FBXL3 (NM_012158) Human Tagged ORF Clone

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SKU
RC210074
FBXL3 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL3
Synonyms FBL3; FBL3A; FBXL3A; IDDSFAS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210074 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACGAGGAGGAAGAGATAGTGACCGTAATTCATCAGAAGAAGGAACTGCAGAGAAATCCAAGAAAC
TGAGGACTACAAATGAGCATTCTCAGACTTGTGATTGGGGTAATCTCCTTCAGGACATTATTCTCCAAGT
ATTTAAATATTTGCCTCTTCTTGACCGGGCTCATGCTTCACAAGTTTGCCGCAACTGGAACCAGGTATTT
CACATGCCTGACTTGTGGAGATGTTTTGAATTTGAACTGAATCAGCCAGCTACATCTTATTTGAAAGCTA
CCCATCCAGAGCTGATCAAACAGATTATTAAAAGACATTCAAACCATCTACAATATGTCAGCTTCAAGGT
GGACAGCAGCAAGGAATCAGCTGAAGCAGCTTGTGATATACTATCGCAACTTGTGAATTGCTCTTTAAAA
ACACTTGGACTTATTTCAACTGCTCGACCAAGCTTTATGGATTTACCAAAGTCTCACTTTATCTCTGCAC
TGACAGTTGTGTTCGTAAACTCCAAATCCCTGTCTTCGCTTAAGATAGATGATACTCCAGTAGATGATCC
ATCTCTCAAAGTACTAGTGGCCAACAATAGTGATACACTCAAGCTGTTGAAAATGAGCAGCTGTCCTCAT
GTCTCTCCAGCAGGTATCCTTTGTGTGGCTGATCAGTGTCACGGCTTAAGAGAACTAGCCCTGAACTACC
ACTTATTGAGTGATGAGTTGTTACTTGCATTGTCTTCTGAAAAACATGTTCGATTAGAACATTTGCGCAT
TGATGTAGTCAGTGAGAATCCTGGACAGACACACTTCCATACTATTCAGAAGAGTAGCTGGGATGCTTTC
ATCAGACATTCACCCAAAGTGAACTTAGTGATGTATTTTTTTTTATATGAAGAAGAATTTGACCCCTTCT
TTCGCTATGAAATACCTGCCACCCATCTGTACTTTGGGAGATCAGTAAGCAAAGATGTGCTTGGCCGTGT
GGGAATGACATGCCCTAGACTGGTTGAACTAGTAGTGTGTGCAAATGGATTACGGCCACTTGATGAAGAG
TTAATTCGCATTGCAGAACGTTGCAAAAATTTGTCAGCTATTGGACTAGGGGAATGTGAAGTCTCATGTA
GTGCCTTTGTTGAGTTTGTGAAGATGTGTGGTGGCCGCCTATCTCAATTATCCATTATGGAAGAAGTACT
AATTCCTGACCAAAAGTATAGTTTGGAGCAGATTCACTGGGAAGTGTCCAAGCATCTTGGTAGGGTGTGG
TTTCCCGACATGATGCCCACTTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210074 protein sequence
Red=Cloning site Green=Tags(s)

MKRGGRDSDRNSSEEGTAEKSKKLRTTNEHSQTCDWGNLLQDIILQVFKYLPLLDRAHASQVCRNWNQVF
HMPDLWRCFEFELNQPATSYLKATHPELIKQIIKRHSNHLQYVSFKVDSSKESAEAACDILSQLVNCSLK
TLGLISTARPSFMDLPKSHFISALTVVFVNSKSLSSLKIDDTPVDDPSLKVLVANNSDTLKLLKMSSCPH
VSPAGILCVADQCHGLRELALNYHLLSDELLLALSSEKHVRLEHLRIDVVSENPGQTHFHTIQKSSWDAF
IRHSPKVNLVMYFFLYEEEFDPFFRYEIPATHLYFGRSVSKDVLGRVGMTCPRLVELVVCANGLRPLDEE
LIRIAERCKNLSAIGLGECEVSCSAFVEFVKMCGGRLSQLSIMEEVLIPDQKYSLEQIHWEVSKHLGRVW
FPDMMPTW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012158
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012158.4
RefSeq Size 3572 bp
RefSeq ORF 1287 bp
Locus ID 26224
UniProt ID Q9UKT7
Cytogenetics 13q22.3
Domains F-box
Protein Families Druggable Genome
MW 48.7 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_012158" in other vectors (7)
SKU Description Size Price
RC210074L1 Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), Myc-DDK-tagged 10 ug
$757.00
RC210074L2 Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), mGFP tagged 10 ug
$757.00
RC210074L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), Myc-DDK-tagged 10 ug
$757.00
RC210074L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 3 (FBXL3), mGFP tagged 10 ug
$757.00
RG210074 FBXL3 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 3 (FBXL3) 10 ug
$489.00 $657.00
SC111230 FBXL3 (untagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3) 10 ug
$457.00
SC324339 FBXL3 (untagged)-Human F-box and leucine-rich repeat protein 3 (FBXL3) 10 ug
$457.00

Plasmid Map for RC210074

Download sequence for

Cloning scheme for RC210074

Circular map for RC210074

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