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ZNF224 (NM_013398) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC208733
ZNF224 (Myc-DDK-tagged)-Human zinc finger protein 224 (ZNF224). Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $647.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF224
Synonyms BMZF-2; BMZF2; KOX22; ZNF27; ZNF233; ZNF255
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208733 ORF sequence, codon optimized.
Due to the complexity of NM_013398, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACTTTTAAGGAGGCGATGACATTTAAAGACGTCGCTGTGGTTTTTACGGAGGAGGAGCTGGGTC
TGCTCGATCTCGCCCAGAGGAAATTGTATCGGGATGTGATGTTGGAGAACTTTAGAAATCTGCTGAGTGT
CGGGCATCAGGCATTTCACCGCGACACCTTCCACTTCCTGAGAGAAGAAAAGATATGGATGATGAAAACG
GCCATCCAGCGGGAGGGTAACAGCGGGGACAAAATTCAGACAGAAATGGAAACGGTTAGCGAGGCCGGCA
CACACCAGGAGTGGTCCTTCCAACAAATTTGGGAAAAGATCGCTTCCGATCTTACTCGCTCTCAGGATTT
GATGATTAATAGCTCCCAGTTCAGTAAAGAGGGTGACTTTCCTTGCCAAACTGAGGCCGGCCTCTCAGTC
ATCCACACGCGGCAGAAGAGCAGCCAGGGTAATGGCTATAAGCCCAGCTTTAGCGACGTGAGCCATTTCG
ACTTTCACCAACAGCTGCACTCAGGAGAAAAGAGCCACACCTGCGATGAATGCGGTAAGAATTTTTGCTA
CATCTCAGCCTTGAGGATTCATCAGCGAGTGCACATGGGCGAGAAGTGTTATAAATGCGACGTGTGCGGA
AAGGAGTTCAGTCAAAGTAGCCACCTGCAGACCCATCAACGGGTACACACCGGCGAGAAGCCTTTCAAAT
GCGTGGAATGTGGAAAGGGCTTTAGTCGACGGTCAGCTCTTAATGTCCACCACAAGCTCCATACCGGGGA
GAAGCCCTATAATTGTGAGGAGTGTGGCAAGGCGTTCATCCACGATTCCCAACTGCAAGAGCATCAGCGC
ATCCACACAGGTGAAAAGCCTTTCAAATGCGACATTTGTGGGAAGAGTTTCTGTGGCCGCTCACGGCTTA
ATCGCCACAGCATGGTACATACCGCCGAGAAGCCCTTCCGGTGCGACACCTGCGATAAGTCTTTTCGACA
GCGGAGCGCTCTGAACTCCCACAGGATGATCCACACCGGAGAGAAGCCATACAAGTGTGAAGAGTGTGGC
AAGGGGTTCATTTGCCGGCGGGACCTGTATACTCACCATATGGTGCACACCGGCGAGAAGCCTTATAACT
GTAAAGAGTGCGGTAAGTCCTTCAGGTGGGCCTCCTGCCTGCTGAAGCACCAGAGGGTCCATTCTGGAGA
GAAACCTTTCAAGTGCGAGGAATGCGGCAAAGGATTTTACACAAACAGTCAGTGCTACAGCCACCAGAGA
AGCCACAGTGGAGAGAAACCCTACAAGTGCGTGGAATGCGGCAAGGGTTATAAGCGGAGGCTCGATCTCG
ATTTCCACCAGCGCGTGCACACCGGAGAAAAGCTGTATAATTGTAAAGAATGTGGTAAATCATTTAGCAG
AGCTCCATGCCTGCTTAAACATGAGCGACTTCACTCAGGCGAAAAGCCCTTCCAGTGTGAAGAATGCGGG
AAACGGTTCACCCAGAACTCTCACTTGCATTCACACCAAAGGGTACACACCGGAGAGAAACCTTACAAGT
GTGAGAAATGCGGTAAGGGGTACAACAGCAAATTTAATCTCGATATGCACCAAAAGGTGCATACAGGTGA
GCGACCGTATAATTGCAAGGAGTGCGGGAAATCTTTTGGATGGGCCTCCTGCCTCCTGAAGCACCAGAGG
CTTCACTCCGGCGAGAAGCCCTTCAAGTGCGAGGAGTGCGGAAAAAGATTCACCCAGAACTCTCAGCTGC
ACAGTCACCAGCGAGTCCATACTGGAGAGAAGCCATATAAGTGTGACGAATGCGGAAAGGGATTCTCATG
GTCCTCTACTCGCCTCACTCATCAACGGCGCCATTCTCGGGAGACACCCCTCAAATGTGAGCAGCATGGC
AAGAACATCGTCCAAAATTCCTTCAGTAAGGTGCAGGAAAAGGTGCACAGCGTTGAGAAGCCCTATAAAT
GTGAAGATTGCGGCAAGGGCTACAATAGACGCTTGAACCTCGACATGCATCAGAGAGTTCACATGGGCGA
GAAAACCTGGAAGTGCCGGGAATGTGACATGTGCTTTTCCCAAGCAAGCAGTCTGCGACTGCACCAGAAC
GTCCACGTAGGCGAGAAACCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208733 representing NM_013398
Red=Cloning site Green=Tags(s)

MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKT
AIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSV
IHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCG
KEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQR
IHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECG
KGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQR
SHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECG
KRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQR
LHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHG
KNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQN
VHVGEKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013398
ORF Size 2121 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013398.1, NM_013398.2, NM_013398.3, NP_037530.1, NP_037530.2
RefSeq Size 3993 bp
RefSeq ORF 2124 bp
Locus ID 7767
UniProt ID Q9NZL3
Cytogenetics 19q13.31
Domains KRAB, zf-C2H2
Protein Families Transcription Factors
MW 82.3 kDa
Summary This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. provided by RefSeq, Jul 2016
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"NM_013398" in other vectors (5)
SKU Description Size Price
RC208733L3 Lenti-ORF clone of ZNF224 (Myc-DDK-tagged)-Human zinc finger protein 224 (ZNF224). Note: ORF is codon optimized 10 ug
$947.00
RC208733L4 Lenti-ORF clone of ZNF224 (mGFP-tagged)-Human zinc finger protein 224 (ZNF224). Note: ORF is codon optimized 10 ug
$947.00
RG208733 ZNF224 (tGFP-tagged) - Human zinc finger protein 224 (ZNF224). Note: ORF is codon optimized 10 ug
$789.00 $847.00
SC124453 ZNF224 (untagged)-Human zinc finger protein 224 (ZNF224) 10 ug
$648.00
SC322685 ZNF224 (untagged)-Human zinc finger protein 224 (ZNF224) 10 ug
$648.00

Plasmid Map for RC208733

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208733
Circular map for RC208733

file-alt Citations

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