAATGCTGGCTTCTTCAACA
TCACCATGATTCAGCTGACACAGAATGACTCGGGATTCTACTGGTGTGGAATCTACAACGCTTCCGAAAA
CATCATCACTGTTCTTAGAAATATCAGCCTGGTGGTGTCTCCAGCCCCAACCACGTCTCCTATGTGGACT
CTTCCCTGGCTCCCAACAAGCACAGTTCTGATCACTTCTCCAGAGGGGACCTCTGGCCATCCCTCCATCA
ATGGCTCTGAGACCAGGAAATCAAGAGCCCCTGCCTGCCTTGGCTCAGGTGGCCCCAGATTCCTGGTCTT
GGTGCTATGTGGACTCCTCCTGGCCAAGGGCCTGATGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC211020 protein sequence
Red=Cloning site Green=Tags(s)

MAWGGVHTCCFHLCCCCSWPQGAVPEELHKHPGQTLLLQCQYSPKRGPYQPKSWCQQTSPSRCTLLVTSS
KPWTAVQKSHYTIWDKPNAGFFNITMIQLTQNDSGFYWCGIYNASENIITVLRNISLVVSPAPTTSPMWT
LPWLPTSTVLITSPEGTSGHPSINGSETRKSRAPACLGSGGPRFLVLVLCGLLLAKGLML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198153
ORF Size 600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198153.3
RefSeq Size 2088 bp
RefSeq ORF 603 bp
Locus ID 285852
UniProt ID Q6UXN2
Cytogenetics 6p21.1
Protein Families Druggable Genome
MW 21.9 kDa
Summary Positively regulates Toll-like receptor TLR7 signaling in macrophages.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198153" in other vectors (6)
SKU Description Size Price
RC211020L1 Lenti ORF clone of Human triggering receptor expressed on myeloid cells-like 4 (TREML4), Myc-DDK-tagged 10 ug
$600.00
RC211020L2 Lenti ORF clone of Human triggering receptor expressed on myeloid cells-like 4 (TREML4), mGFP tagged 10 ug
$600.00
RC211020L3 Lenti ORF clone of Human triggering receptor expressed on myeloid cells-like 4 (TREML4), Myc-DDK-tagged 10 ug
$600.00
RC211020L4 Lenti ORF clone of Human triggering receptor expressed on myeloid cells-like 4 (TREML4), mGFP tagged 10 ug
$600.00
RG211020 TREML4 (tGFP-tagged) - Human triggering receptor expressed on myeloid cells-like 4 (TREML4) 10 ug
$489.00 $500.00
SC307635 TREML4 (untagged)-Human triggering receptor expressed on myeloid cells-like 4 (TREML4) 10 ug
$330.00

Plasmid Map for RC211020

Download sequence for

Cloning scheme for RC211020

Circular map for RC211020

file-alt Citations

A Longitudinal and Transancestral Analysis of DNA Methylation Patterns in Systemic Lupus Erythematosus
Coit
Thesis (2022)
Snippet: We wanted to explore the role of TREML4 overexpression in neutrophil-like PLB-985 cells receiving TLR7 stimulation. A made-to-order TREML4 open reading frame clone (RC211020;OriGene, Rockville, MD, USA) was inserted into a pCMV6-A-GFP backbone (PS100026;OriGene, Rockville, MD, USA) un
Showing 1-1 of 1 papers.
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