KLC3 (NM_177417) Human Tagged ORF Clone

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SKU
RC209427
KLC3 (Myc-DDK-tagged)-Human kinesin light chain 3 (KLC3)
  $469.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLC3
Synonyms KLC2; KLC2L; KLCt; KNS2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209427 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGTGCAGGTAGCGGCTCCTGGAAGTGCAGGGCTGGGCCCAGAGCGCCTGAGCCCTGAGGAGCTGG
TGCGGCAGACGCGGCAAGTGGTCCAGGGGCTGGAGGCGCTGCGGGCAGAGCACCATGGCCTGGCTGGGCA
CCTGGCGGAGGCCCTGGCGGGACAGGGCCCGGCAGCCGGCTTGGAGATGCTGGAGGAAAAGCAGCAGGTG
GTGAGCCACTCGCTGGAGGCCATCGAGCTGGGGCTGGGCGAGGCCCAGGTGCTGCTGGCCCTGTCGGCAC
ATGTGGGTGCACTGGAGGCAGAGAAGCAGCGGCTGCGCTCGCAGGCCCGGCGGCTGGCCCAGGAGAACGT
GTGGCTGCGGGAGGAACTGGAGGAGACGCAGCGGCGGCTTCGGGCCAGCGAGGAGTCCGTGGCCCAGCTG
GAGGAGGAGAAGCGCCACCTGGAGTTCCTGGGGCAGCTGCGACAGTACGACCCACCGGCGGAGAGCCAGC
AGTCTGAGTCCCCGCCTCGCCGAGACAGCCTGGCCTCCCTGTTCCCCAGCGAGGAGGAGGAGAGGAAAGG
TCCTGAGGCCGCAGGAGCAGCAGCTGCTCAGCAGGGTGGCTATGAGATCCCTGCCCGCCTTCGGACCCTG
CATAACCTCGTGATCCAGTACGCGGGGCAGGGCCGCTATGAGGTGGCGGTGCCTCTGTGCCGCCAGGCCT
TGGAGGACCTGGAGCGCAGCTCGGGCCACTGCCACCCTGACGTGGCCACCATGCTCAACATCCTGGCGCT
GGTGTACCGGGACCAGAACAAGTACAAAGAAGCCACAGACCTTCTCCATGATGCCCTGCAGATCCGGGAG
CAGACGCTGGGCCCTGAGCACCCCGCGGTGGCCGCCACGCTCAACAACTTGGCTGTCCTCTATGGGAAGC
GTGGGCGTTACCGGGAGGCAGAGCCCCTGTGCCAGCGCGCTTTGGAGATCCGAGAGAAGGTCCTGGGTGC
TGACCACCCAGATGTGGCCAAGCAGCTCAACAACCTGGCCCTGCTGTGCCAGAACCAGGGCAAGTTTGAG
GACGTGGAGCGGCACTATGCCCGGGCCCTGAGCATCTATGAGGCACTGGGCGGGCCCCATGACCCCAACG
TGGCCAAGACCAAGAACAACCTGGCCTCAGCCTACCTGAAACAGAACAAGTATCAACAAGCGGAAGAGCT
GTACAAAGAAATCCTCCACAAGGAGGACCTACCCGCCCCTCTCGGTGCCCCCAACACAGGCACAGCTGGT
GACGCAGAACAGGCCCTTCGCCGCAGCAGCTCACTCTCCAAGATCCGTGAGTCTATCAGGCGAGGAAGTG
AGAAGCTGGTCTCCCGGCTCCGAGGCGAGGCGGCGGCAGGAGCAGCCGGAATGAAGAGAGCCATGTCACT
CAACACACTGAACGTGGATGCTCCAAGGGCTCCTGGGACTCAGTTTCCCAGCTGGCACCTGGACAAGGCC
CCTCGGACCCTCAGCGCCAGCACCCAGGACCTGAGCCCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209427 protein sequence
Red=Cloning site Green=Tags(s)

MSVQVAAPGSAGLGPERLSPEELVRQTRQVVQGLEALRAEHHGLAGHLAEALAGQGPAAGLEMLEEKQQV
VSHSLEAIELGLGEAQVLLALSAHVGALEAEKQRLRSQARRLAQENVWLREELEETQRRLRASEESVAQL
EEEKRHLEFLGQLRQYDPPAESQQSESPPRRDSLASLFPSEEEERKGPEAAGAAAAQQGGYEIPARLRTL
HNLVIQYAGQGRYEVAVPLCRQALEDLERSSGHCHPDVATMLNILALVYRDQNKYKEATDLLHDALQIRE
QTLGPEHPAVAATLNNLAVLYGKRGRYREAEPLCQRALEIREKVLGADHPDVAKQLNNLALLCQNQGKFE
DVERHYARALSIYEALGGPHDPNVAKTKNNLASAYLKQNKYQQAEELYKEILHKEDLPAPLGAPNTGTAG
DAEQALRRSSSLSKIRESIRRGSEKLVSRLRGEAAAGAAGMKRAMSLNTLNVDAPRAPGTQFPSWHLDKA
PRTLSASTQDLSPH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177417
ORF Size 1512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177417.3
RefSeq Size 1793 bp
RefSeq ORF 1515 bp
Locus ID 147700
UniProt ID Q6P597, A0A024R0V3
Cytogenetics 19q13.32
Protein Families Druggable Genome
MW 55.4 kDa
Summary This gene encodes a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two kinesin light chain (KLC) molecules. KLCs are thought to typically be involved in binding cargo and regulating kinesin activity. In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural components of sperm tails and mitochondria. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_177417" in other vectors (4)
SKU Description Size Price
RC209427L3 Lenti ORF clone of Human kinesin light chain 3 (KLC3), Myc-DDK-tagged 10 ug
$769.00
RC209427L4 Lenti ORF clone of Human kinesin light chain 3 (KLC3), mGFP tagged 10 ug
$769.00
RG209427 KLC3 (tGFP-tagged) - Human kinesin light chain 3 (KLC3) 10 ug
$669.00
SC307092 KLC3 (untagged)-Human kinesin light chain 3 (KLC3) 10 ug
$517.00

Plasmid Map for RC209427

Download sequence for

Cloning scheme for RC209427

Circular map for RC209427

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