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Cripto1 (TDGF1) (NM_003212) Human Tagged ORF Clone

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SKU
RC209841
TDGF1 (Myc-DDK-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Cripto1
Synonyms CR; CR-1; CRGF; CRIPTO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209841 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGCAGGAAGATGGCCCGCTTCTCTTACAGTGTGATTTGGATCATGGCCATTTCTAAAGCCTTTG
AACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCATCTCGGGGATACCTGGCCTTCAG
AGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTCGGCCTCGGTCTTCCCAGCGTGTGCCGCCCATG
GGGATACAGCACAGTAAGGAGCTAAACAGAACCTGCTGCCTGAATGGGGGAACCTGCATGCTGGGGTCCT
TTTGTGCCTGCCCTCCCTCCTTCTACGGACGGAACTGTGAGCACGATGTGCGCAAAGAGAACTGTGGGTC
TGTGCCCCATGACACCTGGCTGCCCAAGAAGTGTTCCCTGTGTAAATGCTGGCACGGTCAGCTCCGCTGC
TTTCCTCAGGCATTTCTACCCGGCTGTGATGGCCTTGTGATGGATGAGCACCTCGTGGCTTCCAGGACTC
CAGAACTACCACCGTCTGCACGTACTACCACTTTTATGCTAGTTGGCATCTGCCTTTCTATACAAAGCTA
CTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209841 protein sequence
Red=Cloning site Green=Tags(s)

MDCRKMARFSYSVIWIMAISKAFELGLVAGLGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPM
GIQHSKELNRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRC
FPQAFLPGCDGLVMDEHLVASRTPELPPSARTTTFMLVGICLSIQSYY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003212
ORF Size 564 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003212.4
RefSeq Size 2174 bp
RefSeq ORF 567 bp
Locus ID 6997
UniProt ID P13385
Cytogenetics 3p21.31
Protein Families Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
MW 21.1 kDa
Summary This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
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"NM_003212" in other vectors (6)
SKU Description Size Price
RC209841L1 Lenti ORF clone of Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209841L2 Lenti ORF clone of Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC209841L3 Lenti ORF clone of Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209841L4 Lenti ORF clone of Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG209841 TDGF1 (tGFP-tagged) - Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1 10 ug
$489.00 $500.00
SC303284 TDGF1 (untagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1 10 ug
$300.00

Plasmid Map for RC209841

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209841
Circular map for RC209841

file-alt Citations

Investigating the role of CRIPTO-1 (TDGF-1) in glioblastoma multiforme U87 cell line
Alowaidi, Hashimi, Nguyen et al
J Cell Biochem (2019) 120 (5), 7412-7427 DOI: 10.1002/jcb.28015
Cited by 11
AI Summary: OriGene products contributed to this study by providing the Cripto-1 (TDGF-1) overexpression plasmid (Cat# RC215307) used to upregulate Cripto-1 in U87 GBM cells, enabling the investigation of its role in glioblastoma pathogenesis and progression.
Showing 1-1 of 1 papers.
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