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GCSF Receptor (CSF3R) (NM_000760) Human Tagged ORF Clone

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SKU
RC208790
CSF3R (Myc-DDK-tagged)-Human colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $765.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GCSF Receptor
Synonyms CD114; GCSFR; SCN7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208790 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGGCTGGGAAACTGCAGCCTGACTTGGGCTGCCCTGATCATCCTGCTGCTCCCCGGAAGTCTGG
AGGAGTGCGGGCACATCAGTGTCTCAGCCCCCATCGTCCACCTGGGGGATCCCATCACAGCCTCCTGCAT
CATCAAGCAGAACTGCAGCCATCTGGACCCGGAGCCACAGATTCTGTGGAGACTGGGAGCAGAGCTTCAG
CCCGGGGGCAGGCAGCAGCGTCTGTCTGATGGGACCCAGGAATCTATCATCACCCTGCCCCACCTCAACC
ACACTCAGGCCTTTCTCTCCTGCTGCCTGAACTGGGGCAACAGCCTGCAGATCCTGGACCAGGTTGAGCT
GCGCGCAGGCTACCCTCCAGCCATACCCCACAACCTCTCCTGCCTCATGAACCTCACAACCAGCAGCCTC
ATCTGCCAGTGGGAGCCAGGACCTGAGACCCACCTACCCACCAGCTTCACTCTGAAGAGTTTCAAGAGCC
GGGGCAACTGTCAGACCCAAGGGGACTCCATCCTGGACTGCGTGCCCAAGGACGGGCAGAGCCACTGCTG
CATCCCACGCAAACACCTGCTGTTGTACCAGAATATGGGCATCTGGGTGCAGGCAGAGAATGCGCTGGGG
ACCAGCATGTCCCCACAACTGTGTCTTGATCCCATGGATGTTGTGAAACTGGAGCCCCCCATGCTGCGGA
CCATGGACCCCAGCCCTGAAGCGGCCCCTCCCCAGGCAGGCTGCCTACAGCTGTGCTGGGAGCCATGGCA
GCCAGGCCTGCACATAAATCAGAAGTGTGAGCTGCGCCACAAGCCGCAGCGTGGAGAAGCCAGCTGGGCA
CTGGTGGGCCCCCTCCCCTTGGAGGCCCTTCAGTATGAGCTCTGCGGGCTCCTCCCAGCCACGGCCTACA
CCCTGCAGATACGCTGCATCCGCTGGCCCCTGCCTGGCCACTGGAGCGACTGGAGCCCCAGCCTGGAGCT
GAGAACTACCGAACGGGCCCCCACTGTCAGACTGGACACATGGTGGCGGCAGAGGCAGCTGGACCCCAGG
ACAGTGCAGCTGTTCTGGAAGCCAGTGCCCCTGGAGGAAGACAGCGGACGGATCCAAGGTTATGTGGTTT
CTTGGAGACCCTCAGGCCAGGCTGGGGCCATCCTGCCCCTCTGCAACACCACAGAGCTCAGCTGCACCTT
CCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCAGCCGGGACCTCTCGTCCCACT
CCGGTGGTCTTCTCAGAAAGCAGAGGCCCAGCTCTGACCAGACTCCATGCCATGGCCCGAGACCCTCACA
GCCTCTGGGTAGGCTGGGAGCCCCCCAATCCATGGCCTCAGGGCTATGTGATTGAGTGGGGCCTGGGCCC
CCCCAGCGCGAGCAATAGCAACAAGACCTGGAGGATGGAACAGAATGGGAGAGCCACGGGGTTTCTGCTG
AAGGAGAACATCAGGCCCTTTCAGCTCTATGAGATCATCGTGACTCCCTTGTACCAGGACACCATGGGAC
CCTCCCAGCATGTCTATGCCTACTCTCAAGAAATGGCTCCCTCCCATGCCCCAGAGCTGCATCTAAAGCA
CATTGGCAAGACCTGGGCACAGCTGGAGTGGGTGCCTGAGCCCCCTGAGCTGGGGAAGAGCCCCCTTACC
CACTACACCATCTTCTGGACCAACGCTCAGAACCAGTCCTTCTCCGCCATCCTGAATGCCTCCTCCCGTG
GCTTTGTCCTCCATGGCCTGGAGCCCGCCAGTCTGTATCACATCCACCTCATGGCTGCCAGCCAGGCTGG
GGCCACCAACAGTACAGTCCTCACCCTGATGACCTTGACCCCAGAGGGGTCGGAGCTACACATCATCCTG
GGCCTGTTCGGCCTCCTGCTGTTGCTCACCTGCCTCTGTGGAACTGCCTGGCTCTGTTGCAGCCCCAACA
GGAAGAATCCCCTCTGGCCAAGTGTCCCAGACCCAGCTCACAGCAGCCTGGGCTCCTGGGTGCCCACAAT
CATGGAGGAGGATGCCTTCCAGCTGCCCGGCCTTGGCACGCCACCCATCACCAAGCTCACAGTGCTGGAG
GAGGATGAAAAGAAGCCGGTGCCCTGGGAGTCCCATAACAGCTCAGAGACCTGTGGCCTCCCCACTCTGG
TCCAGACCTATGTGCTCCAGGGGGACCCAAGAGCAGTTTCCACCCAGCCCCAATCCCAGTCTGGCACCAG
CGATCAGGTCCTTTATGGGCAGCTGCTGGGCAGCCCCACAAGCCCAGGGCCAGGGCACTATCTCCGCTGT
GACTCCACTCAGCCCCTCTTGGCGGGCCTCACCCCCAGCCCCAAGTCCTATGAGAACCTCTGGTTCCAGG
CCAGCCCCTTGGGGACCCTGGTAACCCCAGCCCCAAGCCAGGAGGACGACTGTGTCTTTGGGCCACTGCT
CAACTTCCCCCTCCTGCAGGGGATCCGGGTCCATGGGATGGAGGCGCTGGGGAGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208790 protein sequence
Red=Cloning site Green=Tags(s)

MARLGNCSLTWAALIILLLPGSLEECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQ
PGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSL
ICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALG
TSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWA
LVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPR
TVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPT
PVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLL
KENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLT
HYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTPEGSELHIIL
GLFGLLLLLTCLCGTAWLCCSPNRKNPLWPSVPDPAHSSLGSWVPTIMEEDAFQLPGLGTPPITKLTVLE
EDEKKPVPWESHNSSETCGLPTLVQTYVLQGDPRAVSTQPQSQSGTSDQVLYGQLLGSPTSPGPGHYLRC
DSTQPLLAGLTPSPKSYENLWFQASPLGTLVTPAPSQEDDCVFGPLLNFPLLQGIRVHGMEALGSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000760
ORF Size 2508 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000760.4
RefSeq Size 3419 bp
RefSeq ORF 2511 bp
Locus ID 1441
UniProt ID Q99062
Cytogenetics 1p34.3
Domains FN3
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Pathways in cancer
MW 92.2 kDa
Summary The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. provided by RefSeq, Aug 2010
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"NM_000760" in other vectors (4)
SKU Description Size Price
RC208790L3 Lenti-ORF clone of CSF3R (Myc-DDK-tagged)-Human colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 1 10 ug
$1,065.00
RC208790L4 Lenti-ORF clone of CSF3R (mGFP-tagged)-Human colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 1 10 ug
$1,065.00
RG208790 CSF3R (tGFP-tagged) - Human colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 1 10 ug
$789.00 $965.00
SC111618 CSF3R (untagged)-Human colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 1 10 ug
$1,149.00

Plasmid Map for RC208790

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208790
Circular map for RC208790

file-alt Citations

JAK/STAT blockade reverses the malignant phenotype of Hodgkin and Reed-Sternberg cells
Fernández, Solórzano, Díaz et al
Blood Adv (2022)
Cited by 10
AI Summary: OriGene contributed to this study by providing the PCMV6-hCSF3R-Myc-DDK tagged plasmid (CAT#: RC208790), which was used for transfecting L-428 cells to investigate CSF3R expression as a biomarker in Hodgkin lymphoma.
Snippet: by immunocytochemistry using an anti-Myc monoclonal antibody (CNIO). The plasmid vector (PCMV6-hCSF3R-Myc-DDK tagged) used for L-428 transfections was obtained from Origene (CAT#: RC208790, Origene, Rockville, MD)
ICC
Showing 1-1 of 1 papers.
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