Synapsin II (SYN2) (NM_133625) Human Tagged ORF Clone
$542.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Synapsin II |
| Synonyms | SYNII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_133625 |
| ORF Size | 1746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_133625.3, NP_598328.1 |
| RefSeq Size | 3320 bp |
| RefSeq ORF | 1749 bp |
| Locus ID | 6854 |
| UniProt ID | Q92777, Q86VA8, B3KRB3, A0A087X2E3 |
| Cytogenetics | 3p25.2 |
| Domains | Synapsin |
| MW | 63 kDa |
| Summary | This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Polymorphisms in this gene are associated with abnormal presynaptic function and related neuronal disorders, including autism, epilepsy, bipolar disorder and schizophrenia. Alternative splicing of this gene results in multiple transcript variants. The tissue inhibitor of metalloproteinase 4 gene is located within an intron of this gene and is transcribed in the opposite direction. provided by RefSeq, Feb 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_133625" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208555L3 | Lenti-ORF clone of SYN2 (Myc-DDK-tagged)-Human synapsin II (SYN2), transcript variant IIa | 10 ug |
$842.00
|
|
| RC208555L4 | Lenti-ORF clone of SYN2 (mGFP-tagged)-Human synapsin II (SYN2), transcript variant IIa | 10 ug |
$842.00
|
|
| RG208555 | SYN2 (tGFP-tagged) - Human synapsin II (SYN2), transcript variant IIa | 10 ug |
$489.00
$742.00
|
|
| SC128091 | SYN2 (untagged)-Human synapsin II (SYN2), transcript variant IIa | 10 ug |
$543.00
|
|
Citations
IFITMs Inhibit Cell Fusion Mediated by Trophoblast Syncytins
Zani, Zhang, Kenney et al
bioRxiv (2019)
Zani, Zhang, Kenney et al
bioRxiv (2019)
Snippet: and myc-FLAG-tagged Syncytin-1 and 2 expression constructs in the pCMV6 vector were purchased from Origene. pFR-Luc and pBD-NFkB (Agilent) were co-transfected with plasmids as indicated in Fig 3A. Cell-to-cell fusion assays were performed as outlined and View as HTML
Showing 1-1 of 1 papers.
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