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CCDC76 (TRMT13) (NM_019083) Human Tagged ORF Clone

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SKU
RC209930
TRMT13 (Myc-DDK-tagged)-Human coiled-coil domain containing 76 (CCDC76)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC76
Synonyms CCDC76
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209930 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCTCCGCGACGTCGCCGCACGCGCCTGGTTTTCCAGCTGAGGGTAGATGCGGTTACTATGTGG
AAAAGAAGAAACGGTTCTGCAGGATGGTGGTGGCCGCAGGGAAAAGATTTTGTGGTGAACACGCTGGAGC
CATGGAGGAAGAAGATGCTCGGAAAAGAATCCTGTGTCCTTTAGATCCAAAACACACAGTATATGAAGAT
CAACTAGCAAAGCATTTGAAAAAATGTAACTCAAGAGAGAAACCAAAACCTGATTTCTATATTCAAGATA
TTAATGCAGGCTTAAGAGATGAAACAGAAATACCTGAACAATTAGTTCCAATTTCTTCTCTATCTGAAGA
GCAGTTGGAAAAGTTAATTAAGAAATTGAGAAAAGCAAGTGAAGGCTTGAATTCTACACTTAAAGATCAT
ATTATGTCCCATCCAGCATTACACGATGCACTTAATGACCCTAAAAATGGCGATTCTGCAACCAAGCACC
TGAAACAGCAGGCTTCTATTTTAGGTAACATTGAAAATTTAAAGTTACTTGGTCCAAGAAGATGCTTTGT
TGAGTTTGGAGCGGGAAAGGGAAAATTATCTCATTGGGTTGATATTGCCTTAAAAGATGCTGAAAAAGTT
CACTTCATCCTAGTGGAAAAGGTGACCACAAGATTCAAGGTGGATGGAAAACACAGAAAGAAAAATTCAG
TGTTTGAAAGACTTCAAATTGATATTCAACACTTGTGTTTGAACAAGATTCCTGTGCTAAGAGAAGAAAA
ACTACCTGTGGTAGGAATTGGAAAGCATCTGTGTGGTATGGCAACAGATCTTGCATTACGATGTTTGGTT
GAAACCTATGCTGCCAGTTTTGAGGAAAGGAATGAAGAACCTTTAGCCAAACGCATAAAGAATGATAAAA
CAGAAAAAGAAATTTACACTTTGGCCAAGGAAGGAAATGAAAAAAATGTCCCAGAGAAGTGGAACCCTGT
GGCTGGCATTGTTATTGCACTCTGTTGTCACCACAGGTGTGATTGGAGACATTATGTGGGCAAAGAATAT
TTCAGGGCTCTAGGCCTTGGAGCAGTGGAATTCCATTATTTCCAGCGAATGAGTAGTTGGGCAACTTGTG
GGATGCGGAAAACATCTTTGGAAACCTCAAATAGTACCACAAAGAGGCAAGATAATCAGAATGATGATAG
TGAAGAGCATGATGATGGAGGATACAGAATCACAGATGATGGCGCTGATTGTTTGCCTGGGCTTCTTAGT
GTTGAAGAAAAGAAGAAAATAGGGCATCTTTGTAAATTGCTGATTGACCAAGGTCGAATCCAGTATTTGC
AGCAGAAGGGATTCAGTCCTGCTTTGCAGTACTATACAGACCCTCTGGTGTCTTTGGAAAATGTTTTGTT
AACTGCTTTACCAAATCATTCTTCATCACCAGAAACAACTGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209930 protein sequence
Red=Cloning site Green=Tags(s)

MATSATSPHAPGFPAEGRCGYYVEKKKRFCRMVVAAGKRFCGEHAGAMEEEDARKRILCPLDPKHTVYED
QLAKHLKKCNSREKPKPDFYIQDINAGLRDETEIPEQLVPISSLSEEQLEKLIKKLRKASEGLNSTLKDH
IMSHPALHDALNDPKNGDSATKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKV
HFILVEKVTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLV
ETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEY
FRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLS
VEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019083
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019083.1, NP_061956.1
RefSeq Size 3157 bp
RefSeq ORF 1446 bp
Locus ID 54482
UniProt ID Q9NUP7
Cytogenetics 1p21.2
Domains DUF715
MW 54.3 kDa
Summary tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019083" in other vectors (4)
SKU Description Size Price
RC209930L3 Lenti ORF clone of Human coiled-coil domain containing 76 (CCDC76), Myc-DDK-tagged 10 ug
$757.00
RC209930L4 Lenti ORF clone of Human coiled-coil domain containing 76 (CCDC76), mGFP tagged 10 ug
$757.00
RG209930 TRMT13 (tGFP-tagged) - Human coiled-coil domain containing 76 (CCDC76) 10 ug
$657.00
SC113242 TRMT13 (untagged)-Human coiled-coil domain containing 76 (CCDC76) 10 ug
$457.00

Plasmid Map for RC209930

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209930
Circular map for RC209930

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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