WDR8 (WRAP73) (NM_017818) Human Tagged ORF Clone

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SKU
RC210272
WRAP73 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP73 (WRAP73)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR8
Synonyms WDR8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210272 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGCAAGTACCTGG
CTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAGATCCTTCAGCTGTACACGTG
CCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCGCTCTTCATCCTGTGCGCCATGTACAAGCGA
GGGCTGGTGCAGGTCTGGTCTTTAGAGCAGCCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGC
TGGTGGCCTCGTGCTGGAGCCCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAAC
CGTCTGGTCCTTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC
TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTGAGCATCTTCG
TCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGATCTCACAGGGATTGAGTGGGC
CCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTGGAGTACAAGATTCTGCTGTACTCATTGGAT
GGCCGGTTGTTGTCCACGTACAGCGCTTACGAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCA
GCAGTCAGTTCCTGGCAGTTGGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAAT
GATCACGGAGTTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG
AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCTCTCCCGAGCT
CAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGC
AAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCTGACAGCTACTTCCTGGCGACAAGGAACGAC
AACATTCCCAATGCCGTCTGGGTCTGGGACATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGT
CCCCAGTGCGCGCATTTCAGTGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCT
CTACCTGTGGTCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT
CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTCTGCTTCCTGG
AGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210272 protein sequence
Red=Cloning site Green=Tags(s)

MNFSEVFKLSSLLCKFSPDGKYLASCVQYRLVVRDVNTLQILQLYTCLDQIQHIEWSADSLFILCAMYKR
GLVQVWSLEQPEWHCKIDEGSAGLVASCWSPDGRHILNTTEFHLRITVWSLCTKSVSYIKYPKACLQGIT
FTRDGRYMALAERRDCKDYVSIFVCSDWQLLRHFDTDTQDLTGIEWAPNGCVLAVWDTCLEYKILLYSLD
GRLLSTYSAYEWSLGIKSVAWSPSSQFLAVGSYDGKVRILNHVTWKMITEFGHPAAINDPKIVVYKEAEK
SPQLGLGCLSFPPPRAGAGPLPSSESKYEIASVPVSLQTLKPVTDRANPKIGIGMLAFSPDSYFLATRND
NIPNAVWVWDIQKLRLFAVLEQLSPVRAFQWDPQQPRLAICTGGSRLYLWSPAGCMSVQVPGEGDFAVLS
LCWHLSGDSMALLSKDHFCLCFLETEAVVGTACRQLGGHT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017818
ORF Size 1380 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017818.4
RefSeq Size 1708 bp
RefSeq ORF 1383 bp
Locus ID 49856
UniProt ID Q9P2S5, A0A384MQZ3
Cytogenetics 1p36.32
Domains WD40
Protein Families Stem cell - Pluripotency
MW 51.6 kDa
Summary This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Studies of the related mouse protein suggest that the encoded protein may play a role in the process of ossification. provided by RefSeq, Mar 2009
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Documents
Resources
"NM_017818" in other vectors (4)
SKU Description Size Price
RC210272L3 Lenti ORF clone of Human WD repeat containing, antisense to TP73 (WRAP73), Myc-DDK-tagged 10 ug
$757.00
RC210272L4 Lenti ORF clone of Human WD repeat containing, antisense to TP73 (WRAP73), mGFP tagged 10 ug
$757.00
RG210272 WRAP73 (tGFP-tagged) - Human WD repeat containing, antisense to TP73 (WRAP73) 10 ug
$657.00
SC113971 WRAP73 (untagged)-Human WD repeat containing, antisense to TP73 (WRAP73) 10 ug
$457.00

Plasmid Map for RC210272

Download sequence for

Cloning scheme for RC210272

Circular map for RC210272

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