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CAT1 (SLC7A1) (NM_003045) Human Tagged ORF Clone

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SKU
RC209263
SLC7A1 (Myc-DDK-tagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CAT1
Synonyms ATRC1; CAT-1; ERR; HCAT1; REC1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209263 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTGCAAAGTCCTGCTCAACATTGGGCAGCAGATGCTGCGGCGGAAGGTGGTGGACTGTAGCCGGG
AGGAGACGCGGCTGTCTCGCTGCCTGAACACTTTTGATCTGGTGGCCCTCGGGGTGGGCAGCACACTGGG
TGCTGGTGTCTACGTCCTGGCTGGAGCTGTGGCCCGTGAGAATGCAGGCCCTGCCATTGTCATCTCCTTC
CTGATCGCTGCGCTGGCCTCAGTGCTGGCTGGCCTGTGCTATGGCGAGTTTGGTGCTCGGGTCCCCAAGA
CGGGCTCAGCTTACCTCTACAGCTATGTCACCGTTGGAGAGCTCTGGGCCTTCATCACCGGCTGGAACTT
AATCCTCTCCTACATCATCGGTACTTCAAGCGTAGCGAGGGCCTGGAGCGCCACCTTCGACGAGCTGATA
GGCAGACCCATCGGGGAGTTCTCACGGACACACATGACTCTGAACGCCCCCGGCGTGCTGGCTGAAAACC
CCGACATATTCGCAGTGATCATAATTCTCATCTTGACAGGACTTTTAACTCTTGGTGTGAAAGAGTCGGC
CATGGTCAACAAAATATTCACTTGTATTAACGTCCTGGTCCTGGGCTTCATAATGGTGTCAGGATTTGTG
AAAGGATCGGTTAAAAACTGGCAGCTCACGGAGGAGGATTTTGGGAACACATCAGGCCGTCTCTGTTTGA
ACAATGACACAAAAGAAGGGAAGCCCGGTGTTGGTGGATTCATGCCCTTCGGGTTCTCTGGTGTCCTGTC
GGGGGCAGCGACTTGCTTCTATGCCTTCGTGGGCTTTGACTGCATCGCCACCACAGGTGAAGAGGTGAAG
AACCCACAGAAGGCCATCCCCGTGGGGATCGTGGCGTCCCTCTTGATCTGCTTCATCGCCTACTTTGGGG
TGTCGGCTGCCCTCACGCTCATGATGCCCTACTTCTGCCTGGACAATAACAGCCCCCTGCCCGACGCCTT
TAAGCACGTGGGCTGGGAAGGTGCCAAGTACGCAGTGGCCGTGGGCTCCCTCTGCGCTCTTTCCGCCAGT
CTTCTAGGTTCCATGTTTCCCATGCCTCGGGTTATCTATGCCATGGCTGAGGATGGACTGCTATTTAAAT
TCTTAGCCAACGTCAATGATAGGACCAAAACACCAATAATCGCCACATTAGCCTCGGGTGCCGTTGCTGC
TGTGATGGCCTTCCTCTTTGACCTGAAGGACTTGGTGGACCTCATGTCCATTGGCACTCTCCTGGCTTAC
TCGTTGGTGGCTGCCTGTGTGTTGGTCTTACGGTACCAGCCAGAGCAGCCTAACCTGGTATACCAGATGG
CCAGTACTTCCGACGAGTTAGATCCAGCAGACCAAAATGAATTGGCAAGCACCAATGATTCCCAGCTGGG
GTTTTTACCAGAGGCAGAGATGTTCTCTTTGAAAACCATACTCTCACCCAAAAACATGGAGCCTTCCAAA
ATCTCTGGGCTAATTGTGAACATTTCAACCAGCCTTATAGCTGTTCTCATCATCACCTTCTGCATTGTGA
CCGTGCTTGGAAGGGAGGCTCTCACCAAAGGGGCGCTGTGGGCAGTCTTTCTGCTCGCAGGGTCTGCCCT
CCTCTGTGCCGTGGTCACGGGCGTCATCTGGAGGCAGCCCGAGAGCAAGACCAAGCTCTCATTTAAGGTT
CCCTTCCTGCCAGTGCTCCCCATCCTGAGCATCTTCGTGAACGTCTATCTCATGATGCAGCTGGACCAGG
GCACCTGGGTCCGGTTTGCTGTGTGGATGCTGATAGGCTTCATCATCTACTTTGGCTATGGCCTGTGGCA
CAGCGAGGAGGCGTCCCTGGATGCCGACCAAGCAAGGACTCCTGACGGCAACTTGGACCAGTGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209263 protein sequence
Red=Cloning site Green=Tags(s)

MGCKVLLNIGQQMLRRKVVDCSREETRLSRCLNTFDLVALGVGSTLGAGVYVLAGAVARENAGPAIVISF
LIAALASVLAGLCYGEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYIIGTSSVARAWSATFDELI
GRPIGEFSRTHMTLNAPGVLAENPDIFAVIIILILTGLLTLGVKESAMVNKIFTCINVLVLGFIMVSGFV
KGSVKNWQLTEEDFGNTSGRLCLNNDTKEGKPGVGGFMPFGFSGVLSGAATCFYAFVGFDCIATTGEEVK
NPQKAIPVGIVASLLICFIAYFGVSAALTLMMPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSAS
LLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAY
SLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSK
ISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKV
PFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003045
ORF Size 1887 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003045.4
RefSeq Size 7357 bp
RefSeq ORF 1890 bp
Locus ID 6541
UniProt ID P30825
Cytogenetics 13q12.3
Domains aa_permeases
Protein Families Druggable Genome, Transmembrane
MW 67.6 kDa
Summary High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues.UniProtKB/Swiss-Prot Function
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Documents
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"NM_003045" in other vectors (6)
SKU Description Size Price
RC209263L1 Lenti ORF clone of Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1), Myc-DDK-tagged 10 ug
$885.00
RC209263L2 Lenti ORF clone of Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1), mGFP tagged 10 ug
$885.00
RC209263L3 Lenti ORF clone of Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1), Myc-DDK-tagged 10 ug
$885.00
RC209263L4 Lenti ORF clone of Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1), mGFP tagged 10 ug
$885.00
RG209263 SLC7A1 (tGFP-tagged) - Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1) 10 ug
$785.00
SC118229 SLC7A1 (untagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1) 10 ug
$586.00

Plasmid Map for RC209263

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209263
Circular map for RC209263

file-alt Citations

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