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FAM98A (NM_015475) Human Tagged ORF Clone

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SKU
RC209306
FAM98A (Myc-DDK-tagged)-Human family with sequence similarity 98, member A (FAM98A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $482.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FAM98A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209306 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTGTGACCTCATGGAGACTGACATCTTGGAGTCGTTGGAAGATCTAGGTTACAAGGGCCCATTGT
TGGAAGATGGAGCGCTCTCTCAGGCAGTCTCTGCTGGAGCCAGTTCCCCCGAGTTTACCAAACTCTGTGC
TTGGCTGGTGTCTGAATTAAGAGTGCTCTGTAAACTAGAGGAAAACGTGCAAGCAACTAACAGTCCGAGT
GAAGCTGAAGAATTCCAGCTTGAGGTGAGTGGGCTACTAGGGGAGATGAACTGCCCGTATCTTTCACTGA
CATCTGGGGATGTGACCAAGCGCCTTCTCATTCAGAAGAACTGCCTCCTCTTGCTCACATACCTCATCTC
AGAACTAGAAGCTGCCAGAATGCTCTGTGTGAATGCTCCTCCAAAAAAAGCTCAAGAAGGAGGCGGTAGT
GAGGTCTTTCAAGAGTTGAAAGGCATATGTATTGCTCTAGGAATGTCCAAACCTCCAGCCAATATAACTA
TGTTCCAATTCTTCAGCGGGATTGAAAAAAAATTAAAGGAAACATTAGCAAAAGTTCCACCTAATCATGT
GGGAAAGCCTTTACTGAAGAAGCCAATGGGACCAGCCCACTGGGAAAAGATAGAAGCAATTAACCAAGCC
ATAGCCAATGAATATGAAGTCCGGAGAAAGCTGCTAATAAAACGTTTGGATGTCACTGTACAATCCTTTG
GCTGGTCTGACAGAGCTAAGAGCCAGACAGAAAAATTAGCCAAGGTTTACCAGCCGAAACGTTCAGTCTT
ATCCCCTAAAACTACTATTTCTGTTGCCCATCTTTTGGCTGCAAGGCAGGACTTGTCAAAGATTTTAAGG
ACAAGCAGCGGCTCTATAAGAGAAAAGACTGCCTGTGCCATCAATAAGGTGTTGATGGGCAGGGTGCCTG
ACAGAGGTGGTAGACCCAATGAAATCGAACCTCCACCCCCAGAGATGCCACCGTGGCAGAAGAGGCAAGA
TGGCCCCCAGCAGCAAACAGGAGGCCGAGGAGGAGGGAGAGGTGGCTATGAACATTCCTCATACGGAGGA
CGAGGAGGTCATGAACAAGGAGGCGGGAGAGGTGGACGTGGTGGCTATGACCATGGTGGCCGAGGGGGAG
GAAGAGGAAATAAGCATCAAGGAGGCTGGACAGATGGAGGGAGTGGTGGAGGAGGTGGCTACCAAGATGG
TGGTTATCGAGATTCAGGTTTCCAGCCAGGTGGCTATCATGGTGGCCACAGCAGTGGTGGCTATCAAGGC
GGAGGTTATGGTGGCTTCCAAACATCTTCTTCATATACAGGAAGTGGATACCAGGGTGGTGGCTACCAGC
AGGACAATGGATACCAAGATGGCGGGCACCATGGTGATCGTGGTGGTGGTCGTGGTGGGCGAGGTGGTCG
TGGAGGCCGAGGTGGTCGTGCAGGCCAGGGAGGAGGCTGGGGAGGAAGAGGGAGCCAGAATTATCACCAA
GGGGGTCAATTTGAACAGCATTTCCAGCATGGAGGTTATCAGTATAATCATTCTGGATTTGGACAGGGAA
GACATTACACTAGT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209306 protein sequence
Red=Cloning site Green=Tags(s)

MECDLMETDILESLEDLGYKGPLLEDGALSQAVSAGASSPEFTKLCAWLVSELRVLCKLEENVQATNSPS
EAEEFQLEVSGLLGEMNCPYLSLTSGDVTKRLLIQKNCLLLLTYLISELEAARMLCVNAPPKKAQEGGGS
EVFQELKGICIALGMSKPPANITMFQFFSGIEKKLKETLAKVPPNHVGKPLLKKPMGPAHWEKIEAINQA
IANEYEVRRKLLIKRLDVTVQSFGWSDRAKSQTEKLAKVYQPKRSVLSPKTTISVAHLLAARQDLSKILR
TSSGSIREKTACAINKVLMGRVPDRGGRPNEIEPPPPEMPPWQKRQDGPQQQTGGRGGGRGGYEHSSYGG
RGGHEQGGGRGGRGGYDHGGRGGGRGNKHQGGWTDGGSGGGGGYQDGGYRDSGFQPGGYHGGHSSGGYQG
GGYGGFQTSSSYTGSGYQGGGYQQDNGYQDGGHHGDRGGGRGGRGGRGGRGGRAGQGGGWGGRGSQNYHQ
GGQFEQHFQHGGYQYNHSGFGQGRHYTS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_015475
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015475.5
RefSeq Size 2745 bp
RefSeq ORF 1557 bp
Locus ID 25940
UniProt ID Q8NCA5, I6L9E8
Cytogenetics 2p22.3
MW 55.2 kDa
Summary Positively stimulates PRMT1-induced protein arginine methylation (PubMed:28040436). Involved in skeletal homeostasis (By similarity). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity). Promotes colorectal cancer cell malignancy (PubMed:28040436).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015475" in other vectors (4)
SKU Description Size Price
RC209306L3 Lenti ORF clone of Human family with sequence similarity 98, member A (FAM98A), Myc-DDK-tagged 10 ug
$782.00
RC209306L4 Lenti ORF clone of Human family with sequence similarity 98, member A (FAM98A), mGFP tagged 10 ug
$782.00
RG209306 FAM98A (tGFP-tagged) - Human family with sequence similarity 98, member A (FAM98A) 10 ug
$489.00 $682.00
SC304320 FAM98A (untagged)-Human family with sequence similarity 98, member A (FAM98A) 10 ug
$531.00

Plasmid Map for RC209306

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209306
Circular map for RC209306

file-alt Citations

FAM98A promotes proliferation of non-small cell lung cancer cells via the P38-ATF2 signaling pathway
Zheng, Liu, Wang et al
Cancer Manag Res (2018) 10, 2269-2278 DOI: 10.2147/CMAR.S163323
AI Summary: OriGene products, specifically the pCMV6-ddk-myc and pCMV6-ddk-myc-FAM98A plasmids, were used for overexpression studies to investigate the role of FAM98A in promoting proliferation of non-small cell lung cancer cells in this research.
Snippet: Details of cell culture were described previously.13. Plasmid transfection and siRNA treatment. The plasmids pCMV6-ddk-myc and pCMV6-ddk-myc-FAM98A were obtained from Origene (RC209306;Origene, Rockville, MD, USA)
FigureFigureFigure
Showing 1-1 of 1 papers.
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