SLC30A7 (NM_133496) Human Tagged ORF Clone

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SKU
RC209198
SLC30A7 (Myc-DDK-tagged)-Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC30A7
Synonyms ZnT-7; ZNT7; ZnTL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209198 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCCCCTGTCCATCAAAGACGATGAATACAAACCACCCAAGTTCAATTTGTTCGGCAAGATCTCGG
GCTGGTTTAGGTCTATACTGTCCGACAAGACTTCCCGGAACCTGTTTTTCTTCCTGTGCCTGAACCTCTC
TTTCGCTTTTGTGGAACTACTCTACGGCATCTGGAGCAACTGCTTAGGCTTGATTTCCGACTCTTTTCAC
ATGTTTTTCGATAGCACTGCCATTTTGGCTGGACTGGCAGCTTCTGTTATTTCAAAATGGAGAGATAATG
ATGCTTTCTCCTATGGGTATGTTAGAGCGGAAGTTCTGGCTGGCTTTGTCAATGGCCTATTTTTGATCTT
CACTGCTTTTTTTATTTTCTCAGAAGGAGTTGAGAGAGCATTAGCCCCTCCAGATGTCCACCATGAGAGA
CTGCTTCTTGTTTCCATTCTTGGGTTTGTGGTAAACCTAATAGGAATATTTGTTTTCAAACATGGAGGTC
ATGGACATTCTCATGGCTCTGGCCACGGACACAGTCATTCCCTCTTTAATGGTGCTCTAGATCAGGCACA
TGGCCATGTCGATCATTGCCATAGCCATGAAGTGAAACATGGTGCTGCACATAGCCATGATCATGCTCAT
GGACATGGACACTTTCATTCTCATGATGGCCCGTCCTTAAAAGAAACAACAGGACCCAGCAGACAGATTT
TACAAGGTGTATTTTTACATATCCTAGCAGATACACTTGGAAGTATTGGTGTAATTGCTTCTGCCATCAT
GATGCAAAATTTTGGTCTGATGATAGCAGATCCTATCTGTTCAATTCTTATAGCCATTCTTATAGTTGTA
AGTGTTATTCCTCTTTTAAGAGAATCTGTTGGAATATTAATGCAGAGAACTCCTCCCCTATTAGAAAATA
GTCTGCCTCAGTGCTATCAGAGGGTACAGCAGTTGCAAGGAGTTTACAGTTTACAGGAACAGCACTTCTG
GACTTTATGTTCTGACGTTTATGTTGGGACCTTGAAATTAATAGTAGCACCTGATGCTGATGCTAGGTGG
ATTTTAAGCCAAACACATAATATTTTTACTCAGGCTGGAGTGAGACAGCTCTACGTACAGATTGATTTTG
CAGCCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209198 protein sequence
Red=Cloning site Green=Tags(s)

MLPLSIKDDEYKPPKFNLFGKISGWFRSILSDKTSRNLFFFLCLNLSFAFVELLYGIWSNCLGLISDSFH
MFFDSTAILAGLAASVISKWRDNDAFSYGYVRAEVLAGFVNGLFLIFTAFFIFSEGVERALAPPDVHHER
LLLVSILGFVVNLIGIFVFKHGGHGHSHGSGHGHSHSLFNGALDQAHGHVDHCHSHEVKHGAAHSHDHAH
GHGHFHSHDGPSLKETTGPSRQILQGVFLHILADTLGSIGVIASAIMMQNFGLMIADPICSILIAILIVV
SVIPLLRESVGILMQRTPPLLENSLPQCYQRVQQLQGVYSLQEQHFWTLCSDVYVGTLKLIVAPDADARW
ILSQTHNIFTQAGVRQLYVQIDFAAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133496
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133496.3, NP_598003.2
RefSeq Size 8232 bp
RefSeq ORF 1131 bp
Locus ID 148867
UniProt ID Q8NEW0
Cytogenetics 1p21.2
Domains Cation_efflux
Protein Families Transmembrane
MW 41.6 kDa
Summary Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc (Seve et al., 2004 PubMed 15154973).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_133496" in other vectors (4)
SKU Description Size Price
RC209198L3 Lenti ORF clone of Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC209198L4 Lenti ORF clone of Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 1, mGFP tagged 10 ug
$757.00
RG209198 SLC30A7 (tGFP-tagged) - Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 1 10 ug
$489.00 $657.00
SC111532 SLC30A7 (untagged)-Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 1 10 ug
$457.00

Plasmid Map for RC209198

Download sequence for

Cloning scheme for RC209198

Circular map for RC209198

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