TDP43 (TARDBP) (NM_007375) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TDP43 |
| Synonyms | ALS10; TDP-43 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007375 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007375.4 |
| RefSeq Size | 4236 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 23435 |
| UniProt ID | Q13148 |
| Cytogenetics | 1p36.22 |
| Domains | RRM |
| Protein Families | Transcription Factors |
| MW | 44.7 kDa |
| Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007375" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210639L1 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210639L2 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), mGFP tagged | 10 ug |
$757.00
|
|
| RC210639L3 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210639L4 | Lenti ORF clone of Human TAR DNA binding protein (TARDBP), mGFP tagged | 10 ug |
$757.00
|
|
| RG210639 | TARDBP (tGFP-tagged) - Human TAR DNA binding protein (TARDBP) | 10 ug |
$489.00
$657.00
|
|
| SC115570 | TARDBP (untagged)-Human TAR DNA binding protein (TARDBP) | 10 ug |
$686.00
|
|
| SC322765 | TARDBP (untagged)-Human TAR DNA binding protein (TARDBP) | 10 ug |
$686.00
|
|
Citations
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy
Atkinson, Leung, Bender et al
Dis Model Mech (2021)
Atkinson, Leung, Bender et al
Dis Model Mech (2021)
Cited by 14
TDP-43 mutations link Amyotrophic Lateral Sclerosis with R-loop homeostasis and R loop-mediated DNA damage
Giannini, Bayona-Feliu, Sproviero et al
PLoS Genet (2020) 16 (12), e1009260 DOI: 10.1371/journal.pgen.1009260
Giannini, Bayona-Feliu, Sproviero et al
PLoS Genet (2020) 16 (12), e1009260 DOI: 10.1371/journal.pgen.1009260
Cited by 112
AI Summary: OriGene contributed to this study by providing the TDP-43 G294V mutant plasmid (PS100010), which was used to investigate the effects of TDP-43 mutations on R-loop accumulation and genome instability.
Snippet: changed from G to T, to obtain G294V, inserted in PS100010), purchased from OriGene
TDP-43 deficiency links Amyotrophic Lateral Sclerosis with R-loop homeostasis and R loop-mediated DNA damage
Giannini, Sproviero, Bayona-Feliu et al
bioRxiv (2020)
Giannini, Sproviero, Bayona-Feliu et al
bioRxiv (2020)
Cited by 10
Truncation of the TAR DNA-binding protein 43 is not a prerequisite for cytoplasmic relocalization, and is suppressed by caspase inhibition and by introduction of the A90V sequence variant
Wobst, Delsing, Brandon et al
PLoS ONE (2017) 12 (5), e0177181 DOI: 10.1371/journal.pone.0177181
Wobst, Delsing, Brandon et al
PLoS ONE (2017) 12 (5), e0177181 DOI: 10.1371/journal.pone.0177181
AI Summary: OriGene contributed N-terminally myc-tagged wild-type and mutant human TDP-43 (TARDBP) plasmids, which were used in this study to investigate the effects of TDP-43 truncation and the A90V sequence variant on cytoplasmic relocalization and caspase-mediated cleavage.
Snippet: 3:1, for 24 hours. Plasmids harboring N-terminally myc-tagged wild-type or mutant human TDP-43 sequences (TARDBP, wild-type reference sequence NM_007375.3) were purchased from Origene. The presence of the A90V ... All 4 versions Cite SaveSaving
Flow cytometric measurement of the cellular propagation of TDP-43 aggregation
Zeineddine, Whiten, Farrawell et al
Prion (2017)
Zeineddine, Whiten, Farrawell et al
Prion (2017)
AI Summary: OriGene contributed to this study by providing the pCMV6-AC-tGFP expression vector containing TDP-43 WT cDNA, which was used for transient transfection to analyze TDP-43 aggregation and propagation.
Snippet: Cells were maintained in an incubator at 37°C under a humidified atmosphere containing 5% (v/v) CO 2 . Transient Transfections. The pCMV6-AC-tGFP expression vector containing TDP-43 WT cDNA was obtained from Origene. ... All 2 versions Cite SaveSaving
Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro
Wobst, Wesolowski, Chadchankar et al
Front Mol Neurosci (2017) 10, 46 DOI: 10.3389/fnmol.2017.00046
Wobst, Wesolowski, Chadchankar et al
Front Mol Neurosci (2017) 10, 46 DOI: 10.3389/fnmol.2017.00046
AI Summary: OriGene contributed to this study by providing and customizing plasmids, including those with the A90V and M337V TDP-43 mutations, enabling the researchers to investigate the effects of these specific variants on TDP-43 localization and pathology.
Snippet: Materials and Methods. Plasmids. All plasmids were purchased from and customized by Origene. ... Custom mutagenesis to generate the A90V (point mutation c.269C>T) and M337V (point mutation c.1009A>G) TDP-43 amino acid substitutions were carried out by Origene ... All 5 versions Cite SaveSaving
Showing 1-6 of 7 papers.
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