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HOGA1 (NM_138413) Human Tagged ORF Clone

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SKU
RC208461
HOGA1 (Myc-DDK-tagged)-Human 4-hydroxy-2-oxoglutarate aldolase 1 (HOGA1), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HOGA1
Synonyms C10orf65; DHDPS2; DHDPSL; HP3; NPL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208461 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGGTCCCCAAGTCTGGTCTTCTGTGAGGCAGGGGCTAAGCAGGAGCTTGTCCAGGAATGTGGGGG
TCTGGGCCTCAGGGGAGGGGAAGAAGGTGGACATTGCGGGTATCTACCCCCCTGTGACCACCCCCTTCAC
TGCCACTGCAGAGGTGGACTATGGGAAACTGGAGGAGAATCTGCACAAACTGGGCACCTTCCCCTTCCGA
GGCTTCGTGGTCCAGGGCTCCAATGGCGAGTTTCCTTTCCTGACCAGCAGTGAGCGCCTCGAGGTGGTGA
GCCGTGTGCGCCAGGCCATGCCCAAGAACAGGCTCCTGCTAGCTGGCTCCGGATGCGAGTCCACTCAAGC
CACAGTGGAGATGACCGTCAGCATGGCCCAGGTCGGGGCTGACGCGGCCATGGTGGTGACCCCTTGCTAC
TATCGTGGCCGCATGAGCAGTGCGGCCCTCATTCACCACTACACCAAGGTTGCTGATCTCTCTCCAATCC
CTGTGGTGCTGTACAGTGTCCCAGCCAACACAGGGCTGGACCTGCCTGTGGATGCAGTGGTCACGCTTTC
CCAGCACCCGAATATTGTGGGCATGAAGGACAGCGGTGGTGATGTGACCAGGATTGGGCTGATTGTTCAC
AAGACCAGGAAGCAGGATTTTCAGGTGTTGGCTGGATCGGCTGGCTTTCTGATGGCCAGCTATGCCTTGG
GAGCTGTGGGGGGCGTCTGCGCCCTGGCCAATGTCCTGGGGGCTCAGGTGTGCCAGCTGGAGCGACTGTG
CTGCACGGGGCAATGGGAAGATGCCCAGAAACTGCAGCACCGCCTCATTGAGCCAAACGCTGCGGTGACC
CGGCGCTTTGGGATCCCAGGGCTGAAGAAAATCATGGACTGGTTTGGCTACTATGGAGGCCCCTGCCGCG
CACCCTTGCAGGAGCTGAGCCCCGCTGAGGAGGAGGCACTGCGCATGGATTTCACCAGCAACGGCTGGCT
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208461 protein sequence
Red=Cloning site Green=Tags(s)

MLGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEENLHKLGTFPFR
GFVVQGSNGEFPFLTSSERLEVVSRVRQAMPKNRLLLAGSGCESTQATVEMTVSMAQVGADAAMVVTPCY
YRGRMSSAALIHHYTKVADLSPIPVVLYSVPANTGLDLPVDAVVTLSQHPNIVGMKDSGGDVTRIGLIVH
KTRKQDFQVLAGSAGFLMASYALGAVGGVCALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVT
RRFGIPGLKKIMDWFGYYGGPCRAPLQELSPAEEEALRMDFTSNGWL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138413
ORF Size 981 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138413.2, NP_612422.2
RefSeq Size 2501 bp
RefSeq ORF 984 bp
Locus ID 112817
UniProt ID Q86XE5
Cytogenetics 10q24.2
MW 35.2 kDa
Summary The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. provided by RefSeq, Nov 2010
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"NM_138413" in other vectors (4)
SKU Description Size Price
RC208461L3 Lenti ORF clone of Human 4-hydroxy-2-oxoglutarate aldolase 1 (HOGA1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC208461L4 Lenti ORF clone of Human 4-hydroxy-2-oxoglutarate aldolase 1 (HOGA1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$600.00
RG208461 HOGA1 (tGFP-tagged) - Human 4-hydroxy-2-oxoglutarate aldolase 1 (HOGA1), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $500.00
SC125992 HOGA1 (untagged)-Human 4-hydroxy-2-oxoglutarate aldolase 1 (HOGA1), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$300.00

Plasmid Map for RC208461

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208461
Circular map for RC208461

file-alt Citations

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