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TOMM7 (NM_019059) Human Tagged ORF Clone

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SKU
RC210040
TOMM7 (Myc-DDK-tagged)-Human translocase of outer mitochondrial membrane 7 homolog (yeast) (TOMM7), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TOMM7 or NM_019059"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TOMM7
Synonyms TOM7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210040 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGCTGAGCAAAGAGGCCAAGCAGAGACTACAGCAGCTCTTCAAGGGGAGCCAGTTTGCCATTC
GCTGGGGCTTTATCCCTCTTGTGATTTACCTGGGATTTAAGAGGGGTGCAGATCCCGGAATGCCTGAACC
AACTGTTTTGAGCCTACTTTGGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210040 protein sequence
Red=Cloning site Green=Tags(s)

MVKLSKEAKQRLQQLFKGSQFAIRWGFIPLVIYLGFKRGADPGMPEPTVLSLLWG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019059
ORF Size 165 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019059.5
RefSeq Size 796 bp
RefSeq ORF 168 bp
Locus ID 54543
UniProt ID Q9P0U1
Cytogenetics 7p15.3
Protein Families Transmembrane
MW 6.2 kDa
Summary This gene encodes a subunit of the translocase of the outer mitochondrial membrane. The encoded protein regulates the assembly and stability of the translocase complex. provided by RefSeq, Oct 2012
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"NM_019059" in other vectors (4)
SKU Description Size Price
RC210040L3 Lenti ORF clone of Human translocase of outer mitochondrial membrane 7 homolog (yeast) (TOMM7), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC210040L4 Lenti ORF clone of Human translocase of outer mitochondrial membrane 7 homolog (yeast) (TOMM7), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG210040 TOMM7 (tGFP-tagged) - Human translocase of outer mitochondrial membrane 7 homolog (yeast) (TOMM7), nuclear gene encoding mitochondrial protein 10 ug
$489.00
SC113263 TOMM7 (untagged)-Human translocase of outer mitochondrial membrane 7 homolog (yeast) (TOMM7), nuclear gene encoding mitochondrial protein 10 ug
$150.00

Plasmid Map for RC210040

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210040
Circular map for RC210040

file-alt Citations

A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy
Zeb, Choubey, Gupta et al
Redox Biol (2021) 48, 102186 DOI: 10.1016/j.redox.2021.102186
Cited by 93
AI Summary: OriGene contributed PGAM5 shRNA (Cat# TR702898, TI711593) to this study, which was used to investigate the role of PGAM5 in the KEAP1/PGAM5 complex and its involvement in mitophagy regulation.
Snippet: GC TAG Copenhagen A/S rKEAP1-r-ATCTCACACTTCTGCAGCTG Recombinant DNA KEAP1 shRNA SABiosciences Cat# KR47610 N AAGGTCATGGAGAGGCTAATT (no 2) GGACTACCTGGTGCAGATCTT (no 3) PGAM5 shRNA OriGene Cat# TR702898 (TI711593) ATTGAGGCTGCCTTCAGGAACTACATCCA PINK1 shRNA SABiosciences Cat# KR55105 N CTGCAATGCCGCTGTGTATGA DRP1 shRNA SABiosciences Cat# KR45077 N CGAGCAGGGTGT
FigureFigureFigure
Showing 1-1 of 1 papers.
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