TMEM198 (NM_001005209) Human Tagged ORF Clone

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SKU
RC210300
TMEM198 (Myc-DDK-tagged)-Human transmembrane protein 198 (TMEM198)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM198
Synonyms TMEM198A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGGACTGTGGCAACACTGCGGTTCCAGCTGCTGCCCCCTGAGCCAGATGATGCCTTCTGGGGTG
CACCTTGTGAACAGCCCCTGGAGCGCAGGTACCAGGCACTGCCGGCCCTCGTCTGCATCATGTGCTGTTT
GTTTGGAGTCGTCTACTGCTTCTTCGGTTACCGCTGCTTCAAGGCAGTGCTCTTCCTCACTGGGTTGCTG
TTTGGCTCGGTGGTCATCTTCCTCCTCTGCTACCGAGAGCGGGTGCTAGAGACACAGCTGAGTGCTGGGG
CGAGCGCGGGCATCGCTCTGGGCATCGGGCTGCTCTGCGGGCTGGTGGCCATGCTAGTGCGCAGCGTGGG
CCTCTTCCTGGTGGGGCTGCTGCTCGGCCTGCTGCTCGCAGCTGCTGCCCTGCTGGGCTCCGCACCCTAC
TACCAGCCAGGCTCCGTGTGGGGTCCACTGGGGCTGTTGCTGGGGGGCGGCCTGCTCTGTGCCCTGCTCA
CTCTGCGCTGGCCCCGCCCACTCACCACCCTGGCCACCGCCGTGACTGGTGCTGCGCTGATCGCCACTGC
CGCTGACTACTTCGCCGAGCTGCTACTGCTGGGGCGCTACGTGGTGGAGCGACTCCGGGCTGCTCCTGTG
CCCCCACTCTGCTGGCGAAGCTGGGCCCTGCTGGCACTCTGGCCCCTGCTCAGCCTGATGGGCGTTCTGG
TGCAGTGGAGGGTGACAGCTGAGGGGGACTCCCACACGGAAGTGGTCATCAGCCGGCAGCGCCGACGCGT
GCAACTGATGCGGATTCGGCAGCAGGAAGATCGCAAGGAGAAAAGGCGGAAAAAGAGACCTCCTCGGGCT
CCCCTCAGAGGTCCCCGGGCTCCTCCCAGGCCTGGGCCACCAGATCCTGCTTATCGGCGCAGGCCAGTGC
CCATCAAACGCTTCAATGGAGACGTCCTCTCCCCGAGCTATATCCAGAGCTTCCGAGACCGGCAGACCGG
GAGCTCCCTGAGCTCCTTCATGGCCTCACCCACAGATGCGGACTATGAGTATGGGTCCCGGGGACCTCTG
ACAGCCTGCTCAGGCCCCCCAGTGCGGGTA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210300 protein sequence
Red=Cloning site Green=Tags(s)

MPGTVATLRFQLLPPEPDDAFWGAPCEQPLERRYQALPALVCIMCCLFGVVYCFFGYRCFKAVLFLTGLL
FGSVVIFLLCYRERVLETQLSAGASAGIALGIGLLCGLVAMLVRSVGLFLVGLLLGLLLAAAALLGSAPY
YQPGSVWGPLGLLLGGGLLCALLTLRWPRPLTTLATAVTGAALIATAADYFAELLLLGRYVVERLRAAPV
PPLCWRSWALLALWPLLSLMGVLVQWRVTAEGDSHTEVVISRQRRRVQLMRIRQQEDRKEKRRKKRPPRA
PLRGPRAPPRPGPPDPAYRRRPVPIKRFNGDVLSPSYIQSFRDRQTGSSLSSFMASPTDADYEYGSRGPL
TACSGPPVRV

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005209
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005209.2, NP_001005209.1
RefSeq Size 2239 bp
RefSeq ORF 1083 bp
Locus ID 130612
UniProt ID Q66K66, A0A024R483
Cytogenetics 2q35
Protein Families Transmembrane
MW 39.5 kDa
Summary Promotes LRP6 phosphorylation by casein kinases and thereby plays a role in Wnt signaling. May be a membrane scaffold protein involved in the self-aggregation of LRP6 to further enhance its activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001005209" in other vectors (4)
SKU Description Size Price
RC210300L3 Lenti ORF clone of Human transmembrane protein 198 (TMEM198), Myc-DDK-tagged 10 ug
$757.00
RC210300L4 Lenti ORF clone of Human transmembrane protein 198 (TMEM198), mGFP tagged 10 ug
$757.00
RG210300 TMEM198 (tGFP-tagged) - Human transmembrane protein 198 (TMEM198) 10 ug
$657.00
SC300847 TMEM198 (untagged)-Human transmembrane protein 198 (TMEM198) 10 ug
$503.00

Plasmid Map for RC210300

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Cloning scheme for RC210300

Circular map for RC210300

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