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COQ10A (NM_144576) Human Tagged ORF Clone

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SKU
RC210099
COQ10A (Myc-DDK-tagged)-Human coenzyme Q10 homolog A (S. cerevisiae) (COQ10A), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COQ10A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210099 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGGGCGGGCTCGCGGCGGGTCCCAGCTGGGACGCGCGCGGCAGCCGAGCGCTGCTGCCGGCTCT
CGCTCAGCCCGGGCGCGCAACCGGCCCCGCCCCCAGGCCCTCTGCCACCGCCGCGACCAATGAGGTTTCT
GACCTCCTGCAGCCTCCTCTTGCCTCGGGCTGCCCAGATCTTGGCGGCTGAGGCTGGCTTACCTTCGAGC
CGTTCCTTCATGGGATTTGCTGCTCCCTTCACCAACAAGCGAAAGGCTTACTCGGAGCGTAGAATCATGG
GGTACTCAATGCAGGAGATGTATGAGGTGGTGTCCAACGTCCAGGAGTATCGTGAGTTTGTGCCCTGGTG
TAAGAAGTCTCTGGTGGTATCCAGCCGTAAGGGTCATTTGAAAGCCCAGCTGGAGGTTGGCTTTCCACCT
GTCATGGAACGTTACACCTCTGCAGTTTCCATGGTCAAACCTCACATGGTCAAGGCTGTTTGTACTGATG
GCAAGCTCTTCAACCACTTAGAGACTATTTGGCGATTCAGCCCTGGTATTCCTGCCTATCCTCGAACCTG
CACTGTGGACTTTTCGATTTCCTTTGAATTTCGTTCTCTGCTGCACTCCCAGCTGGCCACCATGTTTTTT
GATGAGGTTGTCAAACAGAATGTTGCTGCCTTTGAGCGTCGGGCAGCCACCAAGTTTGGTCCAGAAACAG
CCATCCCCCGTGAACTGATGTTCCATGAGGTGCACCAGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210099 protein sequence
Red=Cloning site Green=Tags(s)

MAWAGSRRVPAGTRAAAERCCRLSLSPGAQPAPPPGPLPPPRPMRFLTSCSLLLPRAAQILAAEAGLPSS
RSFMGFAAPFTNKRKAYSERRIMGYSMQEMYEVVSNVQEYREFVPWCKKSLVVSSRKGHLKAQLEVGFPP
VMERYTSAVSMVKPHMVKAVCTDGKLFNHLETIWRFSPGIPAYPRTCTVDFSISFEFRSLLHSQLATMFF
DEVVKQNVAAFERRAATKFGPETAIPRELMFHEVHQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144576
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144576.4
RefSeq Size 1674 bp
RefSeq ORF 744 bp
Locus ID 93058
UniProt ID Q96MF6
Cytogenetics 12q13.3
Domains ARPF
MW 27.7 kDa
Summary Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (Probable).UniProtKB/Swiss-Prot Function
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Documents
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"NM_144576" in other vectors (4)
SKU Description Size Price
RC210099L3 Lenti ORF clone of Human coenzyme Q10 homolog A (S. cerevisiae) (COQ10A), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210099L4 Lenti ORF clone of Human coenzyme Q10 homolog A (S. cerevisiae) (COQ10A), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$600.00
RG210099 COQ10A (tGFP-tagged) - Human coenzyme Q10 homolog A (S. cerevisiae) (COQ10A), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$500.00
SC322755 COQ10A (untagged)-Human coenzyme Q10 homolog A (S. cerevisiae) (COQ10A), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$300.00

Plasmid Map for RC210099

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210099
Circular map for RC210099

file-alt Citations

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