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ADSS1 (NM_152328) Human Tagged ORF Clone

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SKU
RC208536
ADSSL1 (Myc-DDK-tagged)-Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "ADSS1 or NM_152328"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ADSS1
Synonyms ADSSL1; MPD5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208536 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGGACCCGAGCCTCCAACGACCGGCCCCCCGGCGCAGGCGGCGTCAAGCGGGGGCGGCTGCAGC
AGGAGGCGGCGGCGACCGGCTCCCGCGTGACGGTGGTGCTGGGCGCGCAGTGGGGGGACGAGGGCAAAGG
CAAGGTGGTGGACCTGCTGGCCACGGACGCCGACATCATCAGCCGCTGCCAGGGGGGCAACAACGCCGGC
CACACGGTGGTGGTGGATGGGAAAGAGTACGACTTCCACCTGCTGCCCAGCGGCATCATCAACACCAAGG
CCGTGTCCTTCATTGGCAACGGGGTGGTCATCCACTTGCCAGGCTTGTTTGAGGAAGCAGAGAAGAATGA
AAAGAAAGGCCTGAAGGACTGGGAGAAGAGGCTCATCATCTCTGACAGAGCCCACCTTGTGTTTGATTTT
CACCAGGCTGTCGACGGACTTCAGGAAGTGCAGCGCCAGGCACAAGAGGGGAAGAATATAGGCACCACCA
AGAAGGGAATCGGACCAACCTACTCTTCCAAAGCTGCCCGGACAGGCCTCCGCATCTGCGACCTCCTGTC
AGATTTTGATGAGTTTTCCTCCAGATTCAAGAACCTGGCCCACCAGCACCAGTCGATGTTCCCCACCCTG
GAAATAGACATTGAAGGCCAACTCAAAAGGCTCAAGGGCTTTGCTGAGCGGATCAGACCCATGGTCCGAG
ATGGTGTTTACTTTATGTATGAGGCACTCCACGGCCCCCCCAAGAAGATCCTGGTGGAGGGTGCCAACGC
CGCCCTCCTCGACATTGACTTCGGGACCTACCCCTTTGTGACTTCATCCAACTGCACCGTGGGCGGTGTG
TGCACGGGCCTGGGCATCCCCCCGCAGAACATAGGTGACGTGTATGGCGTGGTGAAAGCCTATACCACAC
GTGTGGGCATCGGGGCCTTCCCCACCGAGCAGATCAACGAGATTGGAGGCCTGCTGCAGACCCGCGGCCA
CGAGTGGGGAGTGACCACAGGCAGGAAGAGGCGCTGCGGCTGGCTCGACCTGATGATTCTAAGATATGCT
CACATGGTCAACGGATTCACTGCGCTGGCCCTGACGAAGCTGGACATCCTGGACGTACTGGGTGAGGTTA
AAGTCGGTGTCTCATACAAGCTGAACGGGAAAAGGATTCCCTATTTCCCAGCTAACCAGGAGATGCTTCA
GAAGGTCGAAGTTGAGTATGAAACGCTGCCTGGGTGGAAAGCAGACACCACAGGCGCCAGGAGGTGGGAG
GACCTGCCCCCACAGGCCCAGAACTACATCCGCTTTGTGGAGAATCACGTGGGAGTCGCAGTCAAATGGG
TTGGTGTTGGCAAGTCAAGAGAGTCGATGATCCAGCTGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208536 protein sequence
Red=Cloning site Green=Tags(s)

MSGTRASNDRPPGAGGVKRGRLQQEAAATGSRVTVVLGAQWGDEGKGKVVDLLATDADIISRCQGGNNAG
HTVVVDGKEYDFHLLPSGIINTKAVSFIGNGVVIHLPGLFEEAEKNEKKGLKDWEKRLIISDRAHLVFDF
HQAVDGLQEVQRQAQEGKNIGTTKKGIGPTYSSKAARTGLRICDLLSDFDEFSSRFKNLAHQHQSMFPTL
EIDIEGQLKRLKGFAERIRPMVRDGVYFMYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGV
CTGLGIPPQNIGDVYGVVKAYTTRVGIGAFPTEQINEIGGLLQTRGHEWGVTTGRKRRCGWLDLMILRYA
HMVNGFTALALTKLDILDVLGEVKVGVSYKLNGKRIPYFPANQEMLQKVEVEYETLPGWKADTTGARRWE
DLPPQAQNYIRFVENHVGVAVKWVGVGKSRESMIQLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152328
ORF Size 1371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152328.5
RefSeq Size 1769 bp
RefSeq ORF 1374 bp
Locus ID 122622
UniProt ID Q8N142
Cytogenetics 14q32.33
Domains Adenylsucc_synt
Protein Pathways Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
MW 50.2 kDa
Summary This gene encodes a member of the adenylosuccinate synthase family of proteins. The encoded muscle-specific enzyme plays a role in the purine nucleotide cycle by catalyzing the first step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP). Mutations in this gene may cause adolescent onset distal myopathy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2016
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"NM_152328" in other vectors (5)
SKU Description Size Price
RC208536L3 Lenti ORF clone of Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC208536L4 Lenti ORF clone of Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG208536 ADSSL1 (tGFP-tagged) - Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2 10 ug
$489.00 $657.00
SC108771 ADSSL1 (untagged)-Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2 10 ug
$457.00
SC323993 ADSSL1 (untagged)-Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2 10 ug
$457.00

Plasmid Map for RC208536

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208536
Circular map for RC208536

file-alt Citations

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