OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SLC28A3 (NM_022127) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC210847
SLC28A3 (Myc-DDK-tagged)-Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $633.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA362117 SLC28A3 Antibody - middle region
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "SLC28A3 or NM_022127"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC28A3
Synonyms CNT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210847 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGAGGAGTACAGCAGCCCCCAGAGCTGAGGGCTACAGCAACGTGGGCTTCCAGAATGAAGAAA
ACTTTCTTGAGAACGAGAACACATCAGGAAACAACTCAATAAGAAGCAGAGCTGTGCAAAGCAGGGAGCA
CACAAACACCAAACAGGATGAAGAACAGGTCACAGTTGAGCAGGATTCTCCAAGAAACAGAGAACACATG
GAGGATGATGATGAGGAGATGCAACAAAAAGGGTGTTTGGAAAGGAGGTATGACACGGTATGTGGTTTCT
GTAGGAAACACAAAACAACTCTTCGGCACATCATCTGGGGCATTTTATTAGCAGGTTATCTGGTTATGGT
GATTTCGGCCTGTGTGCTGAACTTTCACAGAGCCCTTCCTCTTTTTGTGATCACCGTGGCTGCCATCTTC
TTTGTTGTCTGGGATCACCTGATGGCCAAATACGAACATCGAATTGATGAGATGCTGTCTCCTGGCAGAA
GGCTTCTAAACAGCCATTGGTTCTGGCTGAAGTGGGTGATCTGGAGCTCCCTGGTCCTAGCAGTTATTTT
CTGGTTGGCCTTTGACACTGCCAAATTGGGTCAACAGCAGCTGGTGTCCTTCGGTGGGCTCATAATGTAC
ATTGTCCTGTTATTTCTATTCTCCAAGTACCCAACCAGAGTTTACTGGAGACCTGTCTTATGGGGAATCG
GGCTACAGTTTCTTCTTGGGCTCTTGATTCTAAGGACTGACCCTGGATTTATAGCTTTTGATTGGTTGGG
CAGACAAGTTCAGACTTTTCTGGAGTACACAGATGCTGGTGCTTCATTTGTCTTTGGTGAGAAATACAAA
GACCACTTCTTTGCATTTAAGGTCCTGCCGATCGTGGTTTTCTTCAGCACTGTGATGTCCATGCTGTACT
ACCTGGGACTGATGCAGTGGATTATTAGAAAGGTTGGATGGATCATGCTAGTTACTACGGGATCATCTCC
TATTGAATCTGTAGTTGCTTCTGGCAATATATTTGTTGGACAAACGGAGTCTCCACTGCTGGTCCGACCA
TATTTACCTTACATCACCAAGTCTGAACTCCACGCCATCATGACCGCCGGGTTCTCTACCATTGCTGGAA
GCGTGCTAGGTGCATACATTTCTTTTGGGGTTCCATCCTCCCACTTGTTAACAGCGTCAGTTATGTCAGC
ACCTGCGTCATTGGCTGCTGCTAAACTCTTTTGGCCTGAGACAGAAAAACCTAAAATAACCCTCAAGAAT
GCCATGAAAATGGAAAGTGGTGATTCAGGGAATCTTCTAGAAGCTGCAACACAGGGAGCATCCTCCTCCA
TCTCCCTGGTGGCCAACATCGCTGTGAATCTGATTGCCTTCCTGGCCCTGCTGTCTTTTATGAATTCAGC
CCTGTCCTGGTTTGGAAACATGTTTGACTACCCACAGCTGAGTTTTGAGCTAATCTGCTCCTACATCTTC
ATGCCCTTTTCCTTCATGATGGGAGTGGAATGGCAGGACAGCTTTATGGTTGCCAGACTCATAGGTTATA
AGACCTTCTTCAATGAATTTGTGGCTTATGAGCACCTCTCAAAATGGATCCACTTGAGGAAAGAAGGTGG
ACCCAAATTTGTAAACGGTGTGCAGCAATATATATCAATTCGTTCTGAGATAATCGCCACTTACGCTCTC
TGTGGTTTTGCCAATATCGGGTCCCTAGGAATCGTGATCGGCGGACTCACATCCATGGCTCCTTCCAGAA
AGCGTGATATCGCCTCGGGGGCAGTGAGAGCTCTGATTGCGGGGACCGTGGCCTGCTTCATGACAGCCTG
CATCGCAGGCATACTCTCCAGCACTCCTGTGGACATCAACTGCCATCACGTTTTAGAGAATGCCTTCAAC
TCCACTTTCCCTGGAAACACAACCAAGGTGATAGCTTGTTGCCAAAGTCTGTTGAGCAGCACTGTTGCCA
AGGGTCCTGGTGAAGTCATCCCAGGAGGAAACCACAGTCTGTATTCTTTGAAGGGCTGCTGCACATTGTT
GAATCCATCGACCTTTAACTGCAATGGGATCTCTAATACATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210847 protein sequence
Red=Cloning site Green=Tags(s)

MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHM
EDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIF
FVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVIFWLAFDTAKLGQQQLVSFGGLIMY
IVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK
DHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRP
YLPYITKSELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKN
AMKMESGDSGNLLEAATQGASSSISLVANIAVNLIAFLALLSFMNSALSWFGNMFDYPQLSFELICSYIF
MPFSFMMGVEWQDSFMVARLIGYKTFFNEFVAYEHLSKWIHLRKEGGPKFVNGVQQYISIRSEIIATYAL
CGFANIGSLGIVIGGLTSMAPSRKRDIASGAVRALIAGTVACFMTACIAGILSSTPVDINCHHVLENAFN
STFPGNTTKVIACCQSLLSSTVAKGPGEVIPGGNHSLYSLKGCCTLLNPSTFNCNGISNTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022127
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022127.3
RefSeq Size 4573 bp
RefSeq ORF 2076 bp
Locus ID 64078
UniProt ID Q9HAS3
Cytogenetics 9q21.32-q21.33
Domains Nucleoside_tra2
Protein Families Transmembrane
MW 76.9 kDa
Summary Nucleoside transporters, such as SLC28A3, regulate multiple cellular processes, including neurotransmission, vascular tone, adenosine concentration in the vicinity of cell surface receptors, and transport and metabolism of nucleoside drugs. SLC28A3 shows broad specificity for pyrimidine and purine nucleosides (Ritzel et al., 2001 PubMed 11032837).supplied by OMIM, Mar 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_022127" in other vectors (6)
SKU Description Size Price
RC210847L1 Lenti ORF clone of Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2, Myc-DDK-tagged 10 ug
$933.00
RC210847L2 Lenti ORF clone of Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2, mGFP tagged 10 ug
$933.00
RC210847L3 Lenti ORF clone of Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2, Myc-DDK-tagged 10 ug
$933.00
RC210847L4 Lenti ORF clone of Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2, mGFP tagged 10 ug
$933.00
RG210847 SLC28A3 (tGFP-tagged) - Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2 10 ug
$789.00 $833.00
SC112807 SLC28A3 (untagged)-Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2 10 ug
$634.00

Plasmid Map for RC210847

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210847
Circular map for RC210847

file-alt Citations

New age-related hearing loss candidate genes in humans: an ongoing challenge
Di Stazio, Morgan, Brumat et al
Gene (2020) 742, 144561 DOI: 10.1016/j.gene.2020.144561
Cited by 15
AI Summary: OriGene’s pCMV6-Entry vectors containing wild-type and mutant cDNAs of candidate genes (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3, and Strn) were used for transfection and protein expression studies to functionally assess gene variants associated with age-related hearing loss in this paper.
Snippet: The WT and human mutant genes of interest (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3 and Strn) cDNAs were cloned into a pCMV6-Entry vector (by Origene, Rockville, MD), Myc tagged, respectively. Vectors were purified through the PureLink HiPure Plasmid Maxiprep kit (Invitrogen) for transfection and protein expression
AMPK-independent inhibition of human macrophage ER stress response by AICAR
Boß, Newbatt, Gupta et al
Sci Rep (2016) 6, 32111 DOI: 10.1038/srep32111
Cited by 33
AI Summary: In this study, OriGene's SLC28A3 plasmid (RC210847) was used to overexpress the CNT3 nucleoside transporter in human macrophages, enabling the investigation of AICAR uptake mechanisms during ER stress response experiments.
Snippet: 100000 cells plated in 6-well plates were cultured overnight and transfected with 1 μg plasmid coding for GFP (pmaxGFP, Lonza) or SLC28A3 (RC210847, Origene) using JetPRIME transfection reagent (Polyplus-transfection) according to manufacturer's instructions ... Cite SaveSaving
Interactions with selected drug renal transporters and transporter-mediated cytotoxicity in antiviral agents from the group of acyclic nucleoside phosphonates
Mandíková, Volková, Pávek et al
Toxicology (2013) 311 (3), 135-46 DOI: 10.1016/j.tox.2013.07.004
Cited by 16
AI Summary: OriGene products, specifically the CNT3 (SLC28A3) expression plasmid (cat. number: RC210847) and the pCMV6-Entry vector (cat. number: PS100001), were used to facilitate transporter expression in cell-based assays investigating the interaction and cytotoxicity of antiviral acyclic nucleoside phosphonates in this study.
Snippet: number: RC211308;CNT3 (SLC28A3) expression plasmid, transcript variant 2, cat. number: RC210847 and pCMV6-Entry vector, cat. number: PS100001 were obtained from OriGene Technologies (Rockville, USA). 2.2. HeLa
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products