Cystatin C (CST3) (NM_000099) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Cystatin C |
| Synonyms | ARMD11; HEL-S-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000099 |
| ORF Size | 438 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000099.3 |
| RefSeq Size | 929 bp |
| RefSeq ORF | 441 bp |
| Locus ID | 1471 |
| UniProt ID | P01034 |
| Cytogenetics | 20p11.21 |
| Domains | CY |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| MW | 15.8 kDa |
| Summary | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. provided by RefSeq, Nov 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000099" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210730L1 | Lenti ORF clone of Human cystatin C (CST3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210730L2 | Lenti ORF clone of Human cystatin C (CST3), mGFP tagged | 10 ug |
$450.00
|
|
| RC210730L3 | Lenti ORF clone of Human cystatin C (CST3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210730L4 | Lenti ORF clone of Human cystatin C (CST3), mGFP tagged | 10 ug |
$450.00
|
|
| RG210730 | CST3 (tGFP-tagged) - Human cystatin C (CST3) | 10 ug |
$489.00
|
|
| SC110862 | CST3 (untagged)-Human cystatin C (CST3) | 10 ug |
$150.00
|
|
Citations
Cystatin C promotes tau protein phosphorylation and causes microtubule instability by inhibiting intracellular turnover of GSK3β in neurons
Duan, Marcellus, Qin et al
Mol. Cell. Neurosci. (2018) 89, 1-8 DOI: 10.1016/j.mcn.2018.03.009
Duan, Marcellus, Qin et al
Mol. Cell. Neurosci. (2018) 89, 1-8 DOI: 10.1016/j.mcn.2018.03.009
AI Summary: OriGene products, specifically the human CysC cDNA in a pcMV6 vector (Myc-Flag-Cys-pCMV6) and the empty vector pCMV6, were used to overexpress Cystatin C in cell models to investigate its role in tau phosphorylation and microtubule instability in this study.
Snippet: HEK-293 cells were cultured and transfected as described previously (Li and Paudel, 2006). Human CysC cDNA in a pcMV6 vector (Myc-Flag-Cys-pCMV6) and empty vector pCMV6 were obtained from Origene, Rockville, MD
Differential cathepsin responses to inhibitor-induced feedback: E-64 and cystatin C elevate active cathepsin S and suppress active cathepsin L in breast cancer cells
Wilder, Walton, Watson et al
Int. J. Biochem. Cell Biol. (2016) 79, 199-208 DOI: 10.1016/j.biocel.2016.08.030
Wilder, Walton, Watson et al
Int. J. Biochem. Cell Biol. (2016) 79, 199-208 DOI: 10.1016/j.biocel.2016.08.030
Cited by 31
AI Summary: OriGene contributed to this study by providing plasmids containing full-length expression sequences of cystatin C and an empty vector control, which were used to transfect breast cancer cells and assess the cellular effects of cathepsin inhibition.
Snippet: RFP tagged MDA-MB-231 breast cancer cells (Cell Biolabs, Inc.) were transfected with one of the plasmids containing full-length expression sequences of either cystatin C or an empty vector control under control by the CMV promoters (Origene) using Lipofectamine ... Cite SaveSaving
Identification of cystatin SA as a novel inhibitor of acid ceramidase
Eliyahu, Shtraizent, He et al
J. Biol. Chem. (2011) 286 (41), 35624-33 DOI: 10.1074/jbc.M111.260372
Eliyahu, Shtraizent, He et al
J. Biol. Chem. (2011) 286 (41), 35624-33 DOI: 10.1074/jbc.M111.260372
Cited by 1
AI Summary: OriGene products, specifically full-length cDNAs encoding cystatins A, B, C, E/M, and SA in the pCMV vector, were used to study the regulatory effects of cystatin SA on acid ceramidase activity in this research.
Snippet: Full-length cDNAs encoding cystatins A, B, C, E/M, and SA in the pCMV vector were purchased from OriGene Technologies. ... the effects of cysSA siRNA on AC activity and ceramide levels, supporting the conclusion that these changes occurred due to down-regulation of cysSA
Showing 1-3 of 3 papers.
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