Sec8 (EXOC4) (NM_021807) Human Tagged ORF Clone
$892.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Sec8 |
| Synonyms | SEC8; SEC8L1; Sec8p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_021807 |
| ORF Size | 2922 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021807.4 |
| RefSeq Size | 4203 bp |
| RefSeq ORF | 2925 bp |
| Locus ID | 60412 |
| UniProt ID | Q96A65 |
| Cytogenetics | 7q33 |
| Domains | Sec8_exocyst |
| Protein Pathways | Tight junction |
| MW | 110.5 kDa |
| Summary | The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021807" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209487L3 | Lenti ORF clone of Human exocyst complex component 4 (EXOC4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,192.00
|
|
| RC209487L4 | Lenti ORF clone of Human exocyst complex component 4 (EXOC4), transcript variant 1, mGFP tagged | 10 ug |
$1,192.00
|
|
| RG209487 | EXOC4 (tGFP-tagged) - Human exocyst complex component 4 (EXOC4), transcript variant 1 | 10 ug |
$789.00
$1,092.00
|
|
| SC102359 | EXOC4 (untagged)-Human exocyst complex component 4 (EXOC4), transcript variant 1 | 10 ug |
$1,198.00
|
|
| SC323875 | EXOC4 (untagged)-Human exocyst complex component 4 (EXOC4), transcript variant 1 | 10 ug |
$893.00
|
|
Citations
SmartBac, a new baculovirus system for large protein complex production
Zhai, Zhang, Yu et al
Journal of Structural Biology: X (2019)
Zhai, Zhang, Yu et al
Journal of Structural Biology: X (2019)
Snippet: All human exocyst genes were purchased from Origene, USA. For each gene, two cycles of PCR were done to produce the target overlapped ends (M0492, NEB). 4V2G was digested by NdeI, KpnI and EcoRI (NEB)
Showing 1-1 of 1 papers.
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in shipping.
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