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LACTB (NM_032857) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC209605
LACTB (Myc-DDK-tagged)-Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $509.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "LACTB or NM_032857"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LACTB
Synonyms G24; MRPL56
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209605 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCGGCTCATGTCAGCAGTGACTGCCCGGGCTGCCGCCCCCGGGGGCTTGGCCTCAAGCTGCGGAC
GACGCGGGGTCCATCAGCGCGCCGGGCTGCCGCCTCTCGGCCACGGCTGGGTCGGGGGCCTCGGGCTGGG
GCTGGGGCTGGCGCTCGGGGTGAAGCTGGCAGGTGGGCTGAGGGGCGCGGCCCCGGCGCAGTCCCCCGCG
GCCCCCGACCCTGAGGCGTCGCCTCTGGCCGAGCCGCCACAGGAGCAGTCCCTCGCCCCGTGGTCTCCGC
AGACCCCGGCGCCGCCCTGCTCCAGGTGCTTCGCCAGAGCCATCGAGAGCAGCCGCGACCTGCTGCACAG
GATCAAGGATGAGGTGGGCGCACCGGGCATAGTGGTTGGAGTTTCTGTAGATGGAAAAGAAGTCTGGTCA
GAAGGTTTAGGTTATGCTGATGTTGAGAACCGTGTACCATGTAAACCAGAGACAGTTATGCGAATTGCTA
GCATCAGCAAAAGTCTCACCATGGTTGCTCTTGCCAAATTGTGGGAAGCAGGGAAACTGGATCTTGATAT
TCCAGTACAACATTATGTTCCCGAATTCCCAGAAAAAGAATATGAAGGTGAAAAGGTTTCTGTCACAACA
AGATTACTGATTTCCCATTTAAGTGGAATTCGTCATTATGAAAAGGACATAAAAAAGGTGAAAGAAGAGA
AAGCTTATAAAGCCTTGAAGATGATGAAAGAGAATGTTGCATTTGAGCAAGAAAAAGAAGGCAAAAGTAA
TGAAAAGAATGATTTTACTAAATTTAAAACAGAGCAGGAGAATGAAGCCAAATGCCGGAATTCAAAACCT
GGCAAGAAAAAGAATGATTTTGAACAAGGCGAATTATATTTGAGAGAAAAGTTTGAAAATTCAATTGAAT
CCCTAAGATTATTTAAAAATGATCCTTTGTTCTTCAAACCTGGTAGTCAGTTTTTGTATTCAACTTTTGG
CTATACCCTACTGGCAGCCATAGTAGAGAGAGCTTCAGGATGTAAATATTTGGACTATATGCAGAAAATA
TTCCATGACTTGGATATGCTGACGACTGTGCAGGAAGAAAACGAGCCAGTGATTTACAATAGAGCAAGAT
TTTATGTTTACAATAAAAAGAAACGTCTTGTCAACACACCTTACGTGGATAACTCCTATAAATGGGCTGG
TGGTGGATTTCTGTCTACAGTGGGTGACCTTCTGAAATTTGGGAATGCAATGCTTTATGGTTACCAAGTT
GGGCTGTTTAAGAACTCAAATGAAAATCTTTTACCTGGATACCTCAAACCAGAAACAATGGTTATGATGT
GGACCCCAGTCCCTAACACAGAGATGTCTTGGGATAAAGAGGGTAAATATGCAATGGCGTGGGGTGTTGT
GGAAAGGAAACAAACGTATGGTTCGTGTAGAAAGCAACGGCATTATGCTTCACATACTGGAGGGGCAGTG
GGTGCCAGTAGTGTCCTGCTGGTCCTTCCTGAAGAACTGGATACAGAGACTATAAATAACAAGGTTCCCC
CAAGAGGAATCATTGTTTCTATCATATGTAACATGCAATCTGTTGGCCTCAATAGCACCGCTTTGAAGAT
TGCCCTTGAATTTGATAAAGACAGATCAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209605 protein sequence
Red=Cloning site Green=Tags(s)

MYRLMSAVTARAAAPGGLASSCGRRGVHQRAGLPPLGHGWVGGLGLGLGLALGVKLAGGLRGAAPAQSPA
APDPEASPLAEPPQEQSLAPWSPQTPAPPCSRCFARAIESSRDLLHRIKDEVGAPGIVVGVSVDGKEVWS
EGLGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDIPVQHYVPEFPEKEYEGEKVSVTT
RLLISHLSGIRHYEKDIKKVKEEKAYKALKMMKENVAFEQEKEGKSNEKNDFTKFKTEQENEAKCRNSKP
GKKKNDFEQGELYLREKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGCKYLDYMQKI
FHDLDMLTTVQEENEPVIYNRARFYVYNKKKRLVNTPYVDNSYKWAGGGFLSTVGDLLKFGNAMLYGYQV
GLFKNSNENLLPGYLKPETMVMMWTPVPNTEMSWDKEGKYAMAWGVVERKQTYGSCRKQRHYASHTGGAV
GASSVLLVLPEELDTETINNKVPPRGIIVSIICNMQSVGLNSTALKIALEFDKDRSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032857
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032857.5
RefSeq Size 1976 bp
RefSeq ORF 1644 bp
Locus ID 114294
UniProt ID P83111
Cytogenetics 15q22.2
Domains beta-lactamase
Protein Families Protease
MW 60.7 kDa
Summary This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. provided by RefSeq, Dec 2013
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Documents
Resources
"NM_032857" in other vectors (4)
SKU Description Size Price
RC209605L3 Lenti ORF clone of Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$809.00
RC209605L4 Lenti ORF clone of Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$809.00
RG209605 LACTB (tGFP-tagged) - Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$709.00
SC111862 LACTB (untagged)-Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$510.00

Plasmid Map for RC209605

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209605
Circular map for RC209605

file-alt Citations

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