BBS5 (NM_152384) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC208464L3
Lenti ORF clone of Human Bardet-Biedl syndrome 5 (BBS5), Myc-DDK-tagged
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BBS5
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC208464).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152384
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152384.2
RefSeq Size 3196 bp
RefSeq ORF 1026 bp
Locus ID 129880
UniProt ID Q8N3I7
Cytogenetics 2q31.1
Domains DM16
MW 38.8 kDa
Summary This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_152384" in other vectors (6)
SKU Description Size Price
RC208464 BBS5 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 5 (BBS5) 10 ug
$457.00
RC208464L1 Lenti ORF clone of Human Bardet-Biedl syndrome 5 (BBS5), Myc-DDK-tagged 10 ug
$757.00
RC208464L2 Lenti ORF clone of Human Bardet-Biedl syndrome 5 (BBS5), mGFP tagged 10 ug
$757.00
RC208464L4 Lenti ORF clone of Human Bardet-Biedl syndrome 5 (BBS5), mGFP tagged 10 ug
$757.00
RG208464 BBS5 (tGFP-tagged) - Human Bardet-Biedl syndrome 5 (BBS5) 10 ug
$489.00 $657.00
SC127954 BBS5 (untagged)-Human Bardet-Biedl syndrome 5 (BBS5) 10 ug
$457.00

Plasmid Map for RC208464L3

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Cloning scheme for RC208464L3

Circular map for RC208464L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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