VPS35 (NM_018206) Human Tagged ORF Clone

SKU RC210597

VPS35 (Myc-DDK-tagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: VPS35
• Synonyms: MEM3; PARK17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210597 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATACAGGCTGTGA; AGGTCCAGTCATTCCAAATGAAGAGATGCCTGGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTC; TAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCCATT; TCTGATGAACTGCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATC; TCTACGAACTTGTACAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGT; ATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG; CAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATCTTACCTGATG; AAGGAGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTT; TGCAGAAATGAACAAGCTCTGGGTGCGAATGCAGCATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAA; CGAGAAAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAA; ATGTGGAACGTTACAAACAGATTGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTT; GGCTCAAGAATATCTCATGGAGTGTATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAAT; CCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATAATCATTGCTTTAATTG; ATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCGGATATTAAACTTTTTGATAT; ATTTTCACAGCAGGTGGCTACAGTGATACAGTCTAGACAAGACATGCCTTCAGAGGATGTTGTATCTTTA; CAAGTCTCTCTGATTAATCTTGCCATGAAATGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAG; AAACAACAGTGGAGATATTCAATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGA; ACTCACCAGACTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACAT; TTTCACCCACTCTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGTTGTTATGTGCTTAGTAATG; TTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATAATGAATTTGGTATCCACGTT; GATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGATCCAGAAGATTTTGCTGATGAGCAGAGCCTT; GTGGGCCGCTTCATTCATCTGCTGCGCTCTGAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCAC; GAAAACATTTTGGAGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTA; CCAGCTGGCTTTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATT; TTTTCATTTGCCCACCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCCTTAAGACTTT; TTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAATCATGAGACAGTCGCATATGAATTCAT; GTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCCATCACCTTG; ATCATTGGCACTTTTGAAAGGATGAAGTGCTTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTG; CCCTTGCTGCATCCAAACTTCTAAAGAAACCTGATCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTT; CTGGTCTGGCAGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAGTGCCTA; AAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTTATAGAAATTC; TGAACAGATATATCTATTTTTATGAAAAGGAAAATGATGCGGTAACAATTCAGGTTTTAAACCAGCTTAT; CCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGTGAAGAAACAGAGCAGATTAACAAACATTTT; CATAACACACTGGAGCATTTGCGCTTGCGGCGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTC; TCATCCTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210597 protein sequence; Red=Cloning site Green=Tags(s); ; MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAI; SDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGV; QHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRE; RERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLN; PFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSL; QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH; FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSL; VGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKI; FSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITL; IIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL; KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHF; HNTLEHLRLRRESPESEGPIYEGLIL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018206
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018206.5
• RefSeq Size: 3298 bp
• RefSeq ORF: 2391 bp
• Locus ID: 55737
• UniProt ID: Q96QK1
• Cytogenetics: 16q11.2
• Domains: Vps35
• MW: 91.7 kDa
• Summary: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]