PHD3 (EGLN3) (NM_022073) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PHD3 |
| Synonyms | HIFP4H3; HIFPH3; PHD3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_022073 |
| ORF Size | 717 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022073.4 |
| RefSeq Size | 2722 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 112399 |
| UniProt ID | Q9H6Z9 |
| Cytogenetics | 14q13.1 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Families | Druggable Genome |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma |
| MW | 27.3 kDa |
| Summary | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022073" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210319L1 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210319L2 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), mGFP tagged | 10 ug |
$600.00
|
|
| RC210319L3 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210319L4 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), mGFP tagged | 10 ug |
$600.00
|
|
| RG210319 | EGLN3 (tGFP-tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3) | 10 ug |
$489.00
$500.00
|
|
| SC112867 | EGLN3 (untagged)-Human egl nine homolog 3 (C. elegans) (EGLN3) | 10 ug |
$300.00
|
|
Citations
ROS- and HIF1α-dependent IGFBP3 upregulation blocks IGF1 survival signaling and thereby mediates high-glucose-induced cardiomyocyte apoptosis
Huang, Liu, Yang et al
J. Cell. Physiol. (2019)
Huang, Liu, Yang et al
J. Cell. Physiol. (2019)
AI Summary: OriGene's pCMV-PHD3 plasmid was used in this study to overexpress PHD3 and investigate its role in the molecular pathway linking oxidative stress, hypoxia, and cardiomyocyte apoptosis under high-glucose conditions.
Snippet: Transfection of siRNA was conducted with transfection reagent. Putative silencing was authenticated by immunoblotting with cellular extracts after transfection. Plasmids with the pCMV‐PHD3 construct were purchased from OriGene Technologies, Inc. (Rockville, MD
Restoration of the prolyl-hydroxylase domain protein-3 oxygen-sensing mechanism is responsible for regulation of HIF2α expression and induction of sensitivity of myeloma cells to hypoxia-mediated apoptosis
Gastelum, Poteshkina, Veena et al
PLoS ONE (2017) 12 (12), e0188438 DOI: 10.1371/journal.pone.0188438
Gastelum, Poteshkina, Veena et al
PLoS ONE (2017) 12 (12), e0188438 DOI: 10.1371/journal.pone.0188438
AI Summary: In this study, the PHD3/EGLN3 open reading frame (ORF) lentivirus vector from OriGene was used to restore PHD3 expression in myeloma cells, enabling the researchers to investigate its role in regulating HIF2α expression and sensitivity to hypoxia-mediated apoptosis.
Snippet: Littleton, CO). Generation of PHD3 isogenic 8226 cell lines. The PHD3/EGLN3 open reading frame (ORF) (ref sequence NM_022073.3) was cloned into a lentivirus vector purchased from OriGene (Rockville MD). Isogenic 8226 Cached
Showing 1-2 of 2 papers.
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