SUOX (NM_000456) Human Tagged ORF Clone

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SKU
RC209066
SUOX (Myc-DDK-tagged)-Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1
$289.00 $507.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SUOX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209066 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGCTGCACAGAGCTGTGGTCCTCAGGCTCCAACAGGCCTGCAGACTCAAGTCAATCCCCTCAA
GGATCTGCATTCAGGCCTGCTCCACAAATGATTCATTTCAGCCCCAGCGCCCCAGCCTCACCTTCTCTGG
TGATAACTCCAGCACCCAGGGATGGAGAGTCATGGGGACCCTATTAGGTCTCGGTGCAGTGTTGGCCTAT
CAGGACCATCGGTGTAGGGCTGCTCAGGAGTCAACACACATATACACTAAGGAGGAAGTGAGTTCCCACA
CCAGCCCTGAGACTGGGATCTGGGTGACTCTGGGCTCTGAGGTCTTTGATGTCACAGAATTTGTGGACCT
ACATCCAGGGGGGCCTTCAAAGCTGATGCTAGCAGCTGGGGGTCCCCTAGAGCCCTTCTGGGCCCTCTAT
GCTGTTCACAACCAGTCCCATGTGCGTGAGTTACTGGCTCAGTACAAGATTGGGGAGCTGAATCCTGAAG
ACAAGGTAGCCCCCACCGTGGAGACCTCTGACCCTTATGCTGATGATCCTGTACGTCACCCAGCCCTGAA
GGTCAACAGCCAGCGGCCCTTTAATGCAGAGCCTCCCCCTGAGCTGCTGACAGAAAACTACATCACACCC
AACCCTATCTTCTTCACCCGGAACCATCTGCCTGTACCTAACCTGGATCCAGACACCTATCGCTTACACG
TAGTAGGAGCACCTGGGGGTCAGTCACTGTCTCTTTCCCTGGATGACTTGCACAACTTTCCCAGGTACGA
GATCACAGTCACTCTGCAGTGTGCCGGCAACCGACGCTCTGAGATGACTCAGGTCAAAGAAGTAAAAGGT
CTGGAGTGGAGAACAGGAGCCATCAGCACTGCACGCTGGGCTGGGGCACGGCTCTGTGATGTGTTAGCCC
AGGCTGGCCACCAACTCTGTGAAACTGAGGCCCACGTCTGCTTTGAGGGACTGGACTCAGACCCTACTGG
GACTGCCTATGGAGCATCCATCCCTCTGGCTCGGGCCATGGACCCTGAAGCTGAGGTCCTGCTGGCATAT
GAGATGAATGGGCAGCCTCTGCCACGTGACCACGGCTTCCCTGTGCGTGTGGTGGTTCCTGGAGTGGTGG
GTGCCCGCCATGTCAAATGGCTGGGCAGAGTGAGTGTGCAGCCAGAGGAAAGTTACAGCCACTGGCAACG
GCGGGATTACAAAGGCTTCTCTCCATCTGTGGACTGGGAGACTGTAGATTTTGACTCTGCTCCATCCATT
CAGGAACTTCCTGTCCAGTCCGCCATCACAGAGCCCCGGGATGGAGAGACTGTAGAATCAGGGGAGGTGA
CCATCAAGGGCTATGCATGGAGTGGTGGTGGCAGGGCTGTGATCCGGGTGGATGTGTCTCTGGATGGGGG
CCTAACCTGGCAGGTGGCTAAGCTGGATGGAGAGGAACAGCGCCCCAGGAAGGCCTGGGCATGGCGTCTG
TGGCAGTTGAAAGCCCCTGTGCCAGCTGGACAAAAGGAACTGAACATTGTTTGTAAGGCTGTGGATGATG
GTTACAATGTGCAGCCAGACACCGTGGCCCCAATCTGGAACCTGCGAGGTGTTCTCAGCAATGCCTGGCA
TCGTGTCCATGTCTATGTCTCCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209066 protein sequence
Red=Cloning site Green=Tags(s)

MLLLHRAVVLRLQQACRLKSIPSRICIQACSTNDSFQPQRPSLTFSGDNSSTQGWRVMGTLLGLGAVLAY
QDHRCRAAQESTHIYTKEEVSSHTSPETGIWVTLGSEVFDVTEFVDLHPGGPSKLMLAAGGPLEPFWALY
AVHNQSHVRELLAQYKIGELNPEDKVAPTVETSDPYADDPVRHPALKVNSQRPFNAEPPPELLTENYITP
NPIFFTRNHLPVPNLDPDTYRLHVVGAPGGQSLSLSLDDLHNFPRYEITVTLQCAGNRRSEMTQVKEVKG
LEWRTGAISTARWAGARLCDVLAQAGHQLCETEAHVCFEGLDSDPTGTAYGASIPLARAMDPEAEVLLAY
EMNGQPLPRDHGFPVRVVVPGVVGARHVKWLGRVSVQPEESYSHWQRRDYKGFSPSVDWETVDFDSAPSI
QELPVQSAITEPRDGETVESGEVTIKGYAWSGGGRAVIRVDVSLDGGLTWQVAKLDGEEQRPRKAWAWRL
WQLKAPVPAGQKELNIVCKAVDDGYNVQPDTVAPIWNLRGVLSNAWHRVHVYVSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000456
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000456.2, NP_000447.2
RefSeq Size 2564 bp
RefSeq ORF 1638 bp
Locus ID 6821
UniProt ID P51687, A0A024RB79
Cytogenetics 12q13.2
Domains heme_1, Mo-co_dimer, oxidored_molyb
Protein Families Druggable Genome
Protein Pathways Sulfur metabolism
MW 60.3 kDa
Summary Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000456" in other vectors (7)
SKU Description Size Price
RC209066L1 Lenti ORF clone of Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$807.00
RC209066L2 Lenti ORF clone of Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$807.00
RC209066L3 Lenti ORF clone of Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$807.00
RC209066L4 Lenti ORF clone of Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$807.00
RG209066 SUOX (tGFP-tagged) - Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $707.00
SC119872 SUOX (untagged)-Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$508.00
SC323864 SUOX (untagged)-Human sulfite oxidase (SUOX), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$508.00

Plasmid Map for RC209066

Download sequence for

Cloning scheme for RC209066

Circular map for RC209066

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