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CGRP (CALCB) (NM_000728) Human Tagged ORF Clone

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SKU
RC210496
CALCB (Myc-DDK-tagged)-Human calcitonin-related polypeptide beta (CALCB)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CGRP or NM_000728"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CGRP
Synonyms CALC2; CGRP-II; CGRP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210496 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTTCCGGAAGTTCTCCCCCTTCCTGGCTCTCAGTATCTTGGTCCTGTACCAGGCGGGCAGCCTCC
AGGCGGCGCCATTCAGGTCTGCCCTGGAGAGCAGCCCAGACCCGGCCACACTCAGTAAAGAGGACGCGCG
CCTCCTGCTGGCTGCACTGGTGCAGGACTATGTGCAGATGAAGGCCAGTGAGCTGAAGCAGGAGCAGGAG
ACACAGGGCTCCAGCTCCGCTGCCCAGAAGAGAGCCTGCAACACTGCCACCTGTGTGACTCATCGGCTGG
CAGGCTTGCTGAGCAGATCAGGGGGCATGGTGAAGAGCAACTTCGTGCCCACCAATGTGGGTTCCAAAGC
CTTTGGCAGGCGCCGCAGGGACCTTCAAGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210496 protein sequence
Red=Cloning site Green=Tags(s)

MGFRKFSPFLALSILVLYQAGSLQAAPFRSALESSPDPATLSKEDARLLLAALVQDYVQMKASELKQEQE
TQGSSSAAQKRACNTATCVTHRLAGLLSRSGGMVKSNFVPTNVGSKAFGRRRRDLQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000728
ORF Size 381 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000728.4
RefSeq Size 1031 bp
RefSeq ORF 384 bp
Locus ID 797
UniProt ID P10092
Cytogenetics 11p15.2
Protein Families Druggable Genome, Secreted Protein
MW 13.7 kDa
Summary CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.UniProtKB/Swiss-Prot Function
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"NM_000728" in other vectors (4)
SKU Description Size Price
RC210496L3 Lenti ORF clone of Human calcitonin-related polypeptide beta (CALCB), Myc-DDK-tagged 10 ug
$450.00
RC210496L4 Lenti ORF clone of Human calcitonin-related polypeptide beta (CALCB), mGFP tagged 10 ug
$450.00
RG210496 CALCB (tGFP-tagged) - Human calcitonin-related polypeptide beta (CALCB) 10 ug
$350.00
SC321719 CALCB (untagged)-Human calcitonin-related polypeptide beta (CALCB) 10 ug
$150.00

Plasmid Map for RC210496

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210496
Circular map for RC210496

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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