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KREMEN1 (NM_001039571) Human Tagged ORF Clone

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SKU
RC208991
KREMEN1 (Myc-DDK-tagged)-Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "KREMEN1 or NM_001039571"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KREMEN1
Synonyms FLJ31863; KREMEM1; KREMEN; kringle-coding gene marking the eye and the nose; kringle-containing transmembrane protein 1; kringle containing transmembrane protein 1; KRM1; OTTHUMP00000028977
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208991 representing NM_001039571
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCCAGCCGCCCGCCTCGCCCTGCTCTCCGCCGCGGCGCTCACGCTGGCGGCCCGGCCCGCGC
CTAGCCCCGGCCTCGGCCCCGGACCCGAGTGTTTCACAGCCAATGGTGCGGATTATAGGGGAACACAGAA
CTGGACAGCACTACAAGGCGGGAAGCCATGTCTGTTTTGGAACGAGACTTTCCAGCATCCATACAACACT
CTGAAATACCCCAACGGGGAGGGGGGCCTGGGTGAGCACAACTATTGCAGAAATCCAGATGGAGACGTGA
GCCCCTGGTGCTATGTGGCAGAGCACGAGGATGGTGTCTACTGGAAGTACTGTGAGATACCTGCTTGCCA
GATGCCTGGAAACCTTGGCTGCTACAAGGATCATGGAAACCCACCTCCTCTAACTGGCACCAGTAAAACG
TCCAACAAACTCACCATACAAACTTGCATCAGTTTTTGTCGGAGTCAGAGGTTCAAGTTTGCTGGGATGG
AGTCAGGCTATGCTTGCTTCTGTGGAAACAATCCTGATTACTGGAAGTACGGGGAGGCAGCCAGTACCGA
ATGCAACAGCGTCTGCTTCGGGGATCACACCCAACCCTGTGGTGGCGATGGCAGGATCATCCTCTTTGAT
ACTCTCGTGGGCGCCTGCGGTGGGAACTACTCAGCCATGTCTTCTGTGGTCTATTCCCCTGACTTCCCCG
ACACCTATGCCACGGGGAGGGTCTGCTACTGGACCATCCGGGTTCCGGGGGCCTCCCACATCCACTTCAG
CTTCCCCCTATTTGACATCAGGGACTCGGCGGACATGGTGGAGCTTCTGGATGGCTACACCCACCGTGTC
CTAGCCCGCTTCCACGGGAGGAGCCGCCCACCTCTGTCCTTCAACGTCTCTCTGGACTTCGTCATCTTGT
ATTTCTTCTCTGATCGCATCAATCAGGCCCAGGGATTTGCTGTTTTATACCAAGCCGTCAAGGAAGAACT
GCCACAGGAGAGGCCCGCTGTCAACCAGACGGTGGCCGAGGTGATCACGGAGCAGGCCAACCTCAGTGTC
AGCGCTGCCCGGTCCTCCAAAGTCCTCTATGTCATCACCACCAGCCCCAGCCACCCACCTCAGACTGTCC
CAGGATGGACAGTCTATGGTCTGGCAACTCTCCTCATCCTCACAGTCACAGCCATTGTAGCAAAGATACT
TCTGCACGTCACATTCAAATCCCATCGTGTTCCTGCTTCAGGGGACCTTAGGGATTGTCATCAACCAGGG
ACTTCGGGGGAAATCTGGAGCATTTTTTACAAGCCTTCCACTTCAATTTCCATCTTTAAGAAGAAACTCA
AGGGTCAGAGTCAACAAGATGACCGCAATCCCCTTGTGAGTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208991 representing NM_001039571
Red=Cloning site Green=Tags(s)

MAPPAARLALLSAAALTLAARPAPSPGLGPGPECFTANGADYRGTQNWTALQGGKPCLFWNETFQHPYNT
LKYPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKT
SNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFD
TLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRV
LARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSV
SAARSSKVLYVITTSPSHPPQTVPGWTVYGLATLLILTVTAIVAKILLHVTFKSHRVPASGDLRDCHQPG
TSGEIWSIFYKPSTSISIFKKKLKGQSQQDDRNPLVSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_001039571
ORF Size 1374 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039571.1, NP_001034660.1
RefSeq Size 6115 bp
RefSeq ORF 1376 bp
Locus ID 83999
Cytogenetics 22q12.1
Protein Families Druggable Genome, Transmembrane
MW 50.18 kDa
Summary This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. provided by RefSeq, Jul 2008
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"NM_001039571" in other vectors (6)
SKU Description Size Price
RC208991L1 Lenti ORF clone of Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4, Myc-DDK-tagged 10 ug
$757.00
RC208991L2 Lenti ORF clone of Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4, mGFP tagged 10 ug
$757.00
RC208991L3 Lenti ORF clone of Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4, Myc-DDK-tagged 10 ug
$757.00
RC208991L4 Lenti ORF clone of Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4, mGFP tagged 10 ug
$757.00
RG208991 KREMEN1 (tGFP-tagged) - Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4 10 ug
$657.00
SC322731 KREMEN1 (untagged)-Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4 10 ug
$457.00

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