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COQ3 (NM_017421) Human Tagged ORF Clone

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SKU
RC209576
COQ3 (Myc-DDK-tagged)-Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COQ3
Synonyms bA9819.1; DHHBMT; DHHBMTASE; UG0215E05
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGTGGCCGTAAGCTGGGCTCCTCCGGGGGTTGGTTTTTAAGAGTGCTGGGGCCTGGAGGCTGTA
ATACAAAAGCTGCGCGTCCCTTAATTTCCTCGGCGGTTTATGTGAAGAACCAGCTCAGTGGGACTCTACA
GATTAAACCAGGGGTTTTCAATGAATACAGAACCATATGGTTCAAATCCTACAGGACGATCTTTTCCTGT
TTGAACAGAATAAAGAGTTTCAGGTACCCTTGGGCGAGACTGTACAGTACTTCCCAAACCACTGTCGACA
GCGGTGAGGTAAAAACCTTCTTGGCCCTGGCTCACAAATGGTGGGATGAACAAGGAGTATATGCACCTCT
TCATTCCATGAATGACCTGAGGGTGCCATTTATTAGGGACAATCTTCTGAAAACAATTCCTAATCACCAG
CCAGGAAAACCTTTGTTGGGGATGAAGATTCTTGACGTTGGCTGTGGTGGTGGGCTGTTAACTGAACCTC
TAGGGCGGCTTGGGGCTTCAGTTATTGGAATCGACCCTGTGGATGAGAACATTAAAACAGCACAATGCCA
TAAATCATTTGATCCAGTCCTGGATAAGAGAATAGAGTACAGAGTGTGTTCCCTGGAAGAGATTGTGGAA
GAGACTGCAGAAACATTTGATGCTGTTGTAGCTTCTGAAGTTGTAGAACATGTGATTGATCTAGAAACAT
TTTTACAGTGCTGCTGTCAAGTGTTAAAACCCGGTGGTTCTTTATTCATTACTACAATCAACAAAACACA
ACTTTCCTATGCCTTGGGAATTGTTTTTTCAGAGCAAATTGCAGGTATTGTACCAAAAGGTACTCATACA
TGGGAGAAGTTTGTTTCACCTGAAACACTAGAGAGCATTCTGGAATCAAATGGTCTGTCAGTTCAAACAG
TGGTAGGAATGCTCTATAACCCCTTCTCAGGTTACTGGCATTGGAGTGAAAATACCAGCCTTAACTATGC
AGCTCATGCTGTGAAATCCAGGGTCCAGGAACACCCAGCCTCTGCTGAGTTTGTTTTAAAGGGAGAAACA
GAAGAGCTCCAAGCTAATGCCTGCACCAATCCAGCTGTGCATGAAAAGCTGAAGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209576 protein sequence
Red=Cloning site Green=Tags(s)

MWSGRKLGSSGGWFLRVLGPGGCNTKAARPLISSAVYVKNQLSGTLQIKPGVFNEYRTIWFKSYRTIFSC
LNRIKSFRYPWARLYSTSQTTVDSGEVKTFLALAHKWWDEQGVYAPLHSMNDLRVPFIRDNLLKTIPNHQ
PGKPLLGMKILDVGCGGGLLTEPLGRLGASVIGIDPVDENIKTAQCHKSFDPVLDKRIEYRVCSLEEIVE
ETAETFDAVVASEVVEHVIDLETFLQCCCQVLKPGGSLFITTINKTQLSYALGIVFSEQIAGIVPKGTHT
WEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGET
EELQANACTNPAVHEKLKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017421
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017421.4
RefSeq Size 1265 bp
RefSeq ORF 1110 bp
Locus ID 51805
UniProt ID Q9NZJ6
Cytogenetics 6q16.2
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis
MW 41 kDa
Summary Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 PubMed 10777520). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.supplied by OMIM, Mar 2008
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"NM_017421" in other vectors (5)
SKU Description Size Price
RC209576L3 Lenti ORF clone of Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3), Myc-DDK-tagged 10 ug
$757.00
RC209576L4 Lenti ORF clone of Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3), mGFP tagged 10 ug
$757.00
RG209576 COQ3 (tGFP-tagged) - Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3) 10 ug
$489.00 $657.00
SC114104 COQ3 (untagged)-Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3) 10 ug
$457.00
SC324338 COQ3 (untagged)-Human coenzyme Q3 homolog, methyltransferase (S. cerevisiae) (COQ3) 10 ug
$457.00

Plasmid Map for RC209576

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209576
Circular map for RC209576

file-alt Citations

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