IL7R alpha (IL7R) (NM_002185) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IL7R alpha |
| Synonyms | CD127; CDW127; IL-7R-alpha; IL7RA; ILRA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002185 |
| ORF Size | 1377 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002185.5 |
| RefSeq Size | 4617 bp |
| RefSeq ORF | 1380 bp |
| Locus ID | 3575 |
| UniProt ID | P16871 |
| Cytogenetics | 5p13.2 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Primary immunodeficiency |
| MW | 51.6 kDa |
| Summary | The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. provided by RefSeq, Dec 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002185" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209687L1 | Lenti ORF clone of Human interleukin 7 receptor (IL7R), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209687L2 | Lenti ORF clone of Human interleukin 7 receptor (IL7R), mGFP tagged | 10 ug |
$757.00
|
|
| RC209687L3 | Lenti ORF clone of Human interleukin 7 receptor (IL7R), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209687L4 | Lenti ORF clone of Human interleukin 7 receptor (IL7R), mGFP tagged | 10 ug |
$757.00
|
|
| RG209687 | IL7R (tGFP-tagged) - Human interleukin 7 receptor (IL7R) | 10 ug |
$489.00
$657.00
|
|
| SC303141 | IL7R (untagged)-Human interleukin 7 receptor (IL7R) | 10 ug |
$457.00
|
|
Citations
Roles of IL-7R Induced by Interactions between Cancer Cells and Macrophages in the Progression of Esophageal Squamous Cell Carcinoma
Kitamura, Koma, Tanigawa et al
Cancers (Basel) (2023) 15 (2) DOI: 10.3390/cancers15020394
Kitamura, Koma, Tanigawa et al
Cancers (Basel) (2023) 15 (2) DOI: 10.3390/cancers15020394
Cited by 16
AI Summary: OriGene products pCMV6-IL7R (#RC209687) and pCMV6-Entry (#PS100001) were used to overexpress IL-7R and as a negative control, respectively, in ESCC cell lines to study the role of IL-7R in cancer progression.
Snippet: … Overexpression of IL-7R in ESCC Cell Lines The expression plasmid pCMV6-IL7R (#RC209687, OriGene Technologies, Rockville, MD, USA), which encodes the IL7R ORF, was employed to overexpress IL-7R in the ESCC cell lines. Lipofectamine 3000 reagent (Thermo Fisher Scientific) was used to tra;- ESCC cells transfected with empty pCMV6-Entry vectors (#PS100001, OriGene Technologies) were used as negative controls (control). …
Mutational Analysis of CD127 and Its Role in Immunological Diseases
Cavar
thesis (2016)
Cavar
thesis (2016)
Snippet: To develop cell lines that expressed WT or mutant CD127 protein, I originally tried to transfect an eukaryotic expression plasmid, pCMV6-CD127 (Plasmid Clone # RC209687;OriGene USA;Rockville, MD) in Jurkat cells and use G418 sulfate (Wisent BioProducts;St-Bruno, QC) to select for positive clones
Showing 1-2 of 2 papers.
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