Chymotrypsin like protease (CTRL) (NM_001907) Human Tagged ORF Clone

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SKU
RC209072
CTRL (Myc-DDK-tagged)-Human chymotrypsin-like (CTRL)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Chymotrypsin like protease
Synonyms CTRL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209072 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCTGCTCAGCCTGACCCTAAGCCTGGTTCTCCTCGGCTCCTCCTGGGGCTGCGGCATTCCTGCCA
TCAAACCGGCACTGAGCTTCAGCCAGAGGATTGTCAACGGGGAGAATGCAGTGTTGGGCTCCTGGCCCTG
GCAGGTGTCCCTGCAGGACAGCAGCGGCTTCCACTTCTGCGGTGGTTCTCTCATCAGCCAGTCCTGGGTG
GTCACTGCTGCCCACTGCAATGTCAGCCCTGGCCGCCATTTTGTTGTCCTGGGCGAGTATGACCGATCAT
CAAACGCAGAGCCCTTGCAGGTTCTGTCCGTCTCTCGGGCCATTACACACCCTAGCTGGAACTCTACCAC
CATGAACAATGACGTGACGCTGCTGAAGCTCGCCTCGCCAGCCCAGTACACAACACGCATCTCGCCAGTT
TGCCTGGCATCCTCAAACGAGGCTCTGACTGAAGGCCTCACGTGTGTCACCACCGGCTGGGGTCGCCTCA
GTGGCGTGGGCAATGTGACACCAGCACATCTGCAGCAGGTGGCTTTGCCCCTGGTCACTGTGAATCAGTG
CCGGCAGTACTGGGGCTCAAGTATCACTGACTCCATGATCTGTGCAGGTGGCGCAGGTGCCTCCTCGTGC
CAGGGTGACTCCGGAGGCCCTCTTGTCTGCCAGAAGGGAAACACATGGGTGCTTATTGGTATTGTCTCCT
GGGGCACCAAAAACTGCAATGTGCGCGCACCTGCTGTGTATACTCGAGTTAGCAAGTTCAGCACCTGGAT
CAACCAGGTCATAGCCTACAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209072 protein sequence
Red=Cloning site Green=Tags(s)

MLLLSLTLSLVLLGSSWGCGIPAIKPALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWV
VTAAHCNVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLKLASPAQYTTRISPV
CLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSC
QGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001907
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001907.3
RefSeq Size 1197 bp
RefSeq ORF 795 bp
Locus ID 1506
UniProt ID P40313, A0A024R6Z5
Cytogenetics 16q22.1
Protein Families Druggable Genome, Protease
MW 28 kDa
Summary This gene encodes a serine-type endopeptidase with chymotrypsin- and elastase-2-like activities. The gene encoding this zymogen is expressed specifically in the pancreas and likely functions as a digestive enzyme. provided by RefSeq, Sep 2016
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Documents
Resources
"NM_001907" in other vectors (4)
SKU Description Size Price
RC209072L3 Lenti ORF clone of Human chymotrypsin-like (CTRL), Myc-DDK-tagged 10 ug
$600.00
RC209072L4 Lenti ORF clone of Human chymotrypsin-like (CTRL), mGFP tagged 10 ug
$600.00
RG209072 CTRL (tGFP-tagged) - Human chymotrypsin-like (CTRL) 10 ug
$489.00 $500.00
SC324228 CTRL (untagged)-Human chymotrypsin-like (CTRL) 10 ug
$300.00

Plasmid Map for RC209072

Download sequence for

Cloning scheme for RC209072

Circular map for RC209072

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