IMP3 (IGF2BP3) (NM_006547) Human Tagged ORF Clone
SKU
RC209597
IGF2BP3 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3)
$808.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IMP3 |
| Synonyms | CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006547 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006547.3 |
| RefSeq Size | 4168 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 10643 |
| UniProt ID | O00425 |
| Cytogenetics | 7p15.3 |
| Domains | KH, RRM |
| MW | 63.7 kDa |
| Summary | The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006547" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209597L1 | Lenti ORF clone of Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), Myc-DDK-tagged | 10 ug |
$1,108.00
|
|
| RC209597L2 | Lenti ORF clone of Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), mGFP tagged | 10 ug |
$1,108.00
|
|
| RC209597L3 | Lenti ORF clone of Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), Myc-DDK-tagged | 10 ug |
$1,108.00
|
|
| RC209597L4 | Lenti ORF clone of Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), mGFP tagged | 10 ug |
$1,108.00
|
|
| RG209597 | IGF2BP3 (tGFP-tagged) - Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) | 10 ug |
$489.00
$1,008.00
|
|
| SC111161 | IGF2BP3 (untagged)-Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) | 10 ug |
$810.00
|
|
| SC324059 | IGF2BP3 (untagged)-Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) | 10 ug |
$810.00
|
|
Citations
THADA fusion is a mechanism of IGF2BP3 activation and IGF1R signaling in thyroid cancer
Panebianco, Kelly, Liu et al
PNAS (2017) 114 (9), 2307-2312 DOI: 10.1073/pnas.1614265114
Panebianco, Kelly, Liu et al
PNAS (2017) 114 (9), 2307-2312 DOI: 10.1073/pnas.1614265114
AI Summary: OriGene’s IGF2BP3 expression vector (RC209597) was used in this study to investigate the role of IGF2BP3 overexpression in thyroid cancer driven by THADA fusion.
Snippet: ...anti-IGF2BP3 rabbit mouse monoclonal antibody (clone 69.1, Dako;1...expression vector (RC209597) (OriGene Technologies) using X...with the appropriate primary antibodies. Membranes were subsequently...HRP-conjugated secondary antibody and signals were detected...
Overexpression of the RNA-binding proteins Lin28B and IGF2BP3 (IMP3) is associated with chemoresistance and poor disease outcome in ovarian cancer
Hsu, Shen, Huang et al
Br. J. Cancer (2015) 113 (3), 414-24 DOI: 10.1038/bjc.2015.254
Hsu, Shen, Huang et al
Br. J. Cancer (2015) 113 (3), 414-24 DOI: 10.1038/bjc.2015.254
Cited by 130
AI Summary: OriGene’s IMP3 overexpression plasmid (Cat. No. RC209597) was used to transfect SKOV3 ovarian cancer cells, enabling the study of how increased IMP3 levels affect chemoresistance and disease outcomes in ovarian cancer.
Snippet: No. RC209597, OriGene Technologies, Rockville, MD, USA) or the corresponding empty vector were transfected into SKOV3 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's protocol. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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