C16orf3 (NM_001214) Human Tagged ORF Clone

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SKU
RC210823
C16orf3 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 3 (C16orf3)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C16orf3
Synonyms MGC132509
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210823 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGTCCTCTGCTGCGGGGCAGGAGTCTCCAGGTCACCCCCATCCCTCCCCACCTGCCTGGACCC
TGAAGAAGCCTTCTGAGTCTGTGGCTCAACGTGCGATGTGCAGTGCAAGGGCCTGCCCCGTAGCCTGCCC
CGTAGGCTGCCCCATAGCCTGCCCCGTAAGCTGCCCCGTAGCCTGCCCCGTAGGCTGCCCCGTAGGCTCC
ATGGCCACTGCCCCACAAGGCCTGTCTCCACAGGAATGGGAAGCGGACAGGGAGACGGGCAGCAGCTCAC
ATGCTGGGACAACGCAGTGTTCAATCCATTCTCCATCCAGCAGCTCCAGACATCTTTCCAGAACACAAAC
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210823 protein sequence
Red=Cloning site Green=Tags(s)

MKLSSAAGQESPGHPHPSPPAWTLKKPSESVAQRAMCSARACPVACPVGCPIACPVSCPVACPVGCPVGS
MATAPQGLSPQEWEADRETGSSSHAGTTQCSIHSPSSSSRHLSRTQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001214
ORF Size 351 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001214.3, NP_001205.3
RefSeq Size 1000 bp
RefSeq ORF 354 bp
Locus ID 750
Cytogenetics 16q24.3
MW 11.9 kDa
Summary This gene encodes a long non-coding RNA (lncRNA) that may function as a tumor suppressor. Mutations in this gene have been identified in human papillary thyroid carcinoma (PTC) patients that abrogate the ability of encoded lncRNA to inhibit cancer cell growth. provided by RefSeq, May 2017
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Documents
Resources
"NM_001214" in other vectors (4)
SKU Description Size Price
RC210823L3 Lenti ORF clone of Human chromosome 16 open reading frame 3 (C16orf3), Myc-DDK-tagged 10 ug
$450.00
RC210823L4 Lenti ORF clone of Human chromosome 16 open reading frame 3 (C16orf3), mGFP tagged 10 ug
$450.00
RG210823 C16orf3 (tGFP-tagged) - Human chromosome 16 open reading frame 3 (C16orf3) 10 ug
$350.00
SC302993 C16orf3 (untagged)-Human chromosome 16 open reading frame 3 (C16orf3) 10 ug
$165.00

Plasmid Map for RC210823

Download sequence for

Cloning scheme for RC210823

Circular map for RC210823

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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