SRP1 (KPNA1) (NM_002264) Human Tagged ORF Clone

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SKU
RC210333
KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1
  $751.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SRP1
Synonyms IPOA5; NPI-1; RCH2; SRP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210333 representing NM_002264
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGA
TGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCG
GAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCT
CAGATTAATAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCA
AAAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCC
TATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAA
TTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAGATGTCCAGGA
ACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGC
AATATCCTTCCCCCTCTTTTGCAGTTATTTTCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTAT
GGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAA
TGTGCTTTCCTGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGACTTGTGGAAC
TGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTGGGAAACATTGTCACAGGGGA
TGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTGAGTAGCCCA
AAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCC
AGACTGTGATAGATGCCAACATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAG
AAAAGAAGCAGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTACAGGTTGCCC
TAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCC
TTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAG
GAGATCTACCAAAAGGCCTTTGATCTTATTGAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTG
CACCCCAGGTTGACCTTAACCAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCA
GCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210333 protein sequence
Red=Cloning site Green=Tags(s)

MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEA
QINNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKEN
CTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDC
NILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSY
LSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSP
KESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLV
ELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQ
EIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_002264
ORF Size 1614 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002264.2, NP_002255.2
RefSeq Size 6887 bp
RefSeq ORF 1617 bp
Locus ID 3836
UniProt ID P52294
Cytogenetics 3q21.1
Domains Armadillo_seg, IBB
MW 60 kDa
Summary The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2012
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Documents
Resources
"NM_002264" in other vectors (7)
SKU Description Size Price
RC210333L1 Lenti-ORF clone of KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$1,051.00
RC210333L2 Lenti-ORF clone of KPNA1 (mGFP-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$1,051.00
RC210333L3 Lenti-ORF clone of KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$1,051.00
RC210333L4 Lenti-ORF clone of KPNA1 (mGFP-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$1,051.00
RG210333 KPNA1 (tGFP-tagged) - Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$951.00
SC128103 KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$752.00
SC322070 KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 10 ug
$752.00

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