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KLHL13 (NM_033495) Human Tagged ORF Clone

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SKU
RC209484
KLHL13 (Myc-DDK-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $610.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHL13
Synonyms BKLHD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209484 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATTGAAATGGAAAACGAGCTCTCCTGCTATCTGGAAATTCCCAGTTCCTGTGCTTAAAACATCCA
GGTCAACTCCACTTTCTCCAGCATACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCT
TGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACCTACACGCATCTTTACC
AGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATG
TGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGCTAGTGA
TTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAATGTGCATTAAACTTCATGGTGTG
AGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACC
TTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACTTCTGTAAAGTGTTTCT
CATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGCCAACACCTACAATCTAACCGAA
GTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGTTCTTGA
AACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTAAGCACTGTACTGAACTTGAGCT
CTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAG
AACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACGGTGGATTTCATGAGAA
CTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAATGATGCCATATATGCAGCCAGT
TATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTGCTGAGG
CAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCCCATGAGTGGAAATCGTTAGCCC
CCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACA
GAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGATCCTCGATACAATAAA
TGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGT
ATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAACAAATGA
ATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGGAACTGTGTATGGAGGAGTGATG
TATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACA
AATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTACAGTGGGAGAAAGGCT
CTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTCCTAAGCTGTGAATACTAT
TCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGGTCGCTG
TCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATCGTTGTATGGTAGAGATAGTGCA
GAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGT
GCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGAGAGTCCCCTCTTTCTG
CACCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209484 protein sequence
Red=Cloning site Green=Tags(s)

MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYISLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFT
SNTHSSVVLQGFDQLRLEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGV
SKVGLRKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTE
VDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMK
NIRFPLMTPQELINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLR
QQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNK
WMQVASLNEKRTFFHLSALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVM
YISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYY
SPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIR
ACTLTVFPPEETTPSPSRESPLSAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033495
ORF Size 1965 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033495.3
RefSeq Size 3991 bp
RefSeq ORF 1968 bp
Locus ID 90293
UniProt ID Q9P2N7, Q96HC9
Cytogenetics Xq24
Domains BTB, Kelch
Protein Pathways Ubiquitin mediated proteolysis
MW 73.9 kDa
Summary This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
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"NM_033495" in other vectors (6)
SKU Description Size Price
RC209484L1 Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, Myc-DDK-tagged 10 ug
$910.00
RC209484L2 Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, mGFP tagged 10 ug
$910.00
RC209484L3 Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, Myc-DDK-tagged 10 ug
$910.00
RC209484L4 Lenti ORF clone of Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1, mGFP tagged 10 ug
$910.00
RG209484 KLHL13 (tGFP-tagged) - Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1 10 ug
$489.00 $810.00
SC111483 KLHL13 (untagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1 10 ug
$611.00

Plasmid Map for RC209484

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209484
Circular map for RC209484

file-alt Citations

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