FLJ43980 (NM_001004299) Human Tagged ORF Clone

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SKU
RC209893
FLJ43980 (Myc-DDK-tagged)-Human FLJ43980 protein (FLJ43980)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLJ43980
Synonyms MGC87661, DKFZp781D1722
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209893 representing NM_001004299
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGAAGCTCTGGCTTCGGGGGCCAGAAGGGCCAGGGGCCCTCCTGCTCCTTCACCGGCTGTTGGT
GTTGCAGGGGGGACGATGTCGCCGAGAGCGATGACAGCCCCTTCGCCCAGTGCGGGTACAATATCCAAGA
GAAACACCTCGGCAAGCTCCACAGAGCTGCCAGCCGGGGTGAAGTCTCCAAGGTGGAGTGCATCCTTTCG
AGCGGGAGCGCTGACTTGGATGAGAGAGACAAGAAGAAAAGGACTGCTCTGCATTTGGCCTGTGCCAACG
GCCATCCAGAAGTGGTAGCTCTTCTGGTGGACAGAGGATGTCAACTTGATGTCTTTGACAACAAAAACAG
GACTGCTCTGCTAAAGGCGGTACAATGCCAGGAAGAGGAATGTGCGACTATTTTGCTAGAACACGGAGCC
GACCCAGACCTTCCAGATGTCTATGGCAATACTACCTTACACTATGCCATCTACAATGAGGATATCCCAA
TGACAAAAAAACTGCTTTTACACCATGCCAATATTGAATCAGCAAACAAGGATGAACTCACACCATTTTT
GCTAGCTGTACATGAGCAAAAACAGCAAATGGAAGACTTTTTAAGAAAGCAAAAAGAAAATTTAACTGCA
GTTAAGCTTGAAAGCATTCACCAAGTAATGTCTGAATACAAAGAAAATGAGACACCTAGAAATCCTCAAA
ATAGCAATCCAGAAGGAACATCTAACAAGATGGCGTGCTTGGGAGAAGGAGCAGCAGGTGCAAAAGTGGA
TGAGATTCCTGGAAATCCTGTAACAAGGCTTTTCAACAAACCATCTATTGACGACTCACGACCTATGTCA
GCTAATGAAGACTTTGATTTTGATACTGAGGAGAAAGCAACAGAGCCAGCAAATGGAAAAAGACAAAATG
GTATGGGTATTATTGAGAGTGCTCCACAAGAGCACACAAATAATGAAAACATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209893 representing NM_001004299
Red=Cloning site Green=Tags(s)

MRRSSGFGGQKGQGPSCSFTGCWCCRGDDVAESDDSPFAQCGYNIQEKHLGKLHRAASRGEVSKVECILS
SGSADLDERDKKKRTALHLACANGHPEVVALLVDRGCQLDVFDNKNRTALLKAVQCQEEECATILLEHGA
DPDLPDVYGNTTLHYAIYNEDIPMTKKLLLHHANIESANKDELTPFLLAVHEQKQQMEDFLRKQKENLTA
VKLESIHQVMSEYKENETPRNPQNSNPEGTSNKMACLGEGAAGAKVDEIPGNPVTRLFNKPSIDDSRPMS
ANEDFDFDTEEKATEPANGKRQNGMGIIESAPQEHTNNENI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004299
ORF Size 963 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004299.1, NP_001004299.1
RefSeq Size 3722 bp
RefSeq ORF 965 bp
Locus ID 124149
Cytogenetics 16q11.2
MW 35.9 kDa
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Documents
Resources
"NM_001004299" in other vectors (3)
SKU Description Size Price
RC209893L3 Lenti ORF clone of Human FLJ43980 protein (FLJ43980), Myc-DDK-tagged 10 ug
$600.00
RC209893L4 Lenti ORF clone of Human FLJ43980 protein (FLJ43980), mGFP tagged 10 ug
$600.00
RG209893 FLJ43980 (tGFP-tagged) - Human FLJ43980 protein (FLJ43980) 10 ug
$489.00 $500.00

Plasmid Map for RC209893

Download sequence for

Cloning scheme for RC209893

Circular map for RC209893

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