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IKB zeta (NFKBIZ) (NM_001005474) Human Tagged ORF Clone

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SKU
RC209215
NFKBIZ (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $575.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IKB zeta
Synonyms IKBZ; INAP; MAIL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209215 representing NM_001005474
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTTGGCAGGCAGCAGAGAGGCCCCTTTCAAGGTGTTCGGGTAAAGAACTCAGTGAAGGAACTCC
TGTTGCACATCCGAAGTCATAAACAGAAGGCTTCTGGCCAAGCTGTGGATGATTTTAAGACACAAGGTGT
GAACATAGAACAGTTCAGAGAATTGAAGAACACAGTATCATACAGTGGGAAAAGGAAAGGGCCCGATTCG
TTGTCTGATGGACCTGCTTGCAAAAGGCCAGCTCTGTTGCATTCCCAATTTTTGACACCACCTCAAACAC
CAACGCCCGGGGAGAGCATGGAAGATGTTCATCTCAATGAACCCAAACAGGAGAGCAGTGCTGATCTGCT
TCAGAACATTATCAACATTAAGAATGAATGCAGCCCCGTTTCCCTGAACACAGTTCAAGTTAGCTGGCTG
AACCCCGTGGTGGTCCCTCAGAGCTCCCCCGCAGAGCAGTGTCAGGACTTCCATGGAGGGCAGGTCTTTT
CTCCACCTCAGAAATGCCAACCATTCCAAGTCAGGGGCTCCCAACAAATGATAGACCAGGCTTCCCTGTA
CCAGTATTCTCCACAGAACCAGCATGTAGAGCAGCAGCCACACTACACCCACAAACCAACTCTGGAATAC
AGTCCTTTTCCCATACCTCCCCAGTCCCCCGCTTATGAACCAAACCTCTTTGATGGTCCAGAATCACAGT
TTTGCCCAAACCAAAGCTTAGTTTCCCTTCTTGGTGATCAAAGGGAATCTGAGAATATTGCTAATCCCAT
GCAGACTTCCTCCAGTGTTCAGCAGCAAAATGATGCTCACTTGCACAGCTTCAGCATGATGCCCAGCAGC
GCCTGTGAGGCCATGGTGGGGCACGAGATGGCCTCTGACTCTTCAAACACTTCACTGCCATTCTCAAACA
TGGGAAATCCAATGAACACCACACAGTTAGGGAAATCACTTTTTCAGTGGCAGGTGGAGCAGGAAGAAAG
CAAATTGGCAAATATTTCCCAAGACCAGTTTCTTTCAAAGGATGCAGATGGTGACACGTTCCTTCATATT
GCTGTTGCCCAAGGGAGAAGGGCACTTTCCTATGTTCTTGCAAGAAAGATGAATGCACTTCACATGCTGG
ATATTAAAGAGCACAATGGACAGAGTGCCTTTCAGGTGGCAGTGGCTGCCAATCAGCATCTCATTGTGCA
GGATCTGGTGAACATCGGGGCACAGGTGAACACCACAGACTGCTGGGGAAGAACACCTCTGCATGTGTGT
GCTGAGAAGGGCCACTCCCAGGTGCTTCAGGCGATTCAGAAGGGAGCAGTGGGAAGTAATCAGTTTGTGG
ATCTTGAGGCAACTAACTATGATGGCCTGACTCCCCTTCACTGTGCAGTCATAGCCCACAATGCTGTGGT
CCATGAACTCCAGAGAAATCAACAGCCTCATTCACCTGAAGTTCAGGAGCTTTTACTGAAGAATAAGAGT
CTGGTTGATACCATTAAGTGCCTAATTCAAATGGGAGCAGCGGTGGAAGCGAAGGATCGCAAAAGTGGCC
GCACAGCCCTGCATTTGGCAGCTGAAGAAGCAAATCTGGAACTCATTCGCCTCTTTTTGGAGCTGCCCAG
TTGCCTGTCTTTTGTGAATGCAAAGGCTTACAATGGCAACACTGCCCTCCATGTTGCTGCCAGCTTGCAG
TATCGGTTGACACAATTAGATGCTGTCCGCCTGTTGATGAGGAAGGGAGCAGACCCAAGTACTCGGAACT
TGGAGAACGAACAGCCAGTGCATTTGGTTCCCGATGGCCCTGTGGGAGAACAGATCCGACGTATCCTGAA
GGGAAAGTCCATTCAGCAGAGAGCTCCACCGTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209215 representing NM_001005474
Red=Cloning site Green=Tags(s)

MGVGRQQRGPFQGVRVKNSVKELLLHIRSHKQKASGQAVDDFKTQGVNIEQFRELKNTVSYSGKRKGPDS
LSDGPACKRPALLHSQFLTPPQTPTPGESMEDVHLNEPKQESSADLLQNIINIKNECSPVSLNTVQVSWL
NPVVVPQSSPAEQCQDFHGGQVFSPPQKCQPFQVRGSQQMIDQASLYQYSPQNQHVEQQPHYTHKPTLEY
SPFPIPPQSPAYEPNLFDGPESQFCPNQSLVSLLGDQRESENIANPMQTSSSVQQQNDAHLHSFSMMPSS
ACEAMVGHEMASDSSNTSLPFSNMGNPMNTTQLGKSLFQWQVEQEESKLANISQDQFLSKDADGDTFLHI
AVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNIGAQVNTTDCWGRTPLHVC
AEKGHSQVLQAIQKGAVGSNQFVDLEATNYDGLTPLHCAVIAHNAVVHELQRNQQPHSPEVQELLLKNKS
LVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQ
YRLTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005474
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005474.3
RefSeq Size 3801 bp
RefSeq ORF 1857 bp
Locus ID 64332
UniProt ID Q9BYH8
Cytogenetics 3q12.3
Protein Families Druggable Genome
MW 68.2 kDa
Summary This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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"NM_001005474" in other vectors (4)
SKU Description Size Price
RC209215L3 Lenti-ORF clone of NFKBIZ (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2 10 ug
$875.00
RC209215L4 Lenti-ORF clone of NFKBIZ (mGFP-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2 10 ug
$875.00
RG209215 NFKBIZ (tGFP-tagged) - Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2 10 ug
$775.00
SC300963 NFKBIZ (untagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2 10 ug
$576.00

Plasmid Map for RC209215

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209215
Circular map for RC209215

file-alt Citations

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