Embigin homolog (EMB) (NM_198449) Human Tagged ORF Clone

  • TrueORF®
SKU
RC209281
EMB (Myc-DDK-tagged)-Human embigin (EMB)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Embigin homolog
Synonyms GP70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209281 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGCCCTCCCCGGCCTGCTGGAGGCCAGGGCGCGTACGCCCCGGCTGCTCCTCCTCCAGTGCCTTC
TCGCTGCCGCGCGCCCAAGCTCGGCGGACGGCAGTGCCCCAGATTCGCCTTTTACAAGTCCACCTCTCAG
AGAAGAAATAATGGCAAATAACTTTTCCTTGGAGAGTCATAACATATCACTGACTGAACATTCTAGTATG
CCAGTAGAAAAAAATATCACTTTAGAAAGGCCTTCTAATGTAAATCTCACATGCCAGTTCACAACATCTG
GGGATTTGAATGCAGTAAATGTGACTTGGAAAAAAGATGGTGAACAACTTGAGAATAATTATCTTGTCAG
TGCAACAGGAAGCACCTTGTATACCCAATACAGGTTCACCATCATTAATAGCAAACAAATGGGAAGTTAT
TCTTGTTTCTTTCGAGAGGAAAAGGAACAAAGGGGAACATTTAATTTCAAAGTCCCTGAACTTCATGGGA
AAAACAAGCCATTGATCTCTTACGTAGGGGATTCTACTGTCTTGACATGTAAATGTCAAAATTGTTTTCC
TTTAAATTGGACCTGGTACAGTAGTAATGGGAGTGTAAAGGTTCCTGTTGGTGTTCAAATGAATAAATAT
GTGATCAATGGAACATATGCTAACGAAACAAAGCTGAAGATAACACAACTTTTGGAGGAAGATGGGGAAT
CTTACTGGTGCCGTGCACTATTCCAATTAGGCGAGAGTGAAGAACACATTGAGCTTGTGGTGCTGAGCTA
TTTGGTGCCCCTCAAACCATTTCTTGTAATAGTGGCTGAGGTGATTCTTTTAGTGGCCACCATTCTGCTT
TGTGAAAAGTACACACAAAAGAAAAAGAAGCACTCAGATGAGGGGAAAGAATTTGAGCAGATTGAACAGC
TGAAATCAGATGATAGCAATGGTATAGAAAATAATGTCCCCAGGCATAGAAAAAATGAGTCTCTGGGCCA
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209281 protein sequence
Red=Cloning site Green=Tags(s)

MRALPGLLEARARTPRLLLLQCLLAAARPSSADGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSM
PVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSY
SCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKY
VINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVPLKPFLVIVAEVILLVATILL
CEKYTQKKKKHSDEGKEFEQIEQLKSDDSNGIENNVPRHRKNESLGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198449
ORF Size 981 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198449.3
RefSeq Size 4309 bp
RefSeq ORF 984 bp
Locus ID 133418
UniProt ID Q6PCB8
Cytogenetics 5q11.1
Protein Families Transmembrane
MW 36.9 kDa
Summary This gene encodes a transmembrane glycoprotein that is a member of the immunoglobulin superfamily. The encoded protein may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. A pseudogene of this gene is found on chromosome 1. provided by RefSeq, Jan 2009
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Documents
Resources
"NM_198449" in other vectors (6)
SKU Description Size Price
RC209281L1 Lenti ORF clone of Human embigin (EMB), Myc-DDK-tagged 10 ug
$600.00
RC209281L2 Lenti ORF clone of Human embigin (EMB), mGFP tagged 10 ug
$600.00
RC209281L3 Lenti ORF clone of Human embigin (EMB), Myc-DDK-tagged 10 ug
$600.00
RC209281L4 Lenti ORF clone of Human embigin (EMB), mGFP tagged 10 ug
$600.00
RG209281 EMB (tGFP-tagged) - Human embigin (EMB) 10 ug
$489.00 $500.00
SC107828 EMB (untagged)-Human embigin (EMB) 10 ug
$300.00

Plasmid Map for RC209281

Download sequence for

Cloning scheme for RC209281

Circular map for RC209281

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