PNPT1 (NM_033109) Human Tagged ORF Clone
$1,075.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PNPT1 |
| Synonyms | COXPD13; DFNB70; old-35; OLD35; PNPASE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_033109 |
| ORF Size | 2349 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_033109.5 |
| RefSeq Size | 4579 bp |
| RefSeq ORF | 2352 bp |
| Locus ID | 87178 |
| UniProt ID | Q8TCS8 |
| Cytogenetics | 2p16.1 |
| Domains | RNase_PH_C |
| Protein Pathways | Purine metabolism, Pyrimidine metabolism, RNA degradation |
| MW | 86 kDa |
| Summary | The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. provided by RefSeq, Dec 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_033109" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208800L3 | Lenti-ORF clone of PNPT1 (Myc-DDK-tagged)-Human polyribonucleotide nucleotidyltransferase 1 (PNPT1) | 10 ug |
$1,375.00
|
|
| RC208800L4 | Lenti-ORF clone of PNPT1 (mGFP-tagged)-Human polyribonucleotide nucleotidyltransferase 1 (PNPT1) | 10 ug |
$1,375.00
|
|
| RG208800 | PNPT1 (tGFP-tagged) - Human polyribonucleotide nucleotidyltransferase 1 (PNPT1) | 10 ug |
$1,275.00
|
|
| SC100684 | PNPT1 (untagged)-Human polyribonucleotide nucleotidyltransferase 1 (PNPT1) | 10 ug |
$1,077.00
|
|
Citations
miR-181d coordinates homologous recombination and anti-tumor immune responses in glioblastoma
Singh, Singh, Mohapatra et al
iScience (2026) 29 (3), 115077 DOI: 10.1016/j.isci.2026.115077
Singh, Singh, Mohapatra et al
iScience (2026) 29 (3), 115077 DOI: 10.1016/j.isci.2026.115077
Cited by 3
AI Summary: OriGene’s pCMV6-Entry-RAD51 plasmid (RC208800) was used in this study to investigate the regulation and functional role of RAD51 by miR-181d in glioblastoma cells.
Showing 1-1 of 1 papers.
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in shipping.
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