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DNAJC25 (NM_001015882) Human Tagged ORF Clone

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SKU
RC208426
DNAJC25 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJC25
Synonyms bA16L21.2.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208426 representing NM_001015882
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGCCGCTGCTCTCTCCCGGCTGGGGAGCCGGGGCTGCCGGCCGGCGCTGGTGGATGCTGCTGG
CGCCCCTGCTGCCGGCGCTGCTGCTGGTGCGGCCCGCGGGGGCCCTGGTGGAGGGGCTCTACTGCGGCAC
GCGGGACTGCTACGAGGTGCTGGGCGTGAGCCGCTCGGCGGGCAAGGCGGAGATCGCGCGGGCCTACCGC
CAGCTGGCCCGGCGCTACCACCCTGACCGCTACCGGCCCCAGCCCGGAGACGAGGGCCCCGGGCGGACGC
CGCAGAGCGCCGAGGAGGCTTTCCTGCTGGTGGCAACCGCCTACGAGACACTCAAGGATGAAGAAACACG
AAAAGATTATGATTACATGCTGGATCATCCAGAAGAGTACTACAGCCATTACTACCACTACTATAGCAGG
CGCTTGGCCCCTAAGGTGGATGTTAGAGTAGTGATTTTGGTCAGCGTGTGTGCTATTTCGGTGTTTCAGT
TTTTCAGCTGGTGGAATAGCTACAATAAGGCAATCAGCTACCTAGCCACAGTGCCCAAGTACCGTATCCA
AGCTACAGAGATTGCCAAGCAGCAGGGACTGCTCAAAAAAGCCAAAGAAAAAGGCAAAAACAAAAAGTCC
AAAGAAGAAATTCGTGACGAGGAGGAGAACATCATAAAGAACATTATAAAAAGTAAAATAGATATAAAGG
GGGGCTATCAGAAACCCCAAATCTGTGATCTTCTCCTGTTTCAAATTATCTTAGCTCCTTTTCACCTATG
CTCATATATAGTTTGGTATTGTCGGTGGATCTATAATTTTAACATCAAAGGCAAAGAATATGGAGAGGAA
GAGAGATTATACATTATACGTAAATCTATGAAGATGTCAAAGTCTCAATTTGATAGTCTAGAAGATCATC
AGAAAGAAACTTTTCTTAAACGAGAGCTCTGGATCAAGGAGAATTATGAGGTCTACAAGCAAGAACAAGA
GGAAGAATTAAAGAAAAAGTTGGCAAATGACCCCAGATGGAAGAGATACAGGAGATGGATGAAGAATGAA
GGGCCTGGGCGGTTAACATTTGTGGATGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208426 representing NM_001015882
Red=Cloning site Green=Tags(s)

MGAPLLSPGWGAGAAGRRWWMLLAPLLPALLLVRPAGALVEGLYCGTRDCYEVLGVSRSAGKAEIARAYR
QLARRYHPDRYRPQPGDEGPGRTPQSAEEAFLLVATAYETLKDEETRKDYDYMLDHPEEYYSHYYHYYSR
RLAPKVDVRVVILVSVCAISVFQFFSWWNSYNKAISYLATVPKYRIQATEIAKQQGLLKKAKEKGKNKKS
KEEIRDEEENIIKNIIKSKIDIKGGYQKPQICDLLLFQIILAPFHLCSYIVWYCRWIYNFNIKGKEYGEE
ERLYIIRKSMKMSKSQFDSLEDHQKETFLKRELWIKENYEVYKQEQEEELKKKLANDPRWKRYRRWMKNE
GPGRLTFVDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001015882
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001015882.3
RefSeq Size 2268 bp
RefSeq ORF 1083 bp
Locus ID 548645
UniProt ID Q9H1X3
Cytogenetics 9q31.3
Protein Families Transmembrane
MW 42.2 kDa
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"NM_001015882" in other vectors (5)
SKU Description Size Price
RC208426L3 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208426L4 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1, mGFP tagged 10 ug
$757.00
RG208426 DNAJC25 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1 10 ug
$657.00
SC302007 DNAJC25 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1 10 ug
$503.00
SC321828 DNAJC25 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 25 (DNAJC25), transcript variant 1 10 ug
$457.00

Plasmid Map for RC208426

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208426
Circular map for RC208426

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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