NDNL2 (NSMCE3) (NM_138704) Human Tagged ORF Clone

  • TrueORF®
SKU
RC208815
NDNL2 (Myc-DDK-tagged)-Human necdin-like 2 (NDNL2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NDNL2
Synonyms HCA4; LICS; MAGEG1; MAGEL3; NDNL2; NSE3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208815 representing NM_138704
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCAAAAACCGAGGAACCGGGGCCGCTCTGGCGGCCAGGCCGAGAGGGACAGAGACTGGAGCCATA
GCGGAAACCCCGGGGCTTCGCGGGCCGGGGAAGACGCCCGGGTTCTCAGAGACGGCTTTGCCGAGGAGGC
CCCGAGCACGTCCCGCGGGCCGGGCGGCTCGCAGGGGTCGCAGGGCCCCTCGCCTCAGGGCGCCCGCCGG
GCCCAGGCCGCCCCCGCCGTGGGGCCCAGGAGCCAGAAGCAGCTGGAGCTGAAAGTGTCCGAGCTGGTGC
AGTTCTTGCTGATTAAAGACCAGAAGAAGATTCCGATCAAGCGGGCCGACATACTGAAGCACGTCATCGG
GGACTACAAGGACATCTTCCCCGACCTCTTCAAACGGGCCGCCGAGCGCCTCCAGTACGTCTTCGGGTAT
AAGCTGGTGGAACTTGAACCCAAGAGCAACACTTACATCCTCATCAACACCCTGGAGCCTGTGGAGGAGG
ATGCCGAGATGAGGGGTGACCAAGGCACGCCCACTACGGGCCTCCTGATGATCGTCTTAGGGCTCATCTT
TATGAAGGGCAACACCATCAAGGAAACTGAAGCCTGGGACTTTCTGCGGCGCTTAGGGGTCTACCCCACC
AAGAAGCATTTAATTTTCGGAGATCCAAAGAAACTCATTACTGAGGACTTTGTGCGACAGCGTTACCTGG
AATACCGGCGGATACCCCACACCGACCCCGTCGACTACGAATTCCAGTGGGGCCCGCGAACCAACCTGGA
AACCAGCAAGATGAAAGTTCTTAAGTTTGTGGCCAAGGTCCATAATCAAGACCCCAAGGACTGGCCAGCG
CAGTACTGTGAGGCTTTGGCAGATGAGGAGAACAGGGCCAGACCTCAGCCTAGTGGCCCAGCTCCATCCT
CT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208815 representing NM_138704
Red=Cloning site Green=Tags(s)

MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARR
AQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGY
KLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPT
KKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPA
QYCEALADEENRARPQPSGPAPSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138704
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138704.4
RefSeq Size 1748 bp
RefSeq ORF 915 bp
Locus ID 56160
UniProt ID Q96MG7
Cytogenetics 15q13.1
Domains MAGE
MW 34.3 kDa
Summary The protein encoded by this gene is part of the SMC5-6 chromatin reorganizing complex and is a member of the MAGE superfamily. This is an intronless gene. provided by RefSeq, May 2011
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Documents
Resources
"NM_138704" in other vectors (4)
SKU Description Size Price
RC208815L3 Lenti-ORF clone of NDNL2 (Myc-DDK-tagged)-Human necdin-like 2 (NDNL2) 10 ug
$600.00
RC208815L4 Lenti-ORF clone of NDNL2 (mGFP-tagged)-Human necdin-like 2 (NDNL2) 10 ug
$600.00
RG208815 NDNL2 (tGFP-tagged) - Human necdin-like 2 (NDNL2) 10 ug
$500.00
SC321934 NDNL2 (untagged)-Human necdin-like 2 (NDNL2) 10 ug
$300.00

Plasmid Map for RC208815

Download sequence for

Cloning scheme for RC208815

Circular map for RC208815

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