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MYBBP1A (NM_014520) Human Tagged ORF Clone

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SKU
RC208514
MYBBP1A (Myc-DDK-tagged)-Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,156.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MYBBP1A
Synonyms P160; PAP2; Pol5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208514 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGCCGGGATCCCGCCCAGCCGATGTCGCCTGGAGAAGCGACGCAGAGTGGCGCCCGGCCTGCCG
ACCGCTATGGCCTATTGAAGCACAGTCGCGAGTTCTTGGACTTCTTCTGGGACATTGCGAAGCCTGAGCA
GGAGACGCGACTTGCGGCCACGGAGAAGCTGCTGGAGTATCTGCGTGGCAGGCCGAAGGGGTCCGAGATG
AAATATGCCCTGAAGCGTCTAATCACGGGACTCGGGGTCGGGCGAGAAACAGCCCGGCCCTGCTACAGTT
TGGCCCTGGCACAGCTGTTACAGTCTTTTGAAGACCTCCCCTTGTGCAGCATCCTGCAGCAGATACAAGA
AAAATATGACCTGCATCAGGTGAAGAAGGCAATGCTGAGACCTGCTCTCTTTGCAAACCTGTTTGGAGTG
CTCGCCCTCTTTCAGTCAGGTCGGCTGGTGAAGGACCAGGAGGCACTGATGAAGTCGGTGAAGCTGCTGC
AGGCCCTGGCCCAGTACCAAAACCACTTGCAGGAGCAGCCCCGGAAGGCCCTGGTGGACATCCTCTCCGA
GGTCTCGAAGGCCACATTGCAGGAGATCCTGCCGGAGGTCCTCAAAGCCGACTTGAATATAATACTCAGC
TCCCCTGAACAGCTAGAGCTCTTCCTCCTGGCCCAGCAGAAGGTGCCCTCCAAGCTCAAGAAGCTGGTGG
GATCCGTGAACCTATTCTCAGATGAGAATGTCCCCAGGCTGGTGAATGTGCTGAAGATGGCCGCCTCCTC
TGTGAAGAAGGACCGCAAGCTGCCCGCCATTGCTCTGGACCTGCTCCGCCTGGCGCTCAAGGAAGACAAG
TTCCCACGGTTCTGGAAGGAGGTGGTGGAACAAGGGCTGCTGAAGATGCAGTTCTGGCCAGCCAGCTACC
TGTGTTTCCGCCTGCTGGGCGCGGCCCTGCCCCTGCTGACCAAGGAGCAGCTGCACCTGGTGATGCAGGG
AGACGTGATCCGCCATTACGGGGAGCACGTGTGCACTGCTAAGCTCCCAAAGCAGTTCAAGTTTGCCCCA
GAGATGGACGATTACGTGGGCACCTTCCTAGAGGGGTGCCAGGATGACCCTGAGCGGCAGCTGGCCGTGC
TAGTGGCCTTCTCATCTGTCACCAACCAAGGCCTCCCTGTCACGCCTACTTTCTGGCGGGTCGTGCGGTT
CCTGAGCCCTCCGGCCCTGCAGGGCTATGTGGCCTGGCTGCGGGCCATGTTTCTCCAGCCAGACCTGGAC
TCCTTGGTTGACTTCAGCACCAACAACCAGAAGAAAGCCCAGGATTCATCGCTCCACATGCCTGAGCGAG
CTGTGTTCCGGCTGAGGAAATGGATCATCTTTCGATTGGTGAGCATTGTGGACAGCCTGCACCTGGAGAT
GGAGGAGGCCTTGACTGAGCAGGTGGCCAGGTTTTGTTTGTTCCACTCGTTCTTTGTCACAAAGAAGCCC
ACATCCCAGATCCCTGAGACAAAGCACCCGTTCTCCTTCCCTTTGGAAAACCAGGCCCGAGAGGCTGTCA
GCAGTGCCTTCTTCAGTCTGTTGCAGACCCTCAGCACGCAGTTCAAGCAGGCACCGGGCCAGACCCAGGG
TGGGCAGCCCTGGACCTACCACCTGGTGCAGTTCGCAGACCTCCTGTTGAATCACAGCCACAACGTGACC
ACCGTGACACCCTTCACTGCGCAGCAGCGCCAGGCCTGGGACCGGATGCTGCAGACTCTGAAGGAGCTGG
AGGCCCACTCCGCAGAGGCCAGGGCTGCTGCCTTCCAGCACCTTCTGCTCCTCGTGGGCATCCACCTCCT
CAAGTCCCCTGCAGAGAGCTGTGACCTGCTGGGTGACATCCAGACCTGCATCAGGAAAAGTCTGGGAGAG
AAGCCCCGCCGGAGCCGCACCAAGACCATCGACCCCCAGGAACCCCCGTGGGTAGAGGTGCTGGTGGAGA
TCTTGCTGGCCCTGTTGGCCCAGCCCAGCCACCTCATGCGCCAGGTGGCCCGGAGCGTGTTTGGCCACAT
CTGCTCCCACCTGACCCCGCGTGCCCTGCAGCTAATTCTGGATGTGCTGAACCCCGAGACCAGTGAGGAT
GAGAATGACCGTGTGGTGGTGACGGACGATTCTGATGAGCGGCGGCTGAAGGGTGCAGAGGACAAGAGCG
AGGAAGGTGAGGACAACAGAAGCTCAGAGAGTGAAGAGGAGAGCGAGGGGGAGGAGAGCGAGGAGGAGGA
GCGCGACGGGGACGTGGATCAGGGCTTCCGGGAACAGCTGATGACCGTGCTGCAGGCTGGGAAGGCGCTG
GGTGGAGAGGACAGTGAGAACGAGGAGGAGCTGGGGGATGAGGCCATGATGGCCCTGGACCAGAGCCTCG
CCAGCCTCTTTGCCGAGCAGAAGCTGCGTATCCAGGCCCGGCGAGACGAGAAGAACAAGCTGCAGAAGGA
GAAGGCTCTGCGGCGCGACTTCCAGATCCGGGTGCTGGACCTGGTGGAGGTGCTAGTGACCAAGCAGCCC
GAGAATGCCCTGGTCCTGGAGCTGCTGGAGCCGCTGCTGAGCATCATCCGGCGCAGCCTGCGCAGCAGCA
GCTCCAAACAGGAGCAGGACCTTCTGCACAAGACGGCGCGCATCTTCACGCACCACCTGTGCCGTGCCCG
GCGCTACTGCCACGACTTGGGTGAGCGCGCAGGGGCCCTGCACGCCCAGGTGGAGCGGTTGGTGCAGCAG
GCTGGCCGCCAGCCCGACTCCCCCACCGCCCTCTACCACTTCAACGCCTCTCTCTACCTGCTCCGGGTCT
TGAAGGGCAACACTGCTGAGGGCTGCGTGCATGAGACACAGGAGAAGCAGAAAGCTGGCACTGACCCCAG
CCACATGCCCACGGGCCCGCAGGCTGCCAGCTGCTTGGACTTGAACCTGGTGACCCGGGTGTACTCGACA
GCACTGAGCTCCTTCCTGACCAAGCGCAACAGCCCCCTCACAGTTCCCATGTTCCTCAGCCTCTTCTCCC
GGCACCCGGTGCTCTGTCAGAGCCTGCTCCCCATCCTGGTCCAGCATATCACGGGCCCGGTGCGGCCCCG
TCGTCAGGCCTGCCTGCTGCTCCAGAAGACCCTGTCCATGCGGGAGGTGAGGTCGTGCTTTGAGGACCCC
GAGTGGAAGCAGCTGATGGGCCAGGTCCTAGCAAAGGTCACCGAGAACTTGCGCGTGCTGGGGGAGGCGC
AGACCAAGGCGCAGCATCAGCAGGCACTGTCCTCCCTGGAGCTGCTCAACGTTCTCTTCAGGACCTGCAA
ACATGAGAAGCTGACCTTGGACCTGACGGTGCTCCTGGGTGTGCTGCAGGGGCAACAGCAGAGCCTACAG
CAGGGGGCACACTCCACCGGCTCCAGCCGCCTGCACGACCTCTACTGGCAGGCCATGAAAACCCTGGGAG
TCCAGCGCCCCAAGTTGGAGAAGAAGGATGCCAAGGAGATCCCCAGTGCCACCCAGAGCCCCATCAGTAA
GAAGCGGAAGAAAAAGGGATTCTTGCCAGAGACGAAGAAGCGCAAGAAACGCAAGTCAGAGGATGGCACG
CCAGCGGAGGATGGCACACCTGCAGCCACCGGCGGGAGCCAGCCCCCCAGCATGGGCAGGAAGAAGAGGA
ACAGGACAAAGGCTAAGGTCCCAGCCCAGGCAAACGGGACGCCAACCACCAAGAGTCCAGCCCCTGGCGC
CCCCACCCGGAGCCCCAGCACCCCTGCCAAATCCCCAAAACTGCAGAAGAAAAACCAGAAGCCGTCCCAG
GTGAATGGAGCTCCCGGGTCCCCCACGGAACCTGCAGGCCAAAAGCAGCATCAGAAGGCTCTTCCCAAAA
AGGGGGTCTTGGGCAAATCACCACTGTCCGCGCTGGCACGGAAAAAGGCAAGGCTGTCTTTGGTCATCAG
GAGTCCCAGCCTGCTTCAGAGTGGGGCCAAGAAGAAAGCACAGGTGAGGAAGGCAGGGAAGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208514 protein sequence
Red=Cloning site Green=Tags(s)

MESRDPAQPMSPGEATQSGARPADRYGLLKHSREFLDFFWDIAKPEQETRLAATEKLLEYLRGRPKGSEM
KYALKRLITGLGVGRETARPCYSLALAQLLQSFEDLPLCSILQQIQEKYDLHQVKKAMLRPALFANLFGV
LALFQSGRLVKDQEALMKSVKLLQALAQYQNHLQEQPRKALVDILSEVSKATLQEILPEVLKADLNIILS
SPEQLELFLLAQQKVPSKLKKLVGSVNLFSDENVPRLVNVLKMAASSVKKDRKLPAIALDLLRLALKEDK
FPRFWKEVVEQGLLKMQFWPASYLCFRLLGAALPLLTKEQLHLVMQGDVIRHYGEHVCTAKLPKQFKFAP
EMDDYVGTFLEGCQDDPERQLAVLVAFSSVTNQGLPVTPTFWRVVRFLSPPALQGYVAWLRAMFLQPDLD
SLVDFSTNNQKKAQDSSLHMPERAVFRLRKWIIFRLVSIVDSLHLEMEEALTEQVARFCLFHSFFVTKKP
TSQIPETKHPFSFPLENQAREAVSSAFFSLLQTLSTQFKQAPGQTQGGQPWTYHLVQFADLLLNHSHNVT
TVTPFTAQQRQAWDRMLQTLKELEAHSAEARAAAFQHLLLLVGIHLLKSPAESCDLLGDIQTCIRKSLGE
KPRRSRTKTIDPQEPPWVEVLVEILLALLAQPSHLMRQVARSVFGHICSHLTPRALQLILDVLNPETSED
ENDRVVVTDDSDERRLKGAEDKSEEGEDNRSSESEEESEGEESEEEERDGDVDQGFREQLMTVLQAGKAL
GGEDSENEEELGDEAMMALDQSLASLFAEQKLRIQARRDEKNKLQKEKALRRDFQIRVLDLVEVLVTKQP
ENALVLELLEPLLSIIRRSLRSSSSKQEQDLLHKTARIFTHHLCRARRYCHDLGERAGALHAQVERLVQQ
AGRQPDSPTALYHFNASLYLLRVLKGNTAEGCVHETQEKQKAGTDPSHMPTGPQAASCLDLNLVTRVYST
ALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRRQACLLLQKTLSMREVRSCFEDP
EWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQ
QGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGT
PAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQ
VNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014520
ORF Size 3984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014520.4
RefSeq Size 4585 bp
RefSeq ORF 3987 bp
Locus ID 10514
UniProt ID Q9BQG0
Cytogenetics 17p13.2
Domains DNA_pol_V
Protein Families Stem cell - Pluripotency, Transcription Factors
MW 148.9 kDa
Summary This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. Alternate splicing results in multiple transcript variants. provided by RefSeq, Sep 2013
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Documents
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"NM_014520" in other vectors (5)
SKU Description Size Price
RC208514L3 Lenti ORF clone of Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,456.00
RC208514L4 Lenti ORF clone of Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2, mGFP tagged 10 ug
$1,456.00
RG208514 MYBBP1A (tGFP-tagged) - Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2 10 ug
$789.00 $1,356.00
SC114999 MYBBP1A (untagged)-Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2 10 ug
$1,157.00
SC323990 MYBBP1A (untagged)-Human MYB binding protein (P160) 1a (MYBBP1A), transcript variant 2 10 ug
$1,157.00

Plasmid Map for RC208514

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208514
Circular map for RC208514

file-alt Citations

Lethal Influenza in Two Related Adults with Inherited GATA2 Deficiency
Sologuren, Martínez-Saavedra, Solé-Violán et al
J. Clin. Immunol. (2018) 38 (4), 513-526 DOI: 10.1007/s10875-018-0512-0
AI Summary: OriGene products GATA2-WT isoform 1 (RC208554) and GATA2-WT isoform 2 (RC208514) were used as expression vectors in HEK293T cells to assess the functional impact of GATA2 mutations via Western blot analysis in this study.
Snippet: Western blots of GATA2 were performed on HEK293T cells transfected with vectors containing GATA2-WT isoform 1 (Origene RC208554) and GATA2-WT isoform 2 (Origene RC208514);mutated vectors containing the variants R396L, R396Q, and R398W were generated
Showing 1-1 of 1 papers.
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