MLT5 (TESMIN) (NM_004923) Human Tagged ORF Clone

  • TrueORF®
SKU
RC209063
MTL5 (Myc-DDK-tagged)-Human metallothionein-like 5, testis-specific (tesmin) (MTL5), transcript variant 1
$289.00 $473.00
2 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MLT5
Synonyms CXCDC2; MTL5; MTLT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209063 representing NM_004923.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGGAGGGCCCTCTGCCGGGCGGGCTGCCCAGCCCCGAGGATGCGATGGTGACGGAGCTCTTAAGC
CCCGAGGGTCCGTTCGCTTCGGAGAACATCGGCCTGAAGGCCCCCGTGAAGTACGAGGAGGACGAGTTC
CACGTCTTCAAAGAAGCGTACCTGGGCCCGGCGGACCCCAAGGAACCCGTCCTGCACGCGTTCAACCCC
GCGCTGGGCGCCGACTGCAAGGGCCAGGTCAAGGCGAAGCTCGCGGGGGGCGACAGCGACGGCGGGGAG
CTCCTCGGGGAGTACCCCGGGATCCCAGAGCTCAGCGCGCTGGAGGACGTCGCGCTCCTGCAGGCCCCG
CAGCCGCCCGCCTGCAACGTGCACTTCCTGTCCTCGCTGCTACCCGCGCACCGCAGCCCGGCGGTGTTG
CCCCTGGGCGCCTGGGTCCTGGAAGGAGCCTCCCACCCGGGCGTCCGCATGATCCCAGTTGAAATCAAG
GAAGCAGGTGGTACTACTACAAGTAATAATCCGGAAGAAGCAACTTTGCAGAATCTTCTTGCTCAGGAA
TCCTGTTGCAAGTTCCCATCGTCCCAGGAACTAGAGGATGCCTCCTGCTGTTCTCTTAAGAAAGATTCC
AACCCAATGGTGATATGCCAATTGAAAGGGGGCACACAAATGCTATGTATAGACAATTCTAGAACAAGA
GAACTAAAAGCACTCCATTTGGTTCCTCAGTATCAAGATCAAAATAATTATCTACAGTCAGATGTCCCT
AAACCAATGACTGCTTTAGTAGGGAGATTTTTGCCAGCATCAACAAAATTAAATCTCATTACACAACAA
CTTGAGGGAGCCTTACCATCGGTAGTCAACGGGTCTGCTTTCCCCTCGGGATCAACTCTTCCAGGACCA
CCAAAAATAACTTTGGCTGGGTACTGTGACTGCTTTGCCAGTGGGGACTTTTGCAACAACTGCAATTGT
AATAATTGTTGCAACAACTTGCATCATGATATTGAACGGTTTAAAGCCATTAAGGCATGTCTTGGTAGA
AATCCAGAAGCTTTCCAGCCAAAAATTGGGAAGGGCCAATTGGGCAATGTCAAGCCCCAGCACAACAAA
GGGTGCAACTGCAGGAGGTCAGGCTGCCTGAAGAATTACTGCGAGTGCTATGAGGCCCAAATTATGTGT
TCTTCTATTTGCAAATGCATTGGTTGCAAAAATTATGAAGAAAGCCCAGAACGAAAGACACTAATGAGC
ATGCCAAACTACATGCAGACTGGAGGTTTGGAAGGCAGCCATTACCTGCCACCAACGAAATTTTCAGGA
CTTCCAAGATTCAGTCACGATAGGCGGCCTTCCTCATGCATCTCCTGGGAGGTGGTGGAGGCCACATGC
GCCTGCCTGCTTGCTCAGGGAGAAGAGGCCGAGAAAGAACACTGCTCCAAGTGCCTGGCAGAGCAGATG
ATCCTGGAGGAATTTGGAAGGTGCTTATCACAGATTCTCCACACTGAGTTTAAATCTAAGGGATTGAAA
ATGGAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>Peptide sequence encoded by RC209063
Blue=ORF Red=Cloning site Green=Tag(s)

MEEGPLPGGLPSPEDAMVTELLSPEGPFASENIGLKAPVKYEEDEFHVFKEAYLGPADPKEPVLHAFNP
ALGADCKGQVKAKLAGGDSDGGELLGEYPGIPELSALEDVALLQAPQPPACNVHFLSSLLPAHRSPAVL
PLGAWVLEGASHPGVRMIPVEIKEAGGTTTSNNPEEATLQNLLAQESCCKFPSSQELEDASCCSLKKDS
NPMVICQLKGGTQMLCIDNSRTRELKALHLVPQYQDQNNYLQSDVPKPMTALVGRFLPASTKLNLITQQ
LEGALPSVVNGSAFPSGSTLPGPPKITLAGYCDCFASGDFCNNCNCNNCCNNLHHDIERFKAIKACLGR
NPEAFQPKIGKGQLGNVKPQHNKGCNCRRSGCLKNYCECYEAQIMCSSICKCIGCKNYEESPERKTLMS
MPNYMQTGGLEGSHYLPPTKFSGLPRFSHDRRPSSCISWEVVEATCACLLAQGEEAEKEHCSKCLAEQM
ILEEFGRCLSQILHTEFKSKGLKME

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_004923
ORF Size 1524 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004923.3, NP_004914.2
RefSeq Size 2535 bp
RefSeq ORF 1527 bp
Locus ID 9633
UniProt ID Q9Y4I5
Cytogenetics 11q13.3
Domains CXC
Protein Families Druggable Genome
MW 55 kDa
Summary Metallothionein proteins are highly conserved low-molecular-weight cysteine-rich proteins that are induced by and bind to heavy metal ions and have no enzymatic activity. They may play a central role in the regulation of cell growth and differentiation and are involved in spermatogenesis. This gene encodes a metallothionein-like protein which has been shown to be expressed differentially in mouse testis and ovary. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_004923" in other vectors (4)
SKU Description Size Price
RC209063L3 Lenti-ORF clone of MTL5 (Myc-DDK-tagged)-Human metallothionein-like 5, testis-specific (tesmin) (MTL5), transcript variant 1 10 ug
$773.00
RC209063L4 Lenti-ORF clone of MTL5 (mGFP-tagged)-Human metallothionein-like 5, testis-specific (tesmin) (MTL5), transcript variant 1 10 ug
$773.00
RG209063 MTL5 (tGFP-tagged) - Human metallothionein-like 5, testis-specific (tesmin) (MTL5), transcript variant 1 10 ug
$673.00
SC117028 MTL5 (untagged)-Human metallothionein-like 5, testis-specific (tesmin) (MTL5), transcript variant 1 10 ug
$474.00

Plasmid Map for RC209063

Download sequence for

Cloning scheme for RC209063

Circular map for RC209063

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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