DNase II (DNASE2) (NM_001375) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DNase II |
| Synonyms | DNASE2A; DNL; DNL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001375 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001375.3 |
| RefSeq Size | 2011 bp |
| RefSeq ORF | 1083 bp |
| Locus ID | 1777 |
| UniProt ID | O00115, A0A024R7F4 |
| Cytogenetics | 19p13.13 |
| Domains | DNase_II |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome |
| MW | 39.6 kDa |
| Summary | This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001375" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209573L1 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209573L2 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), mGFP tagged | 10 ug |
$757.00
|
|
| RC209573L3 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209573L4 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), mGFP tagged | 10 ug |
$757.00
|
|
| RG209573 | DNASE2 (tGFP-tagged) - Human deoxyribonuclease II, lysosomal (DNASE2) | 10 ug |
$489.00
$657.00
|
|
| SC119284 | DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2) | 10 ug |
$457.00
|
|
| SC322699 | DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2) | 10 ug |
$457.00
|
|
Citations
Mitophagy bridges DNA sensing with metabolic adaption to expand lung cancer stem-like cells
Liu, Shan, Yuan et al
EMBO Rep (2022)
Liu, Shan, Yuan et al
EMBO Rep (2022)
Cited by 34
AI Summary: In this study, OriGene's DNase II expression plasmid (RC209573) was used to investigate the role of DNase II in the mitophagy-mediated expansion of lung cancer stem-like cells.
Type I interferon-mediated autoinflammation due to DNase II deficiency
Rodero, Tesser, Bartok et al
Nat Commun (2017) 8 (1), 2176 DOI: 10.1038/s41467-017-01932-3
Rodero, Tesser, Bartok et al
Nat Commun (2017) 8 (1), 2176 DOI: 10.1038/s41467-017-01932-3
AI Summary: OriGene’s Human DNase II gene ORF cDNA clone expression plasmid (pCMV6-RC209573) was used in this study to investigate the functional consequences of DNASE2 mutations in patients with type I interferon-mediated autoinflammation.
Snippet: Article.;Human DNase II gene ORF cDNA clone expression plasmid with a C-terminal Myc-FLAG tag, pCMV6-RC209573, was purchased from OriGene (OriGene Technologies, Rockville, Maryland, USA)
Toll-like Receptor 9 Can be Activated by Endogenous Mitochondrial DNA to Induce Podocyte Apoptosis
Bao, Xia, Liang et al
Sci Rep (2016) 6, 22579 DOI: 10.1038/srep22579
Bao, Xia, Liang et al
Sci Rep (2016) 6, 22579 DOI: 10.1038/srep22579
Cited by 111
AI Summary: In this study, the DNASE human cDNA from OriGene was used to overexpress DNase 2 in cultured podocytes, enabling the researchers to investigate the role of DNase 2 in modulating TLR9-mediated podocyte apoptosis.
Snippet: To overexpression TLR9 or DNase 2 in cultured podocytes, TLR9 or DNase 2 expression plasmid, pUNO1-hTLR9 (Invivogen), or DNASE human cDNA (origene), was transfected into cultured podocytes using LipofecTAMINE 2000 (Invitrogen) following the method ... Cite SaveSaving
Showing 1-3 of 3 papers.
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